ISOLATION AND IDENTIFICATION OF ANTIOXIDANT COMPOUND OF PERSIMMON LEAVES (Diospyros kaki Thunb.) USING DPPH (2,2 DIPHENYL-1-PIKRILHIDRAZIL) METHOD

https://doi.org/10.22146/tradmedj.8054

Isnindar Isnindar(1), Subagus Wahyuono(2), Erna Prawita Setyowati(3*)

(1) Faculty of Medicine and Nursing, University of Tanjungpura
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Antioxidant is a substance which in small concentrations can significantly inhibit or prevent the oxidation of the substrate. One plant is efficacious as an antioxidant is the persimmon (Diospyros kakiThunb.) where is cultivated widely in East Asia, Spain and Indonesia. In the search for natural antioxidant compounds, have been studied isolation and identification of antioxidant compound of persimmon leaves (DiospyroskakiThunb.) using DPPH (2,2-diphenyl-1-pikrilhidrazil) method. The extraction of persimmon leaves was carried out by maceration method with wasbenzen. Extracts obtained by evaporating the solvent wasbenzenon a rotary evaporator and were then re-macerated with methanol. Antioxidant activity of extracts was evaluated with DPPH 0.2% method. Active extract was partitioned with chloroform, methanol, distilled water and tested for antioxidant activity by DPPH 0.2% method. The active compound was purified byPTLC and the purified active isolates was then confirmed by TLC. The antioxidant activity (IC50) active isolates were analyzed using spectrophotometry. Analysisof active isolates resulted the antioxidant activity (IC50) of 100.00 ug / ml. UV-Vis spectrum of the active isolates showed absorption at λmax 285 and 401 nm. Infrared spectra (KBR) showed absorption at 3446 cm-1 (OH), 2926 cm-1 (CHaliphatic), 1456 cm-1 (CH2), 1384 cm-1 (CH3), 1255 cm-1, and 1115 cm-1 (C-0-C), 1631 cm-1 (C=Caromatic). GC-MS spectra gave two peaks with retention time 11.408 minutes with a similarity index of 79.243% with the molecular ion (M)+ appeared at m/z 178, 163, 147. These spectra are expected to be methyl eugenol. Furthermore, the peak with retention time 12.982 minutes with a similarity index of 20.757%, producing a mass spectrum with molecular ion peaks (M)+appeared at m/z 192, 177, 161. These spectra are expected to be myristicin compound.


Keywords


Leaf persimmon (Diospyros kaki Thunb.), isolation, antioxidant, DPPH (2,2-diphenyl-1-pikrilhidrazil)



References

Cavin A, Hostettmann K, Dyatmyko W and Potterat O (1998). Antioxidant and lipophilic constituents of Tinospora crispa. Planta Med. 64: 393-396

Chefetz B, Salloum AP, Deshmukh and Hatcher PG (2002). Structural components of humic acids as determined by chemical modifications and carbon-13 nmr, pyrolisis and thermochemolysis gas chromatography /mass spectrometry. Soil Science Society of America Journal. 66 : 1159-1171

Chen CY, Wang HM, Chung SH, Lo WL, Yang WL and Yang SC (2010). Chemical constituents from the roots of Cinnamomum subavenium. Chemistry of Natural Compounds. Vol 46. No.3

Chen NX, Fan FJ, Yue X, Wu RX, Li TL (2008). Radical scavenging activity and phenolic compounds in persimmon (Diospyros kaki L. Cv. Mopan). JFS c: food chemistry (73): Nr.1

Creswell CJ, Runquist OA and Campbell MM (1982). Spectrum analysis of organic compound an introductory programmed text. Diterjemahkan ke dalam Bahasa Indonesia oleh Kosasih Padmawinata dan Ny. Iwang Soediro. Penerbit ITB. Bandung

Donald G and Barceloux DG (2008). Nutmeg (Myristicae fragrans Houtt.). Article Medical Toxicology of Natural Substances. Pp 67-70

Demirezer LO, Kruuzum UzA, Bergere I, Schiewe HJ and Zeeck A (2001). The structures of antioxidant and cytotoxic agents from natural source : antraquinones and tannin from roots of Rumex patientia. Phytochemistry, 58: 1213-1217

Ercisli S, Akbulut M, Ozdemir O, Sengul M and Orhan E (2008). Phenolic and antioxidant diversity among persimmon (Diospyros kaki L) genotypes in turkey. I. Journal of Food Sciences and Nutrition. 59 (6): 477-482

Fessenden RJ and Fessenden JS (1999). Kimia Organik. Jilid II. diterjemahkan oleh Pujaatmaka AH, Penerbit erlangga. Jakarta

Gulcin I, Uguz MT, Oktay M, Beydemir S and Kufrevioglu OI (2004)

Evaluation of the antioxidant and antimikrobial activities of clary sage (salvia sclarea L.), Turki. I. Agric. For. 28: 25-33

Halliwel B, and Gutteridge JMS (1999). Free radicals in biology and medicine, 3th ed. Pp. 1-231, 353-425. Oxford University Press. Inc. New York

Harborne JB (1987). Metode fitokimia. Penuntun cara modern menganalisis tumbuhan. Diterjemahkan oleh Kosasih Padmawinata dan Iwang Sudiro. Penerbit ITB. Bandung

Jang CI, Jo KE, Bae SM, Lee JH, Jeon IG, Park E, Yuk GH, Ahn HG, Lee CS (2010). Antioxidant and antigenotoxic activities of different part of persimmon (Diospyros kaki cv fuyu) fruit. J. Medicinal Plants Res. (4) 2. Pp: 155-160

Kim SY, Jeong MS, Kim JS, Jeon IK, Park E, Park RH, Lee CS (2006). Effect of head treatment on the antioxidative and antigenotoxic activity of extracts from persimmon (Diospyros kaki L). Bioschi. Biochem, 70(4): 999-1002

Kwon YS and Kim CM (2003). Antioksidant constituents from the steam of sorghum bicolor. Arch. Pharm. Res. 26 (7): 535-539

Maslarova NVY (2001). Inhibiting oxidation cited in Pokorny J, Yanishlieva N and Gordon M. Antioxidant in food. Practical Applications. 42-48. CRC Press. New York

Moon KJ and Shibamoto T (2009). Antioxidant assay for plant and food components. J. Agric. Food Chem. 57 (5): 1655-1666

Pavia DL, lampman GM and Kriz-jr GS (1997). Introduction to spectroscopy: a guide for student of organic chemistry. 13-223. WB. Saunders Company. Philadelphia

Pokornya J, Yanishlieva N and Gordon M (2001). Antioxidants in food. Practical Applications. 1-123. Wood Publishing Limited. Cambridge. England

Silverstein RM, Bassler GC and Morril TC (1981). Spectrometric Identification of Organic Compound. Fifth Edition. John Wiley and Sons. New York



DOI: https://doi.org/10.22146/tradmedj.8054

Article Metrics

Abstract views : 232 | views : 1018

Refbacks

  • There are currently no refbacks.




Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada