Effect of Ethanolic Extract of "Jahe Merah" Rhizome in Macrophage Phagocytosis on Mice Infected by Listeria monocytogenes
YS Bintari(1), Sudarsono Sudarsono(2), Agustinus Yuswanto(3*)
(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Ginger is one of some medicinal plants that has been used to make someone still in the comfortable condition e.g. headache, diarrhea, flatulent, cold, cough, and masuk angin. The purpose of this study was to determine effects of pungent principle extract of the “jahe merah” rhizome in the relation of increasing macrophage phagocytic activation. This method was done by using latex beads.( Leijh et al., (1986) The effects of 10 mg/kgBW, 25 mg/kgBW, and 100 mg/kgBW of pungent principle extract of “jahe merah” rhizome ethanolic extract, that extracted by 70%v/v Ethanol was decided according to the number of latex which was phagocyted by macrophages. The conditioning time was done in 20 days. At 15th day, mice were infected by Listeria monocytogenes. The data was analyzed by Kolmogorov-Smirnov, and one way ANOVA with by 95% confidence level. As the specific parameter of the pungent principle extract were: pungent principle spot 1 : 2 = 6.8:1; 3.27%w/w GAE (Gallic Acid Equivalent) as total phenolic concentration, and IC50 14.57 mg/mL. The result was shown that 100 mg/kgBW of 70% ethanolic extract of “jahe merah” rhizome, was capable to increase macrophage activity. The effect of increasing macrophage phagocytosis of 100 mg/kgBW of 70% ethanolic extract of jahe merah was the same with Echinacea extract and Levamisol.
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Anonim, 1999, WHO-Monograph on Selected Medicinal Plant, Vol. I , Geneva, pp.277-297
Anonim,2008, http://goldbamboo.com/topict6386-a1 6Zingiber_officinale .htmL, diakses Agustus 2008
Aeschbach R., Loeliger J., Scott B.C., Murcia A.; Butler J., Halliwell B. ; Aruoma O. I., 1994, Food chem. Toxicol, vol.22 no.1, pp.31-36
Harborne, J.B., 1987, Metode Fitokimia, Cara Modern Menganalisis Tumbuhan, diterjemahkan oleh Kosasih Padmawinata dan Iwang Soedira, Penerbit ITB, Bandung
Hegnauer, R., 1963, Chemotaxonomie der Pflanzen, Band 2, hal. 451-471, Birkhauser Verlag Basel
Jeyakumar SM., Nalini N., Menon VP., 1999, Antioxydant Activity of Ginger (Zingiber officinale Roxb.) in red fed a high fet diet, Med.Sci.Res, 27,5
Leijh, P.J.C., Furtth, R.V. & Zwet, T.L.V., 1986, In Vitro Determination of Phagocytosis and Intracellular Killing by Polymorphonuclear and mononuclear Phagocytes, In: Weir DM, Editor, Cellular Immunology, 2, 74-85 Blackwell Scientific Publication, London
Mella S., 2008 Pengaruh Pemberian Ekstrak Zat Pedas Rimpang Jahe Emprit Yang Disari Dengan Etanol 70% Terhadap Fagositosis Makrofag Pada Mencit Jantan Yang Diinfeksi Dengan Listeria mono-cytogenes, Skripsi, Fakultas Farmasi UGM
Roitt, I.M., 2002, Imunologi (Essential Imunology), diterjemahkan oleh Harahap, A., Edisi VIII, Widya Medika, Jakarta Sudarsono,Agus Pudjoarinto,Imono Argo Donatus, Didik Gunawan,Ngatidjan, Drajat, 1995, Tumbuhan Obat I, Pusat Penelitian Obat Tradisional Universitas Gadjah Mada
Wijayanti, M.A., 1999, Kemampuan fagositosis makrofag peritoneum mencit yang diimunisasi selama infeksi Plasmodium berghei, Berkala Ilmu Kedokteran, 31 (4), 213-218
DOI: https://doi.org/10.22146/tradmedj.8074
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Faculty of Pharmacy
Universitas Gadjah Mada