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* Corresponding Author
Antibacterial Effectiveness Test of Potato Peel Ethanol Extract (Solanum tuberosum L.) against Lactobacillus acidophilus: An In Vitro Study
https://doi.org/10.22146/mot.81476 Aurelia Krisnadita(1*), Endang Sri Lestari(2), Aris Setyawan(3), Arlita Leniseptaria Antari(4)
(1) Bachelor of Dentistry, Faculty of Medicine, Diponegoro University, Semarang, Jawa Tengah
(2) Department of Microbiology, Faculty of Medicine, Diponegoro University, Semarang, Jawa Tengah
(3) Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Diponegoro University, Semarang, Jawa Tengah
(4) Department of Microbiology, Faculty of Medicine, Diponegoro University, Semarang, Jawa Tengah
(*) Corresponding Author
Abstract
Lactobacillus acidophilus is one of the bacteria that play a major role in the caries process. It is recommended to use cavity cleanser to clean and disinfect caries cavities post-preparation. However, the currently used cavity cleansers, 2% chlorhexidine digluconate and sodium hypochlorite, have limitations. Thus, exploring natural alternatives, like potato peel, as a cavity cleanser material is necessary. This study aims to determine the antibacterial effectiveness of the ethanol extract of potato peel (S. tuberosum L.) on the growth of L. acidophilus. The method used was true experimental laboratories with a post-test-only control group design and broth dilution. The samples were divided into 8 groups, including 6 treatment groups with varying concentrations of extract (100%; 50%; 25%; 12.5%; 6.25%; and 3.125%), positive control, and negative control. Each group was repeated 4 times. The Minimum Inhibitory Concentration (MIC) was determined by observing solution turbidity in the tubes which began to appear clear after 24 hours of incubation, while the Minimum Bactericidal Concentration (MBC) was determined by the absence of bacteria that grew on media after being given the test solution and incubated for 24 hours. The MIC of potato peel ethanol extract on the growth of L. acidophilus could not be determined, while the MBC was at a concentration of 50%. The Kruskal-Wallis test showed that concentration levels had a significant difference in the average number of L. acidophilus colonies. The ethanol extract of potato peel (S. tuberosum L.) has antibacterial effectiveness against the growth of L. acidophilus.
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Balouiri, M., Sadiki, M., & Ibnsouda, S. K, 2016, 'Methods for in vitro evaluating antimicrobial activity: A review', Journal of Pharmaceutical Analysis. 6(2), 71–79. https://doi.org/10.1016/j.jpha.2015.11.005
Bin-Shuwaish, M. S., 2016, 'Effects and effectiveness of cavity disinfectants in operative dentistry: A literature review', J Contemp Dent Pract. 17(10), 867–879.
Cirak, C., & Radusiene, J., 2019, 'Factors affecting the variation of bioactive compounds in Hypericum species', Biologia Futura. 70(3), 198–209.
Golus, J., Sawicki, R., Widelski, J., & Ginalska, G., 2016, 'The agar microdilution method – a new method for antimicrobial susceptibility testing for essential oils and plant extracts', Journal of Applied Microbiology. 121(5), 1291–1299. https://doi.org/10.1111/jam.13253
Hidayah, P., Izzati, M., & Parman, S., 2017, 'Pertumbuhan dan Produksi Tanaman Kentang (Solanum tuberosum L. var. Granola) pada Sistem Budidaya yang Berbeda', Buletin Anatomi Dan Fisiologi (Bulletin Anatomy and Physiology). 2(2), 218–225.
Karpiński, T. M., & Szkaradkiewicz, A. K., 2013, 'Microbiology of dental caries', J Biol Earth Sci. 3(1), M21–M24.
Kepmenkes, 2007, Keputusan Menteri Kesehatan Republik Indonesia Nomor 381/MENKES/SK/III/2007 tentang Kebijakan Obat Tradisional Nasional. Kementerian Kesehatan Republik Indonesia. 1–27.
Kimyai, S., Pournaghi-Azar, F., Naser-Alavi, F., & Salari, A., 2017, 'Effect of disinfecting the cavity with chlorhexidine on the marginal gaps of Cl V giomer restorations', Journal of Clinical and Experimental Dentistry. 9(2), e202–e206. https://doi.org/10.4317/jced.53193
Mohammadi, Z., & Abbott, P. V., 2009, 'The properties and applications of chlorhexidine in endodontics', International Endodontic Journal. 42(4), 288–302. https://doi.org/10.1111/j.1365-2591.2008.01540.x
Ningsih, D. R., & Zusfahair, K. D., 2016, 'Identifikasi senyawa metabolit sekunder serta uji aktivitas ekstrak daun sirsak sebagai antibakteri', Jurnal Molekul. 11(1), 101–111.
Ningsih, N. P., Sari, R., & Apridamayanti, P., 2018, 'Optimasi Aktivitas Bakteriosin yang Dihasilkan oleh Lactobacillus brevis dari Es Pisang Ijo', Jurnal Pendidikan Informatika Dan Sains. 7(2), 233–242.
Nomer, N., Duniaji, A. S., & Nocianitri, K. A., 2019, 'Kandungan senyawa flavonoid dan antosianin ekstrak kayu secang (Caesalpinia sappan L.) serta aktivitas antibakteri terhadap Vibrio cholerae', Jurnal Ilmu Dan Teknologi Pangan. 8(2), 216–225.
Noor, A., Munir, A., Um-Ul-Baneen, F. M., Akhtar, B., & Aslam, B., 2017, 'Phytochemical screening and in-vitro pharmacological evaluation of three vegetable peel extracts', J Toxicol Pharmaceut Sci. 1(1), 67–72.
Noushad, M. C., Ashraf, K., & Suneetha, M. P., 2020, 'Antibacterial efficacy of muringa seed extract and potato peel extract Against Enterococcus faecalis', Contemporary Clinical Dentistry. 11(4), 327.
Nurhayati, T., 2017, 'Uji Efektivitas Ekstrak Etanolik Kulit Kentang (Solanum tuberosum L.) terhadap Pertumbuhan Bakteri Staphylococcus aureus ATCC 25923 secara In Vitro', Fakultas Kedokteran UNISSULA.
Padmasari, P. D., Astuti, K. W., & Warditiani, N. K., 2013, 'Skrining fitokimia ekstrak etanol 70% rimpang bangle (Zingiber purpureum Roxb.)', Jurnal Farmasi Udayana. 2(4), 279764.
Pendit, P. A. C. D., Zubaidah, E., & Sriherfyna, F. H., 2016, 'Karakteristik Fisik-Kimia dan Aktivitas Antibakteri Ekstrak Daun Belimbing Wuluh (Averrhoa bilimbi L.)', Jurnal Pangan Dan Agroindustri. 4(1), Article 1.
Putri, A. M., 2015, 'Pemanfaatan obat herbal topikal pada recurrent aphthous stomatitis dengan pertimbangan manfaat dan keamanannya', Makassar Dental Journal. 4(5).
Rachmawati, F., Nuria, M. C., & Sumantri., 2011, 'Uji Aktivitas Antibakteri Fraksi Kloroform Ekstrak Etanol Pegagan (Centella asiatica (L) Urb) Serta Identifikasi Senyawa Aktifnya', Jurnal Ilmu Farmasi Dan Farmasi Klinik. https://doi.org/10.31942/jiffk.v0i0.372
Rahmadhany, D. (2021). Efektivitas Antibakteri Ekstrak Etanol Kulit Kentang (Solanum tuberosum L.) sebagai Alternatif Bahan Irigasi Saluan Akar terhadap Bakteri Staphylococcus aureus (In Vitro). FKG Universitas Sumatera Utara.
Rahman, F. A., Haniastuti, T., & Utami, T. W., 2017, 'Skrining fitokimia dan aktivitas antibakteri ekstrak etanol daun sirsak (Annona muricata L.) pada Streptococcus mutans ATCC 35668', Majalah Kedokteran Gigi Indonesia. 3(1), 1–7.
Ramayanti, S., & Purnakarya, I., 2013, 'Peran Makanan terhadap Kejadian Karies Gigi', Jurnal Kesehatan Masyarakat Andalas. 7(2), Article 2. https://doi.org/10.24893/jkma.v7i2.114
Setianingrum, I. D., Suardita, K., Subiyanto, A., & Wahjuningrum, D. A., 2017, 'Perbedaan daya pembersih kavitas saponin ekstrak kulit manggis (Garcinia mangostana Linn) 0,78% dan asam sitrat 6% (The difference of 0,78% saponin from mangosteen pericarp extract and 6% citric acid for cleanliness of cavity)', Conservative Dentistry Journal. 7(1), Article 1. https://doi.org/10.20473/cdj.v7i1.2017.6-11
Siwinata, M., Zubaidah, N., & Soetojo, A., 2020, 'The effectivity of cavity cleanser chlorhexidinegluconate 2% and saponin 0.78% of mangoosteen peel', Conservative Dentistry Journal. 10(1), 19–22.
Tim Riskesdas, 2013, Laporan Nasional Riskesdas 2013, Lembaga Penerbit Badan Penelitian dan Pengembangan Kesehatan (LPB).
Tim Riskesdas, 2018, Laporan Nasional Riskesdas 2018, Lembaga Penerbit Badan Penelitian dan Pengembangan Kesehatan (LPB).
DOI: https://doi.org/10.22146/mot.81476
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