ACTIVITY OF AMBON BANANA (Musa paradisiaca var. sapientum) STEM EXTRACT IN OINTMENT FORMULATION ON THE WOUND HEALING PROCESS OF MICE SKIN (Mus musculus albinus)

https://doi.org/10.22146/tradmedj.8136

Bayu Febram(1*), Ietje Wientarsih(2), Bambang Pontjo(3)

(1) Faculty of Veterinary Medicine, Bogor Agricultural University
(2) Faculty of Veterinary Medicine, Bogor Agricultural University
(3) Faculty of Veterinary Medicine, Bogor Agricultural University
(*) Corresponding Author

Abstract


The objective of the present research is to study the activity of banana stem extract in ointment solutions in the acceleration of wound healing process on mice skins. Totally of 45 mice strain DDY 4-6 weeks old were devided in negativecontrol group (placebo ointment), positive control  group (Betadine® ointment) and Ambon banana stem extract ointment.All mice were aseptically wounded 1-1,5 cm in the anterior region of back skin using a sterile scalpel. The wound wassmeared with the ointment. The pathology anatomy observations was done inday 3, 5, 7, 14 and 21 post wounded. Parameters of the gross lesions (pathology anatomy) observations warecolour of the wound, blood coagulations, scab formations and size of the wound. Parameter for (microscopic lesions) histopathology were infiltrations of inflammatory cells (neutrophils, lymphocytes,macrofages), neo-capillarizations, re-epitelization percentage and the  thickness of fibroblast. All quantitative data were measure using ANOVA and continue with Duncan Test, moreover, the qualitative data were presented descriptively. The result shows that gross lesions observations, the extract ointment group was faster in scab formations and covers the wound without trace compared to the negative control group. The statistical test on the infiltrations of inflammatory cells parameter of the extract ointment group significantly different (P<0.05) compared to the negative control group. Histopatologycal observations shows that Ambon banana stem extract in ointment solutions can increase the infiltrations of inflammatory cells, neo-capillary formations, re-epitelizations percentage andacceleration of fibroblast formations. Base on the result the Ambon banana stems extract in ointment solutions can accelerate the wound healing process and it seems that this solution could be developed and uses for the medical purposes.


Keywords


ointment, extract, banana stem, wound healing, mice



References

Ashcroft GS, T Greenwell-Wild, MA Horan, SM Wahl, MWJ Ferguson. 1999. Topical estrogen accelerates cutaneous wound healing in aged humans associated with an altered inflammatory response. Am J Pathol. 155:1137-1146

Ansel HC. 1989. Pengantar Bentuk Sediaan Farmasi. Edisi IV. Alih bahasa : Farida Ibrahim. UI Press : Jakarta : 390-395, 594-600

Best, R., D.A. Lewis and N. Nasser. 1984. The anti-ulcerogenic activity of the unripe plantain banana (Musa species). Br. J. Pharmacol. 82(1):107-116

Block HL. Medicated Applications. Dalam : Gennaro RA, ed. Remington Pharmaceutical Sciences, 18 th ed. Easton, Pennsylvania : Mack Publishing Company, 1990; 1596-608

Djulkarnain HB.1998. Pohon Obat Keluarga. Jakarta: Intisari

Listyanti AR. 2006. Pengaruh Pemberian Getah Batang Pohon Pisang Ambon (Musa parasidiaca var. Sapientum) dalam Proses Persembuhan Luka pada Mencit (Mus musculus albinus). [Skripsi]. Bogor: Fakultas Kedokteran Hewan, Institut Pertanian Bogor

Lee Huang, S., P.L. Huang, P.L. Nara, H. Chen, H. Kung, P. Huang and H.I. Huang. 1996. Plant Protection useful for treating tumors and HIV Infections. US Patent No. 5,484,889

Lewis, G.P. 1986. Mediator of Inflamation. Wright, Bristol

Lewis, D.A., W.N. Fields and G.P. Shaw. 1999. A natural flavonoid present in unripe plantain banana pulp (Musa sapientum L. var. paradisiaca) protects the gastric mucosa from aspirin-induced erosions. J. Ethnopharmacol. 65(3): 283-288

Low, Q.E.H., I.A. Drugea, L.A. Duffner, D.G. Quinn, D.N. Cook, B.J. Rollins, E.J. Kovacs and L.A. DiPietro. 2001. Wound Healing in MIP-alpha-/- and MCP-1-/- Mice. American Journal of Pathology 159:457-463

Maiwahyudi. 1999. Aktivitas getah batang pohon pisang (Musa acuminata) dalam mempercepat proses persembuhan luka pada mencit (Mus musculus). Skripsi S-1. Fakultas Kedokteran Hewan IPB

Pari. 2000. Musa sapientum commonly known as banana is widely used in Indian folk medicine for the treatment of diabetes mellitus. http.www: PubMed/ HerMed/Musa sp. Diakses 12 Maret 2002

Pannangpetch, P., A. Vuttivirojana, C. Kularbkaew, S. Tesana, B. Kongyingoes and V. Kukongviriyapan. 2001. The antiulcerative effect of Thai Musa species in rats. Phytother. Res. 15(5): 407-410

Radini T, 2003. Formulasi Masker Gel dari Lendir lidah buaya (Aloevera Linn).Skripsi S-1 Jurusan Farmasi Universitas Padjadjaran

Sporn. M.B. and A.B Roberts. 1986. Peptide Growth Factors and Inflamation. Tissue Repair and Cancer. Journal of Clinical Investigation. 78: 329

Spector, W.G. and T.D Spector. 1980. An Introduction to General Pathology. 2 nd Ed. Churchill Livingstone. London

Singer AJ, Clark RAF. 1999, Cutaneus wound Healing. N England Journal Medicine. 341 (10):738-154

Versteegh JK. 1988. Petunjuk Lengkap Mengenai Tanam-tanaman di Indonesia dan Khasiatnya sebagai Obat-obatan Tradisionil. Edisi ke-2. Diterjemahkan oleh CD.RS. Bethesda Yogyakarta. Penerbit CD.RS. Bethesda Yogyakarta dan Andi Offset, Yogyakarta

Warzawer-Scwarcz. 1981. Treatment of plantar warts with banana skin. http.www: PubMed/HerMed/Musa sp. Diakses 12 Maret 2002

Wijayakusuma, H.1998 Pisang berkhasiat obat Indonesia, Manfaat dan Penggunannya Rempah,rimpang,dan umbi.Milenia Populer, Jakarta



DOI: https://doi.org/10.22146/tradmedj.8136

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