The Effectiveness of Sungkai Leaf (Peronema canescens Jack.) Extract Gel on the Collagen Density of Incision Wounds in Vivo

Clarissa Ester Diana Putri(1), Herlina Eka Shinta(2), Fatmaria Fatmaria(3), Elsa Trinovita(4*)

(1) Medical Education Study Program, Faculty of Medicine, University of Palangka Raya, Palangka Raya, Central Kalimantan
(2) Department of Anatomical Pathology, Faculty of Medicine, University of Palangka Raya, Palangka Raya, Central Kalimantan
(3) Department of Pharmacotherapy, Faculty of Medicine, University of Palangka Raya, Palangka Raya, Central Kalimantan
(4) Department of Pharmacotherapy, Faculty of Medicine, University of Palangka Raya, Palangka Raya, Central Kalimantan
(*) Corresponding Author


An incision is an open wound resulting in skin injury and underlying tissues. The wound-healing process involves a proliferative phase that produces collagen. Sungkai leaves (Peronema canescens Jack.) can be used as an alternative therapy for wound healing because it contains flavonoids, tannins, and saponins that stimulate fibroblasts to synthesize collagen. This study aimed to determine the effectiveness of sungkai leaf extract gel in healing incision wounds in vivo based on collagen density scoring. Twenty-five Wistar rats (Rattus norvegicus) were randomly divided into five groups, the negative control group (K1) was given a gelling agent CMC 1%, the positive control group (K2) was given bioplacenton gel 1%, the treatment group (K3, K4, K5) was given a gel sungkai leaf extract concentration of 5, 10, and 15. The treatment is given topically at the incision site of the incision site of 0.1 g once a day for 14 days. The rats were euthanized on the fifteenth day, and the incision area was excised for histopathological preparation. The data obtained were analyzed using Kruskal-Wallis with SPSS 26. The test results showed p=0.010, meaning there is a significant difference (p<0.05) between the entire treatment. Based on these results, the K4 group (Sungkai leaf extract gel concentration of 10%) showed the highest collagen density scoring results compared to other treatments. This study concludes that sungkai leaves effectively heal incision wounds in Wistar rats based on collagen density scoring parameter.


Peronema canescens Jack.; incision wound; wound healing; collagen density; gel

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Andriani, M., 2019, ' Effect of temperature and extraction time of starfruit leaves (Averrhoa Bilimbi L.) against antioxidant activity by UAE ' method, J Food Science and Technology. 8(3):330-340.

Bajo, Y., 2020, ‘For the healing of incision wounds in white rats the effectiveness of karinyu leaf extract concentration’, Dr Diss Wijaya Kusuma Surabaya Univ.

Citra, AE., 2018, ‘Optimization of water fraction gel formula of binahong leaf with gelling agent of sodium alginate and carboxymethyl chitosan combination’, Traditional Medice Journal. 23 (3).

Dasrinal, E., 2022,' Isolation of secondary metabolite compounds from hexane extract of sungkai plant leaves (Peronema canescens Jack) and toxicity test by BSLT method’, Fak Sains dan Teknol Univ Jambi.

Dewi, IP., 2020, 'Effectiveness of garlic bulb water extract gel against healing of burns and cuts’, J Ilm Manuntung. 6(2):215-222.

Dwi, H., 2018, 'Optimization of honey and aloe vera (Aloe vera) gel combination formulation in diabetic gangrenous wound healing', J Farm. 1(1):60-67.

Fadilah, H., 'Literature study of plant effectiveness against wound healing. S’, Edu Masda J. 2022;6(1):1-9.

Farid, N., 2020, 'Formulation of HPMC base gel preparation ethanol extract of Chinese Jatropha leaves (Jatropha multifida) as wound healing in white rat (Rattus norvegicus)’, Progr Stud S1 Farm Fak Farm Univ Megarezky, Makassar, South Selawesi.

Febriana, NA., 2016, ’Secondary metabolites and wound healing effects of pandanus duri fruit ethanol extract in wistar strain white rats’.

Fiska, F., 2022, 'Antibacterial activity test of sungkai leaf extract gel preparation (Peronema canescens Jack.) against Streptoccocus Pyogenes in vitro’.

Fitria, A., 2021, 'Characterization and test of antioxidant activity against nonpolar, semi-polar, and polar extracts from sungkai leaves’, Fak Farm Univ Perintis Indonesia Padang.

Franz, MG., 2008, 'Guidelines to aid healing of acute wounds by decreasing impediments of healing. Wound Repair Regen'. 16(6):723-748.

Gabbiani, 1971, 'Presence of modified fibroblasts in granulation tissue and their possible role in wound contraction’. 549-550.

Steward, NC., 2021, ‘Anti-inflammatory activity test of Dayak onion ethanol extract ointment preparation (Eleutherine palmifolia (L.) Merr) in male white rats',

Jamuna, S., 2012, 'Screening of in vitro antioxidant activity of methanolic leaf and root extracts of hypochaeris radicata L. Asteraceae’', J Appl Pharm Sci. 2(7):149-154.

Latief, M., 2021, ‘Antiinflammatory activity of ethanol extract of sungkai leaf (Peronema canescens Jack) in carrageenan-induced rats’, J Farm Science and Prakt. 7(2):144-153.

Rety, S., 2021, ‘Gel Formulation from Ethanol Extract of the Leaf of White Guava (Psidium guajava L.)’, Traditional Medicine Journal. 26 (3)

Sea, M., 2019, 'Effectiveness of earring ethanol extract ointment administration (Acalypha indica Linn.) on the healing of incision wounds in rats (Mus musculus)’, J Kaji Vet.

Mappa, T., 2013, 'Gel formulation of sasaladahan leaf extract (Peperomia pellucida (L.) H.B.K) and test of its effectiveness against Burns in rabbits (Oryctolagus cuniculus)’, Pharmacon. 2(2):49-56.

Murti, DA., 2017, 'Effectiveness of Jatropha curcas L gum ointment in the epithelialization phase of wound healing of mouse skin incisions (mus musculus) with Masson trichrome staining’, Jimvet. 01(3):465-472.

Noorhamdani, 2015, 'Methanol extract of young Ambonese banana peel (Musa paradisiaca L.) as an antimicrobial against Escherichia coli bacteria in vitro’.

Novitasari, K., 2019, 'Effectiveness of garlic bulb ethanol extract cream (Allium sativum L.) on the number of fibroblast cells in the healing process of male white rat incision wound (Rattus norvegicus L.)’. Univ Palangka Raya, Medical Faculty.

Nugraha, GAF., 2016, 'The effect of 70% ethanol extract of karamunting leaves (Rhodomyrtus tomentosa (Aiton) Hassk) topical on histopathology picture of collagen fiber thickness of Wistar strain white mouse skin incision wound healing', Fak Dokt Univ Tanjungpura.

Paramita, A., 2013, ' The effect of binahong leaf extract ointment (Anredera cordifolia (Ten) Steenis) on the collagen density of white rats (Rattus norvegicus) who suffered burns’, Faculty of Veterinary Medicine Univ Airlangga. 2(2):254-272.

Rahma, A., 2017, ‘Difference of total flavonoids maceration method with sochletting on ramania leaf extract (Bouea macrophylla Griff)', Dentino J Dokt gigi. 1(1):22-27.

Rahmadhani, N., 2020, ‘Effectiveness of red dragon fruit extract cream (Hylocereus polyrhizus) in increasing fibroblast cell count of Grade II burns in white rats (Rattus norvegicus)’, J Med Vet. 3(1):65.

Rahman, U., 'The effect of ethanol extract of sungkai leaves (Peronema canescens Jack) as antiinflammatory on the soles of white rats’, Fak Farm Univ Andalas.

Rohmani, S., 2019, ' Basil leaf extract handsanitizer gel stability and activity test’, JPSCR J Pharm Sci Clin Res. 4(1):16.

Sucita, RE., 2019, 'Ethanol extract of secang wood (Caesalpinia sappan L.) topically effective on the collagen density of white rats’ incision wound healing period, J Med Vet. 2(2):119.

Vifta, RL., 2018, ‘Phytochemical screening, characterization, and determination of total flavonoid content of extracts and fractions of parijoto fruit (Medinilla speciosa B.) ', Pros Semin Nas Unimus.

Widyasanti, A., 2018, ‘'Physicochemical characteristics of sungkai leaf extract using UAE method', J Ilm Agricultural Engineering and Biosist. 6(1):27-38.

Yulianto, R., 2020, 'Effects of methanol extract of ketapang leaves (Terminalia catappa L.) on collagen density in healing of Grade II burns in white rats (Rattus norvegicus)’, J Med Vet. 3(1):82.


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