ASCORBIC ACID CONTENT ON Hibiscus sabdariffa L. CALLUS AT VARIOUS CONSENTRATION OF SUCROSE IN MS MEDIA

https://doi.org/10.22146/tradmedj.8072

Yulita Nurchayati(1*), Fathiyah Afiah R(2)

(1) Facullty of Mathematics and Science, University of Diponegoro
(2) Facullty of Mathematics and Science, University of Diponegoro
(*) Corresponding Author

Abstract


Ascorbic acid is an antioxidant which is found in rosella flower. Its production through conventional method needs long time and wide field, so an alternative method through development of callus invitro is conducted. Sucrose is a main carbon source in MS medium and this sugar plays role as precursor for the formation of ascorbic acid. This study was conducted to determine the effect of sucrose concentration on callus formation and production of ascorbic acid. Sucrose was added to the media at the concentration  of 20 g/L, 30 g/L, 40 g/L and 50 g/L. The culture was incubated for 42 days with twice subculture every 10 days.  Analysis of ascorbic acid was perfomed using iodometry. The results showed that the callus was grow better in sucrose of 20 g/L while the ascorbic acid production was better in sucrose 50 g/L. 


Keywords


rosella callus; ascorbic acid; sucrose



References

Allan, E. 1991. Plant cell and Tissue Culture of Higher Plant. Kluwer Academic Publisher. London.

Amelia, L & Hardiyadi.1999. Upaya Mikropopagasi Tanaman Melon (Cucumis melo L) dengan Penambahan ZPT sitokinin. Biosfera Vol 12: 29-37 Hal:29-36

Carew, D.P. and Krueger, R.J.1977. Catharanthus roseus Tissue Culture: The Effect Medium Modification on Growth and Alkaloid Production. J. Nat. Prod.40:326-336

Damasco, o.P and R. C Barba. 1984. Invitro Culture of Saba Banana (Musa sp.cv. Saba) Phil. Agr, 67,351-358

Gamborg, O. L & G. C Phillips. 1995. Plant Cell, Tissue and Organ Culture Fundamental Methods. Springer Verlag Berlin Heidelberg. German

Giovannoni, J. J. 2007. Completing a Pathway to plant Vitamin Synthesis. The National Academy of Sciences of the USA. PNAS journal 104:9190-9110

Manitto, P. 1981. Biosintesis Produk Alami. Terjemahan : Koensoemardiyah. IKIP Semarang Press. Semarang

Maretzki, A., M. Thom, L.G. Nickell. 1974. Utilization and metabolism of carbohydrates in cell and callus culture. In Street, H.E. (ed). Tissue Culture and Plant Science. London: Academic Press Inc.

Maryani, H dan Kristiana,L. 2005. Khasiat dan Manfaat Rosela. PT Agromedia Pustaka.Jakarta

Merillon, J. M. and K.G. Ramawat, 1999. Mechanism and control. In. Ramawat, K. G and J .M. Merillon (eds). Biotechnology Secondary Metabolites. New Hampchire: Science Publisher, Inc.

Ramawat, K.G. 1999. Production in culture: optimation. In Ramawat,K.G. and J.M. Merillon. (eds). Biotechnology Secondary Metabolites.New Hampchire: Science Publisher, Inc.

Smirnoff, N. 1996. The Function and Metabolism of Ascorbic Acid in Plants. Annals of Botany 78:661-669.



DOI: https://doi.org/10.22146/tradmedj.8072

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