ANTIBACTERIAL ACTIVITY ESSENTIALS OILS PONTIANAK ORANGE PEELS AGAINST Staphylococcus aureus and Escherichia coli

https://doi.org/10.22146/tradmedj.8045

Rafika Sari(1*), F. Nour Aulia Mustari(2), Sri Wahdaningsih(3)

(1) Department of Pharmacy, Faculty of Medicine, Universitas Tanjungpura, Pontianak
(2) Department of Pharmacy, Faculty of Medicine, Universitas Tanjungpura, Pontianak
(3) Department of Pharmacy, Faculty of Medicine, Universitas Tanjungpura, Pontianak
(*) Corresponding Author

Abstract


The infectious diseases can be caused by the pathogenic bacteria among Staphylococcus aureus and Escherichia coli. One of the plants that can be used as antibacterial is essential oils from Pontianak orange peels (Citrus nobilis Lour. var. microcarpa). This research aims to determine the antibacterial activity essential oils Pontianak orange peels againts the pathogenic bacteria by disc diffusion method. This research carried out by using the experimental Completely Randomized Design (CRD) Factorial and outcome data were analyzed using the program CoStat Two Way ANOVA method and continued by using LSD (Least Significant Different). The results showed that the essential oils from Pontianak orange peels contain flavonoids, saponins, and triterpenoids. The essential oils from Pontianak orange peels have antibacterial activity againts Escherichia coli and Staphylococcus aureus, where the average zone of inhibition obtained from the concentrations from 0,5; 1,5; and 2,5 mg/mL respectively are 15; 16; and 19 mm and 16,33; 18; and 21 mm. The concentration that gives the biggest inhibition zone is 2,5 mg/mL.


Keywords


antibacterial, essential oils, Pontianak oranges peels, S.aureus, E.coli



References

Agusta, A 2000. Cara Sehat dengan Wewangian Alami. Jakarta : Penebar Swadaya.p.31-37

Brock, T.D., Madigan, M.T. 1991. Biology of Microorganisms. Sixth ed. Prentice Hall International, Inc

Chutia, M., Bhuyan, D. P., Pathak, M. G., Sarma, T. C., Boruah P. 2009. Antifungal Activity and Chemical Composition of Citrus reticulate Blanco Essential Oil Againts Phytopathogens from North East India. Food Science and Technology. 42 (2009). p.777-780

Gibson., J.M. 1996. Mikrobiologi dan Patologi Modern untuk Perawat. Jakarta : Penerbit Buku Kedokteran EGC

Gomez.,Kwanchai.A., Gomez., Arturo.A,. 1995. Prosedur Statistik untuk Penelitian Pertanian. Edisi 2. Jakarta : UI Press. p.16-17

Hertiani T., Palupi, I.S., Sanliferianti, Nurwindasari, H.D.2003.Uji Potensi Antimikroba terhadap S. aureus, E. coli, Shigella dysentriae, dan Candida albicans dari Beberapa Tanaman Obat Tradisional untuk Penyakit Infeksi. Pharmacon. 4 (2)

ICMR. 2009. Detection of Antimicrobial Resistance in Common Gram Negative and Gram Positive Bacteria Encountered in Infectious deseases- An Update. ICMR Bulletin ISSN 0377-4910. 39. p.1-3

Mulyani Sri, Susilowati, Dan Maniur Maslan Hutabarat. 2009. Analisis dan Daya Antibakteri Citrus amblycarpa (Hassk) Ochse. Majalah Farmasi Indonesia. 20 (3). p.127-132

Oxoid Microbiology Product (OMP). 2012. Dehydrated Culture Media. Thermo Fisher scientific. Inc.

Pelczar., MJ., S. Chan, E. C. S. 1986. Dasar-Dasar Mikrobiologi. Jilid 1 dan 2. Jakarta: UI Press

Prihatman, Kemal. 2000. Jeruk Siam (Citrus nobilis Lour var microcarpa), Jakarta : Sistem Informasi dan Manajemen Pembangunan di Perdesaan

Radji, Maksum. 2010. Buku Ajar Mikrobiologi : Panduan Mahasiswa Farmasi dan Kedokteran. Jakarta : Penerbit Buku Kedokteran EGC.

Supranto., J. Statistik Teori dan Aplikasi. 2001. Edisi 6. Jilid 2. Jakarta : Erlangga. p.341-342

Wattimena, JR.,dkk.. 1981. Farmakodinamik dan Terapi Antibiotik. Yogyakarta : Universitas Gajah Mada Press



DOI: https://doi.org/10.22146/tradmedj.8045

Article Metrics

Abstract views : 453 | views : 1037

Refbacks

  • There are currently no refbacks.




Copyright (c) 2013 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada