EFFECT GARGLING WITH CENTELLA’S EXTRACT CONCENTRATION 40% AND 50% TO INHIBIT THE SPECIFIC ACTIVITY GLUTATHIONE S-TRANSFERASE IN SALIVA GINGIVITIS PATIENS

https://doi.org/10.22146/tradmedj.8137

Dhika Paramita Prameswari(1), Juni Handajani(2*)

(1) Faculty of Dentistry, Universitas Gadjah Mada
(2) Faculty of Dentistry, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Glutathione S-transferase (GST) is enzyme in saliva that catalyzes the nucleophilic conjugation reaction of glutathione (GSH) with many diverse electrophilic substrates and has major mechanism in detoxification process of xenobiotics. Pegagan (Centella asiatica (L.) Urban) is contain flavonoid as a xenobiotic component that have an ability to inhibit the specific activity of GST. This study was aiming to understand the effect of gargling with centella’s extract concentration 40% and 50% to inhibit the specific activity of GST in saliva of gingivitis patient. Under ethical clearance from local Ethics Committee from Medical Faculty, Gadjah Mada University, 30 patients of moderate ingivitis divided into two groups: 20 person as treated group were instructed to gargle centella’s extracts concentration 50% and 60%, and 10 person as positive control group was given Hexetidine 0.1%. Every subject was gargling as much as 5 ml every morning (after woke up) and every night (before sleeping) for 5 days constitutively. Saliva was collected before and after treatment. GST’s specific activity was measured using spectrophotometer at 340 nm wavelength and gingivitis status was using Gingival Index (GI). This study showed  that  using centella’s  extract concentration 50% could down regulate the GST’s specific activity saliva and GI score. In conclusion, centella’s extract concentration 50% was indicating better compare with concentration 40% to accelerate of gingivitis healing.


Keywords


gingivitis, saliva, glutathione S-transferase, flavonoid, Centella asiatica (L.) Urban



References

Ambrosone CB, Coles BF, Freudenheim JL, Shields PG. 1999; Glutathione-S-transferase (GSTM1) Genetic Polymorphisms do not Affect Human Breast Cancer Risk, Regardless of Dietary Antioxidants. J Nutr. 129: 565-8

Amerongen AVN. . 1991. Ludah dan Kelenjar Ludah-Arti Bagi Kesehatan Gigi, alih bahasa Prof. drg. Rafiah Abyono, ed.1. Yogyakarta. Gadjah Mada University Pressh.6-8, 38-9

Arini S, Nurmawan D, Alfani F, Hertiani T. 2003;Daya Antioksidan dan Kadar Flavonoid Hasil Ekstraksi Etanol-Air Daging Buah Mahkota Dewa (Phaleria macrocarpa (Scheff.) Boerl.). Buletin Penalaran Mahasiswa UGM. 10(1): 2-6

Burg D, Riepsaame J, Pont C, Mulder G, van de Water B. 2006; Peptide-bond Modified Glutathione Conjugate Analogs Modulate GST-π Function in GSH-conjugation, Drug Sensitivity and JNK Signaling. Biochem Pharmacol. 71: 268-77

Campbell JA, Corrigall AV, Guy A, Kirsch RE. 1991; Immunohistologic Localization of Alpha, Mu, and Pi Class Glutathione S-transferases In Human Tissues. J Cancer. 67(6): 1608-13

De la Torre J, Sholar A. 2006.Wound Healing, ChronicWounds.http://www.emedicine.Com/ plastic /topic477.htm.

Dep Kes RI. Sediaan Galenik. Jakarta. 1986. h.10-2

Devi DG, Lija Y, Cibin TR, Biju PG, Devi VG, Abraham A. 2006; Evaluation of the Protective Effects of Emilia sonchifolia Linn. (DC.) on Perchlorate-Induced Oxidative Damage. J Biol Sci. 6(5): 887-92

Edalat M. 2002; Multiple Functions of Glutathione Transferases: A Study on Enzymatic Function, Regulatory Role and Distribution in Mouse and Man. Acta Universitatis Upsaliensis. Comprehensive Summeries of Uppsala Dissertation from the Faculty of Science and Technology. 729: 36 pp

Fishman, Tamara D. 2007., mhases Of Wound Healing. http://www.medicaledu.com/phases.htm.

Habig WH, Pabst MJ, Jakoby WB. 1974; Glutathione S-transferase, the First Enzymatic Step in Mercaptan Acid Formation. J Biol Chem. 249(22): 7130-9

Herowati R, Adnyana IK. 2001. Pegagan dan PenggunaanTradisional.http://www.pikiran -rakyat.com.

