EFFECT OF WATER SOLUBLE FRACTION OF COTTON BANANA (Musa paradisiaca L.) ETHANOLIC EXTRACT ON THE BLOOD GLUCOSE LEVELS IN VIVO AND ACTIVE COMPOUNDS IDENTIFICATION

https://doi.org/10.22146/tradmedj.8141

Nunung Yuniarti(1*), Rina Nur Maulawati(2), Suwijiyo Pramono(3)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Banana is one of Indonesian food which has many pharmacological activities. Pisang kapas (Musa paradisiaca L.), one of Indonesian banana, is well known empirically as laxative, so it can be used in the treatment of constipation. It has been reported that both of chloroformic and ethanolic extract of pisang kapas have antidiabetic activities. However, fractination of those are not yet performed and report about their antidiabetic activities are not yet available. Here we investigated the effect of water-dissolved fraction of ethanolic extract of cotton banana to reduce the blood glucose level in rat model and identified its active ingredient. The oral glucose tolerance method with 60 min glucose loading time was used to induce hyperglicemic condition in rats, either in control group or treatment group. Glibenclamid (positive control), sodium carboxymethyl cellulose (negative control) and the fraction were orally given to the rats in a single dose. To measure blood glucose level photometrically, enzimatic reaction by using diagnostic reagent Glucose GOD FS is applied. Paper chromatography was utilized to identify the ingredient of the fraction qualitatively.  The fraction in dose of 0.25 g/kg reduced blood glucose level by 22.28 ± 0.76 %. The fraction contained reductants in addition to glucose, amino acids, and the acids in plants.


Keywords


pisang kapas, Musa paradisiaca, L., antidiabetic, ethanolic extract, oral glucose tolerance method



References

Anonim, 1991, Pedoman Pengujian dan Pengembangan Fitofarmaka, Penapisan Farmakologi, Pengujian Fitokimia, dan Pengujian Klinik, hal. 19-22, 233-239, Kelompok Kerja Ilmiah Phyto Medica, Jakarta

Anonim, 2000, Glucose GOD FS, DiaSys Diagnostic System Gmbh Alte Strasse 9 65558 Holzheim Germany, hal. 1-2

Anonim, 2002, Parameter Standar Umum Ekstrak Tumbuhan Obat, Cetakan Pertama, hal. 5-8, Depkes RI, Direktorat Jenderal Pengawasan Obat dan Makanan, Direktorat Pengawasan Obat Tradisional, Jakarta

Chaplin, M., 1996 Enzym, South Bank University, London. http://www.sbu.ac.uk/biology/enzymes/index.html

Harborne, J.B., 1996, Metode Fitokimia Penuntun Cara Modern Menganalisis Tumbuhan, hal. 4-12, 19-21, 270-292, penerjemah Padmawinata, K., Terbitan Kedua, Penerbit ITB Bandung

Maria, C.L., 1992, Biokimia Nutrisi dan Metabolisme dengan Pemakaian secara Klinis, hal. 40 dan 43, penerjemah Parakkasi, A., UI Press, Jakarta

Nugroho, A.E., 2001, Pengaruh Praperlakuan Brokoli (Brassica oleracea L. var botrytis) terhadap Efek Hipoglikemik Tolbutamid pada Tikus Diabetes Mellitus Tipe II, hal. 45, Thesis, Program Pasca Sarjana UGM, Yogyakarta

Pari L. dan Umamaheswari J., 2000, Antihyperglicemia activity of Musa sapientum, Phytother. Res. J., 14(2): hal. 136-8

Robinson, T., 1995, Kandungan Organik Tumbuhan Tinggi, hal. 14-35, penerjemah Padmawinata, K., Penerbit ITB Bandung

Ros, I.A., 2000, Chemical Constituents, Traditional and Modern Medical Use, Med. Plants of the World J., 2: hal. 319-325

Suharmiati, 2003, Pengujian Bioaktifitas Antidiabetes Mellitus Tumbuhan Obat, Cermin Dunia Kedokteran, 140, hal. 8-10

Thomas, A.N.S., 1992, Tanaman Obat Tradisional, Jilid 2, hal. 93-95, Penerbit Kanisius, Yogyakarta

Wagner, H., Bladt, S., and Zgainski, E.M., 1984, Plant Drug Anal., hal. 299-304, Springer-Verlag Berlin Heidelberg



DOI: https://doi.org/10.22146/tradmedj.8141

Article Metrics

Abstract views : 1463 | views : 29358

Refbacks

  • There are currently no refbacks.




Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada