THE ACTIVITY OF RADICAL SCAVENGING OF 2,2-DIPHENYL-1-PYCRILHYDRAZIL BY ETHANOLIC EXTRACTS OF (Tinospora crispa (L.) Miers) STEM AND ITS FRACTIONS

https://doi.org/10.22146/tradmedj.8051

Tatang Irianti(1*), Andayana Puspitasari(2), Ema Suryani(3)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


We have investigated the antiradical activity of ethanolic extracts of Brotowali stem (Tinospora crispa (L.) Miers) using radical scavenging assay or DPPH radical. Thin layer chromatograms were also studied to estimate the group of compounds that have antiradical activity. The ethanolic extract, ethyl acetate and water fractions were showed coumarin and flavonoid. The DPPH antiradical efficiency values of ethyl acetate fraction was the highest antiradical activity namely 200 µg/ mL of this fraction was able to inhibit 53.57% DPPH radicals.

 


Keywords


radical scavenging, Tinospora crispa (L.) Miers, DPPH



References

Cavin, A., Hostettmann, K., Dyatmyko, W., & Potterat, O., 1998, Antioxidant and Lipohilic Constituents of Tinospora crispa, Planta Medica 64 (5), 393-396

Chantong, B., Kampeera, T., & Sirimanapong, W., 2008, Aktivitas Antioksidan Brotowali, http://www.actahort.org/members/showpdf?booknrarnr=786_9, 20 Juni 2009

Cuvelier, M.E., Richard, H., & Besset, C., 1991, comparison of the Antioxidant Activity of Some Acid Phenols: Structure-Activity Relationship, Biosci. Biotech. Biochem., 56 (2), 324-325

Donatus, I.A., 1994, Antaraksi Kurkumin dengan Parasetamol Kajian terhadap Aspek Farmakologi Perubahan Hayati, Disertasi, Fakultas Pasca Sarjana UGM, Yogyakarta

Dweck, A.C. & Cavin, J., 2007, Andawali (Tinospora crispa), Georges Bizet, 20, 32-35

Fagliano, V., 1999, Method for Measuring Antioxidant Activity and Its Application to Monitoring the Antioxidant Capacity of Wine, J.Agric. Food. Chem, 4, 1035-1040

Gitawati, R., 1995, Radikal Bebas : Sifat dan Peran dalam Menimbulkan Kematian Sel, Cermin Dunia Kedokteran, 102, 33-35

Halliwel, B. & Gutteridge, J.M.C., 1992, Free Radical in Biology and Medicine, 3rd edition, 796-798, Oxford University Press, New York.

Harborne, 1987, Metode Fitokimia, Penuntun Cara Modern Menganalisis Tumbuhan, 5-6, Penerbit ITB, Bandung

Jork, H., Funk, W., Fischer, W., & Wimmer, H., 1989, Thin Layer Chromatography Reagents and Detection Methods, 377, Bassel, New York

Kahkonen, M.P., Hopia, A.I., & Fuorella, H.C., 1999, Antioxidant Activity of Extract Containing Phenolic Compound, J. Agric. Food Chem, 47, 3954-3962

Machek, K., 1972, Pharmaceutical Application of Thin Layer and Paper Chromatography, 563-565, Elsevier Publishing Company, London

Majeed, M., Badmaev, V., Shivakumar, U., & Rajendran, R., 1995, Curcuminoid: Antioxidant Phytonutrient, 32-63, Nutri Science Publisher Inc., Piscataway, New Jersey

Molyneux, P., 2004, The Use of Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity, J. Sci. Technicol., 26(2), 211-219

Pokorni, J., Yanishlieva, N., & Gordon, M., 2001, An Antioxidant in Food Practical Application, 2, 10-12, 17, 44-45, 101, 107-108, Woddhead Publishing Ltd., England

Rohman, A., Sugeng, R., & Diah, U., 2006, Aktivitas Antioksidan, kandungan fenolik total, dan kandungan flavonoid total ekstrak etil asetat buah mengkudu serta fraksi-fraksinya, Majalah Farmasi Indonesia, 17(3), 136-142

Stahl, E., 1985, Analisis Obat Secara Kromatografi dan Mikroskopi, diterjemahkan oleh Kosasih Padmawinata, 1-17, Penerbit ITB, Bandung.

Wagner, H., Bladt, S., & Zgainski, E.M., 1996, Plant Drug Analysis, 23-26, Springer-Verlag Berlin Hiedelberg, New York



DOI: https://doi.org/10.22146/tradmedj.8051

Article Metrics

Abstract views : 282 | views : 7216

Refbacks

  • There are currently no refbacks.




Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada