EFFECTS OF FLAVONOIDS ISOLATED FROM ORANGE JASMINE (Murraya paniculata [L.] Jack.) ON HISTAMINE RELEASE FROM MAST CELLS

https://doi.org/10.22146/tradmedj.8066

Agung Endro Nugroho(1*), Sugeng Riyanto(2), Mohamad Aspollah Sukari(3), Kazutaka Maeyama(4)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Science, Universiti Putra Malaysia
(4) Department of Pharmacology, Informational Biomedicine, Ehime University
(*) Corresponding Author

Abstract


Murraya paniculata [L.] Jack. (Kemuning) is a plant that grows widely in some areas of Indonesia. Studies related to this plant have been widely explored especially isolation of its active compounds. The plant contents several active compounds such as flanovoids. In the study, three flavonoid isolated from M. paniculata were evaluated for their effect on histamine release from mast cells (RBL-2H3 cells). These compounds were 3,3’,4’,5,5’,6,7,8-octamethoxyflavone; 3,3’,4’,5,5’,6,7-heptamethoxyflavone and 3, 3’, 4’, 5, 5’, 7–hexamethoxyflavone. The histamine inducers used in the study were DNP24-BSA dan thapsigargin, inducing the histamine release immunologically and non-immunologically, respectively. In the study, heptamethoxyflavone and hexamethoxyflavone did not influence the histamine release from mast cells significantly. However, octamethoxyflavone could increase the histamine release from RBL-2H3 cells in absence and presence the histamine inducers. The flavanoid could increase the release of histamine up to 50 %. Based on the results, polymethoxy moieties at the structure of flavonoid have a significant role to emerge the histamine-release stimulating effect from mast cells. 


Keywords


flavonoid; Murraya paniculata; histamine; mast cells



References

Brayden, D. J., Hanley M. R., Thastrup, O., and Cuthbert, A. W., 1989, Thapsigargin, a new calcium-dependent epithelial anion secretagogue, Br J Pharmacol., 98(3):809-816

Ferracin, R.J., Da Silva, M.F.D.G.F., Fernandes, J.B., Viera, P.C., 1998, Flavonoids from The Fruits of Murraya paniculata, Phytochemistry, 47(3): 393-396

Imai, F., Itoh, Kishibuchi, N., Kinoshita, T. and Sankawa, U., 1989, Constituents of the Root Bark of Murraya paniculata Collected in Indonesia, Chemical Pharmaceutical Bulletin, 37(1):119-123

Kinoshita, T., Tatara, S., Ho, F.C., Sankawa, U. 1989, 3-Prenylindole from Murraya paniculata and Their Biogenetic Significance, Phytochemistry, 28(1):147-151

Kinoshita,T., Wu, J.B., and Ho, F.C. 1996, Prenylcoumarins from Murraya paniculata var.omphalocarpa (Rutaceae) : The Absolute Configuration of Sibirin, Mexoticin and Omphamurin, Chemical Pharmaceutical Bulletin 44 (6):1208-1211

Kong, T.C., Ng, K.H., But, P.P., Li, Q., Yu, S.X., Zang, H.T., Cheng, K.F., Soejarto, D.D., Kan, W.S., and Waterman, P.G., 1986, Source of the Anti-implantation Alkaloid Yeuhchukene in The Genus Murraya, J. of Ethnopharmacology, 15:195-200

Liu, FT., Bohn, JW., Ferry, EL., Yamamoto, H., Molinaro, CA., Sherman, LA., Klinman, NR., Katz, DH., 1980, Monoclonal dinitrophenyl-specific murine IgE antibody: preparation, isolation, and characterization, J Immunol., 124(6), 2728-2737

Metcalfe, DD., Baram, D., Mekori, YA., 1997, Mast Cells, Physiol Rev., 77(4): 1033-1064

Patkar, S. A., Rasmussen, U., and Diamant, B., 1979, On the mechanism of histamine release induced by thapsigargin from Thapsia garganica L. , Agents Actions., 9(1):53-57

Riyanto S., 2003, Phytochemical Studies and Bioactivity Tests of Murraya paniculata Jack, Aegle marmelos Correa, and Zingiber amaricans Blume. Dissertation, Universiti Putra Malaysia

Ryu, S.Y., Kou, N.Y., Choi, H.S., Ryu, H., Kim, T.S. dan Kim, K.M., 2001, Cnidicin, a Coumarin, from the Root of Angelica koreana, Inhibits the Degranulation of Mast Cell and the NO Generation in RAW 264.7 Cells, Planta Med, 67(2) : 172-174

Yamatodani, A., Fukuda, H., Wada, H., Iwaeda, T., Watanabe, T., 1985, High-performance liquid chromatographic determination of plasma and brain histamine without previous purification of biological samples : cation-exchange chromatography coupled with post-column derivatization fluorometry, J Chromatogr., 344 : 115-123

Watanabe, J., Shinmoto, H. dan Tsushida, T., 2005, Coumarin and flavone derivatives from estragon and thyme as inhibitors of chemical mediator release from RBL-2H3 Cells, Biosci. Biotechnol. Biochem, 69(1),1-6



DOI: https://doi.org/10.22146/tradmedj.8066

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