THE ANTIDIABETICS OF COMBINATION METFORMIN AND PURIFIED EXTRACT OF Andrographis paniculata (Burn).F.NESS IN HIGH FRUCTOSE-FAT FED RATS

https://doi.org/10.22146/tradmedj.8049

Eka Siswanto Syamsul(1*), Agung Endro Nugroho(2), Suwijiyo Pramono(3)

(1) Faculty of Pharmacy, Universitas Gadjah Mada Academy of Pharmacy Samarinda
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


A. paniculata is a plant that can be used to overcome the disease diabetes. In DM there is a possibility of the use of therapy with oral hypoglycemic drugs, for example: metformin. This research was conducted using a complete random method indirectional pattern. This study aims to determine the potential of the combination of purified extract of sambiloto herbs and enhancement effects of metformin on blood glucose levels decrease in insulin resistant rats. Test animals used were divided 4 groups, group 1: metformin 45 mg / kg (positive control), group 2: purified extract of sambiloto herbs 434.6 mg/ kg, group 3: a combination of metformin 45 mg / kg BW with the extract of 434.6 mg/ kg (combination 1), and group 4: metformin 22.5 mg/ kg body weight with extract 434.6 mg/ kg (combination 2). Test animals are insulin resistant type 2 DM was made by administering 1.8 g fructose / kg body weight and fat diet for 50 days. Assay of glucose using a reagent kit. Observation of GLUT-4 expression in muscle cells by using immunohistochemical techniques. Insulin resistance rats was tested by using three parameters: (1) testing of blood glucose levels preprandial and postprandial (2) the hypoglycaemic activity of glibenclamide, and (3) observations of GLUT-4 protein expression in muscle tissue. Test results show that the animals had insulin resistant. Results, in this research antidiabetic activity assay indicate that the hypoglycaemic for combination 1 and combination 2 lower compared to a single administration of metformin or purified extract (p<0,05). From the above results concluded that the combination of sambiloto extract purified with metformin does not increase the potency of antidiabetic in single use.


Keywords


A. paniculata, metformin, insulin resistance



References

Agoes, A., Kamaluddin, Chaidir, Y., Munaf., S., Nattadiputra, S., Yodhian, L., Tanzil, S., Azis, S., Theodorus., 2009, Kumpulan Kuliah Farmakologi FK Universitas Sriwijaya. Penerbit EGC, Jakarta. Hal 400-451, 600-617, 710-718

Ameh., Sunday., Obodozie., Obiageri., Inyang., Uford., Abubakar., Mujibata., Garba., Makaji., 2007, A Normative Study of Nigerian Grown “Maha Tita” A. Paniculata, Int. J. Drug. Dev. & Res. 2 (2) : 291-299

.Chao, W.W., and Lin, B.F., 2010, Isolation and Identification of Bioactive compounds in Andrographis paniculata (Chuanxinlian), Chin. Med. J. 5:1-15

Chi, T.C., Liu, I.M., Cheng, J.T., 1999, Less of insulin desensitization in sympathetic nerve terminals from Wistar rats with insulin resistance, J. Auto. Nerv. Sys., 80: 80–84

Chien, C.F., Wu, Y.T., Lee, W.C., 2010, Herb-drug Interaction of A. paniculata Extract and Andrographolide on the Pharmacokinetics of Theophylline in Rats. J. Chem-Biol. Interact., 184 (3): 458-465

Dandu, A.M. dan Inamdar, N.M., 2009, Evaluation of beneficial effects of antioxidant properties of aqueous leaf extract of Andrographis paniculata in STZ-induced diabetes, J. Pharm. Sci., 22(1):49-52

Depkes RI., 2009, Farmakope Herbal Indonesia, Departemen Keseharan RI, Jakarta

Geurin J.C., and Reveillere H.P., 1989, Orthosiphone stamineus as a potent source of methylripario chromene A, J. Nat. Prod., 52(1): 171-173

Harkness, R., 1989, Interaksi Obat (terjemahan), Penerbit ITB, Bandung.

List, P.H., and Schmidt, P.C., 1989, Phytopharmaceutical Technology, Florida, CRC Press. p53-56

Niranjan, A., Tewari, S.K., Lehry, A., 2010, Biological activities Of Kalmegh (A. paniculata Ness) and its active principles, Ind. J. Nat. Prod. Res., 1(2): 125-135

Reyes, B.A., Bautista, N.D., Tanquilut, N.C., Anunciado, R.V., Leung, A.B., Sanchez, G.C., Magtoto, R.L., Castronuevo, P., Tsukamura, H., Maeda, K.I., 2006, Anti-diabetic potentials of Momordica charantia and Andrographis paniculata and their effects on estrous cyclicity of alloxan-induced diabetic rats. J. Ethnopharmacol. 105 (1-2): 196-200

Saltiel, A.R., Khan, C.R., 2001, Insulin signaling and the regulation of glucose and lipid metabolism, Nature, 414: 799-806

Sriningsih, Adji, S.W., Sumaryono, W., Wibowo, A.E., Caidir, Firdayani, Kusumaningrum, S., Kartakusuma, P., 2002, Analisa Senyawa Golongan Flavonoid Herba Tempuyung (Sonchus arvensis L.), JSTF. Hal 21-25

Subramanian, R., Asmawi, M.Z., Sadikun, A., 2008, In vitro alpha-glucosidase and alpha-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide. Acta, J. Biochem. Pol., 55(2):391-398

Sudarsono, Pudjoarinto, A., Gunawan, D., Wahyuono, S., Donatus, I.A., Drajad, M., Wibowo, S., Ngatidjan, 2006, Tumbuhan Obat I. Pusat Penelitian Obat Tradisional, Universitas Gadjah Mada, Yogyakarta. Hal 25-28

Sukandar, E.Y., Andrajati, R., Sigit, J.I., Adnyana, I.K., Setiadi, A.A.P., Kusnandar, 2008, ISO-Farmakoterapi. PT.ISFI Penerbitan, Jakarta

Sule, Q.U., Ahmed, O.A., Samah, Omar, M.N., 2010, Screening for Antibacterial Activity of Andrographis paniculata Used in Malaysian Folkloric Medicine: A Possible Alternative for the Treatment of Skin Infections. Malay. J. Med. Sci., Pubmed

Suryawati, S., 1995, Efek Samping Obat Edisi kedua. Pusat Studi Farmakologi Klinik dan Kebijakan Obat UGM, PT. Karipata, Yogyakarta, hal 245-270

Sutanegara, D., 2006, Ilmu Penyakit Dalam Jilid III, edisi FKUI, Jakarta. Hal: 1034-1042

Tatro, D.S, 2001, Drug Interaction Facts, Facts and Comparison A Wolter Kluwer Company, St. Louis Missouri, USA

Thama, S.Y, Sadikuna, I. Zharia, Haughtonb, P.J., Asmawia, M.Z., 2008, Studies on diuretic and hypouricemic effects of Orthosiphon stamineus methanol extracts in rats, J. Med. Food., 11(2), p. 362–368

Tjay, T.H., dan Raharja, K., 2006, Obat - Obat Penting, Dirjen POM, Departemen Kesehatan Republik Indonesia, Jakarta

Yu, E.C., Hung, C.R., Chen, W.C., Cheng, J.T., 2003, Antihyperglycemic effect of andrographolide in streptozotocin-induced diabetic rats, J.Plant. Med. 69 (12): 1075-1079

Yulinah, E., Sukrasno, Fitri, M.A., 2011, Aktivitas Antidiabetika Ekstrak Etanol Herba Sambiloto (Andrographis paniculata Nees (Acanthaceae)), JMS ITB Vol. 6

Zhang, X.F., and Tan, B.K. 2000. Antihyperglycemic and anti-oxidant properties of Andrographis paniculata in normal and diabetic rats. J. Clin. Exp. Pharmacol. J. Physiol. 27 (3): 58-63

Zhang, Z., Jiang, J., Yu, P., Zeng, X., Larrick, J.W., Wan, Y. 2009. Hypoglycemic and beta cell protective effects of andrographolide analogue for diabetes treatment. J. Transl. Med. 7: 62-69



DOI: https://doi.org/10.22146/tradmedj.8049

Article Metrics

Abstract views : 1220 | views : 11998

Refbacks

  • There are currently no refbacks.




Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada