ANALYSIS ANTIOXIDANT ACTIVITY AND TOTAL ANTHOCYANIN CONTENT IN EXTRACT AND LIPOSOME OF ROSELLE (Hibiscus Sabdariffa L.) CALYX

https://doi.org/10.22146/tradmedj.8048

I Gede Agus Juniarka(1*), Endang Lukitaningsih(2), Sri Noegrohati(3)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The form of liposome formulations is one of strategies to overcome the limitations of anthocyanin extract stability of roselle calyx (Hibiscus sabdariffa L.). In this study, antioxidant comparative analysis of antioxidant activity and total anthocyanin content from rosella extract and its liposomes form was conducted. Preparation of extract made by maceration using the best solvent consisted of methanol : formic acid (97:3) (v/v). From this procedure, the obtained extract was about 26.40% and antioxidant activity was 19.99%. Liposome formulations of methanol extract from roselle calyx were prepared by reverse phase method using rosella extract as aqueous phase and egg lecithin as the lipid phase. The stability of exctract and liposome form of rosella calyx was compared. The stability study showed that there was a reduction of antioxidant activity (DPPH test) 11.25 % for the roselle extract, while the liposome form was 0.93% after 30 days storage in low temperature (0-50C) and saturated nitrogen. In addition, total anthocyanin content (pH differential method test) decreased approximately 39.12 % for  the roselle extract and 0.72% for the liposome form. More over, the liposome had lower level of irritation than the form of extract. Therefore, it can be concluded that anthocyanin encapsulated by liposome was more stable, safe, and effective than the anthocyanin of the rosella calyx extract.


Keywords


rosella, liposome, stability, antioxidan anthocyanin



References

Anonim, 2001, Guidelines for testing of chemicals, No. 404: Skin allergy and irritation test guideline, OECD, Paris

Cerezo, Ana B., Elyana Cuevas, P. Winterhalter, M.C. Garcia-Parrilla, A.M. Troncoso, 2010, Isolation, identification, and antioxidant activity of anthocyanin compounds in Camarosa strawberry, Food & Chemistry 123, 574–582

Giusti, M.Monica and Ronald E. Wrolstad, 2001, Characteristic and Measurement of Anthocyanins by UV-Visible Spectroscopy, Current Protocols in Food Analytical Chemistry, John Wiley & Sons, Inc., F1.2.1-F1.2.13

Harbone, J.B., 1957, Spectral Method of Characterization Anthocyanins, Spectra of Anthocyanins, Vol. 70, 22 – 28

Hirunpanich, V., Utaipat A, Noppawan, P. M., Nuntavan, B., Hitoshi, S., Angkana, H., Chuthamanee, S. 2005. Antioxidant effect of aqueous extracts from dried calyx of Hibiscus sabdariffa linn (roselle) in vitro using rat low-density lipoprotein (LDL). Bio. Pharm. Bull., 28(3): 481- 484

Mursyidi, Achmad, 1984, Statistika Farmasi dan Biologi, Ghalia Indonesia, Jakarta, 61-63, 155

Pinsuwan, Sirirat, Thanaporn Amnuaikit, Suwipa Ungphaiboon, Arunporn Itharat, 2010, Liposome-Containing Hibiscus sabdariffa Calyx Extract Formulations with Increased Antioxidant Activity, Improved Dermal Penetration and Reduced Dermal Toxicity, J Med Assoc Thai 2010; 93 (Suppl. 7), S216-S226

Szoka, Francis Jr., And Demetrios Papahadjopoulos, Procedure For Preparation Of Liposomes With Large Internal Aqueous Space And High Capture By Reverse-Phase Evaporation, Proc. Natl. Acad. Sci. USA, Vol. 75, No. 9, 4194-4198

Zakaria, Z., Aziz, R., Lachimanan, Y. L., Sreenivasan, S., and Rathinam, X., 2008, Antioxidant activity of Coleus blumei, Orthosiphon stamnieus, Ocimum basilicum and Mentha arvensis from Lamiaceae family, J.Nat. Eng. Sci., 2, 93-95



DOI: https://doi.org/10.22146/tradmedj.8048

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