Hollman PCH, Trijp JMP, Buysman MNCP, Gaag MS, Mengelers MJB, Vries JHM, Katan MB. 1997; Relative Bioavailability of the Antioxidant Flavonoid Quercetin from Various Foods in Man. FEBS Letters. 418(1-2): 152-6

Iio M, Kawaguchi H, Sakota Y, Otonari J, Nitahara H. 1993; Effects of Polyphenols, Including Flavonoids on Glutathione S-transferase and Glutathione Reductase. Biosci Biotech Biochem. 10: 1678-80

Kelada SN, Kardia SLR, Walker AH, Wein AJ, Malkowicz SB, Rebbeck TR, 2000; The Glutathione S-Transferase-µ and - Genotypes in the Etiology of Prostate Cancer: Genotype-Environment Interactions with Smoking. CEBP. 9: 1329-34

Lee YL, Hsiue TR, Lee YC, Lin YC, Guo YL. 2005; The Association Between Glutathione S-Transferase P1, M1 Polymorphisms and Asthma in Taiwanese Schoolchildren. Chest. 128: 1156-62

Lim CEL, Matthaei KI, Blackburn AC, Davis RP, Dahlstrom JE, Koina ME, Anders MW, Board PG. 2004., Mice Deficient in Glutathione Transferase Zeta/ Maleylacetoacetate Isomerase Exhibit a Range of Pathological Changes and Elevated Expression of Alpha, Mu, and Pi Class Glutathione Transferases. Am J Pathol. 165( 2): 679-93

Moskaug JS, Carlsen H, Myhrstad MCW, Blomhoff R. 2005; Polyphenols and Glutathione Synthesis Regulation. Am J Clin Nutr. 81(1): 277S-283S

Mueller LA, Goodman CD, Silady RA, Walbot V. 2000; AN9, a Petunia Glutathione S-Transferase Required for Anthocyanin Sequestration, Is a Flavonoid-Binding Protein. Plant Physiol. 123(4): 1561–70

Nijveldt R, van Nood E, van Hoorn DEC, Boelens PG, van Norren K, van Leeuwen PAM, 2001, Flavonoids: a Review of Probable Mechanisms of Action and Potential Application. Am J Clin Nutr. 74: 418-25

Prijantono, 1996., Antiseptik Sebagai Obat Kumur - Peranannya terhadap Pembentukan Plak Gigi dan Radang Gusi. CDK. 113: 28-32

Puy CL. 2006; The Role of Saliva in Maintaining Oral Health and as an Aid to Diagnosis. Med Oral Path Oral Cir Buccal. 11: 449-55

Samuelsson B. 1980; The Leukotrienes: A New Group of Biologically Active Compounds Including SRS-A. TiPS reviews. 227-30

Sastravaha G, Gassman G, Sangtherapitikul P, Grim W. 2006; Herbal Extract as Adjunct in Supportive Periodontal Therapy. Int Poter J Dent Oral Med. 8(10): 1-7

Sata F, Yamada H, Kondo T, Gong Y, Tozaki S, Kobashi G, Kato EH, Fujimoto S, Kishi R. 2003; Glutathione S-transferase M1 and T1 Polymorphisms and the Risk of Recurrent Pregnancy Loss. Mol Hum Reprod. 9(3): 165-9

Snedecor dan Cochran. 1989. Statistical Methods, ed. 8.. IOWA State University. h.289

Snel CAW. 1995.Glutathione Conjugation of Bromo-sulfophtalein in Rat Disposition and Role of Glutathione Availibility. Doctoraalscriptie. Vrije Universiteit, Amsterdam

Somchit MN, Sulaiman MR, Zuraini A, Samsuddin L, Somchit N, Israf DA, Moin S. 2004; Antinociceptive and Antiinflammatory Effects of Centella asiatica. Indian J Pharmacol. 36(6): 377-80

Tanaka T, Kawabata K, Kakumoto M, Makita H, Ushida J, Honjo S, Hara A, Tsuda H, Mori H. 1999; Modifying Effects of a Flavonoid Morin on Azoxymethane-induced Large Bowel Tumorigenesis in Rats. J Carcinogenesis. 20(8): 1477-84

Ujihara M, Tsuchida S, Satoh K, Urade Y. 1988; Biochemical and Immunological Demonstration of Prostaglandandin D2, E2, F2α Formation from Prostaglandin H2 by Various Rat Glutathione S-transferase Isoenzymes. Arch Biochem Biophys. 264: 428-37

Yu QL, Duan HQ, Takaishi Y, Gao WY. A 2006; Novel Triterpene from Centella asiatica. Molecules. 11: 661-5

Zanden JJ, Hamman OB, Iersel MLPS, Boeren S, Cnubben NHP, Bello ML, Vervoort J, Bladeren PJ, Rietjens IMCM. 2003; Inhibition of Human Glutathione S-transferase P1-1 by the Flavonoid Quercetin. Chemico-Biological Interactions. 145(2): 139-48

Zha S, Gage WR, Sauvageot J, Saria EA, Putzi MJ, Ewing CM, Faith DA, Nelson WG, De Marzo AM, Isaacs WB. 2001; Cyclooxygenase-2 Is Up-Regulated in Proliferative Inflammatory Atrophy of the Prostate, but not in Prostate Carcinoma. Cancer Res. 61: 8617-23



DOI: https://doi.org/10.22146/tradmedj.8137

Article Metrics

Abstract views : 342 | views : 7192

Refbacks

  • There are currently no refbacks.




Copyright (c) 2010 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada