Bioassay Guided Fractionation of Ciplukan (Physalis angulata L.) Monitored by Glucose Consumption Assay and Thin Layer Chromatography on Myoblast Cells

https://doi.org/10.22146/mot.79783

Mae Sri Hartati Wahyuningsih(1), Ketut Shri Satya Wiwekananda(2), Aurelia Priscilla Regita Putri(3), Dwi Aris Agung Nugrahaningsih(4), Mia Munawaroh Yuniyanti(5*)

(1) *) Department of Pharmacology and Therapy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada; *) Herbal Medical Center, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Undergraduate Medicine Program, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Undergraduate Medicine Program, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
(4) *) Department of Pharmacology and Therapy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada; *) Herbal Medical Center, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
(5) Department of Pharmacology and Therapy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Ciplukan (Physalis angulata Linn.) has been used by the community as an anti-diabetic drug. The antidiabetic effect is due to ingredients such as unsaturated fatty acids, alkaloids, flavonoids, saponins, polyphenols, steroids, triterpenoids, monoterpenoids, and sesquiterpenoids. Part of the fruit of P. angulata contains many active substances of flavonoids with the proportion of fruit extract 300 μg/ml is 84%. Therefore the exploration for compounds responsible for antidiabetic activity in P. angulata needs to be done to ensure empirical evidence. The purpose of this study was to find the active fraction of P. angulata L. which has anti-hyperglycemic properties. This study used Myoblast cells as subjects and the Bioassay Guided Fractionation method for separating compound groups through three stages of the extraction, partitioning, and fractionation processes which were monitored using TLC and the Glucose Consumption Assay test. The results showed that the chloroform extract (CHCl3) was more active in lowering glucose levels compared to the methanol extract (MeOH) (4.86% vs -8.74%). MeOH insoluble extract was more active than MeOH soluble extract (5.14% vs -8.52). The fractionation results showed that Fraction I was the most active in lowering glucose levels compared to FII, III, and IV (26.47%; 13.18%; 0.15%; 13.76%). Therefore Fraction 1 which contains a class of flavonoid compounds is a potential candidate to be developed as an antidiabetic agent.


Keywords


Physalis angulata Linn.; antidiabetic; Bioassay guided fractionation; glucose consumption

Full Text:

PDF


References

American Association Diabetes. (2014). Diagnosis and Classification of Diabetes Mellitus. Diabetes Care, 37(Supplement 1, January 2014), 581. https://doi.org/10.2337/dc14-S081

Aoi, W, Naito, Y., and Yoshikawa, T., 2013, Role of Oxidative Stress in Impaired Insulin

Signalling Associated with Exercise- Induced Muscle Damage, Free Radical Biology and Medicine 65: 1265-1272

Baraka-Vidot, J., Navarra, G., Leone, M., Bourdon, E., Militello, V., & Rondeau, P. (2014). Deciphering metal-induced oxidative damages on glycated albumin structure and function. Biochimica et Biophysica Acta - General Subjects, 1840(6), 1712–1724. https://doi.org/10.1016/j.bbagen.2013.12.017

Cutignano, A., Nuzzo, G., Ianora, A., Luongo, E., Romano, G., Gallo, C., … Fontana, A. (2015). Development and application of a novel SPE-method for bioassay-guided fractionation of marine extracts. Marine Drugs, 13(9), 5736–5749. https://doi.org/10.3390/md13095736

Dal, S., & Sigrist, S. (2016). The Protective Effect of Antioxidants Consumption on Diabetes and Vascular Complications. Diseases, 4(3), 24. https://doi.org/10.3390/diseases4030024

Freitas, P. A. C., Ehlert, L. R., & Camargo, J. L. (2017). Glycated albumin: a potential biomarker in diabetes. Archives of Endocrinology and Metabolism, 61(3), 296–304. https://doi.org/10.1590/2359-3997000000272

Hseu YC, Wu CR, Chang HW, Kumar KJ, Lin MK, Chen CS, et al. Inhibitory effects of Physalis angulata on tumor metastasis and angiogenesis. J Ethnopharmacol. 2011 Jun 1;135(3):762-71.

Iwai, K. (2008). Antidiabetic and antioxidant effects of polyphenols in brown alga Ecklonia stolonifera in genetically diabetic KK-Aymice. Plant Foods for Human Nutrition, 63(4), 163–169. https://doi.org/10.1007/s11130-008-0098-4

Kang, C., Jin, Y. B., Lee, H., Cha, M., Sohn, E. tae, Moon, J., … Kim, E. (2010). Brown alga Ecklonia cava attenuates type 1 diabetes by activating AMPK and Akt signaling pathways. Food and Chemical Toxicology, 48(2), 509–516. https://doi.org/10.1016/j.fct.2009.11.004

Maliangkay, H. P., Rumondor, R., & Kantohe, M. (2019). Skrining Fitokimia dan Potensi Antidiabetes Ekstrak Etanol Herba Ciplukan (Physalis Angulata L) pada Tikus Putih (Rattus Novergicus) yang Diinduksi Aloksan. Bio-Edu: Jurnal Pendidikan Biologi, 4(3), 98–107. https://doi.org/10.32938/jbe.v4i3.422

Murali Krishna T. 2013. In Vitro Determination Of Antioxidant Activity Of Physalis angulata Lnn. International Journal Of Pharma And Bio Sciences.No. 3 Vol. 4.Juli 2013. hal. 541 – 549.

Murini, T., Wahyuningsih, M. S. H., Satoto, T. B. T., Fudholi, A., & Hanafi, M. (2018). Isolation and identification of naturally occurring larvicidal compound isolated from zingiber zerumbet (L).J.E. smith. Asian J. Pharm. Clin. Res., 11(2), 189– 193. https://doi.org/10.22159/ajpcr.2018.v11i2.21703

Negre-Salvayre, A., Salvayre, R., Augé, N., Pamplona, R., & Portero-Otín, M. (2009). Hyperglycemia and Glycation in Diabetic Complications. Antioxidants & Redox Signaling, 11(12), 3071–3109. https://doi.org/10.1089/ars.2009.2484

Nowotny, K., Jung, T., Höhn, A., Weber, D., & Grune, T. (2015). Advanced Glycation End Products and Oxidative Stress in Type 2 Diabetes Mellitus, 194–222. https://doi.org/10.3390/biom5010194

Papatheodorou, K., Papanas, N., Banach, M., Papazoglou, D., & Edmonds, M. (2016). Complications of Diabetes 2016. Journal of Diabetes Research, 2016(September). https://doi.org/10.1155/2016/6989453

Popov, A. M., & Krivoshapko, O. N. (2013). Protective effects of polar lipids and redox-active compounds from marine organisms at modeling of hyperlipidemia and diabetes, 2013(May), 543–550. https://doi.org/10.4236/jbise.2013.65069

Quehenberger, P., Bierhaus, A., Fasching, P., Muellner, C., Klevesath, M., Hong, M., … Nawroth, P. P. (2000). Endothelin 1 transciption is controlled by nuclear factor-κB in AGE-stimulated cultured endothelial cells. Diabetes, 49(9), 1561–1570. https://doi.org/10.2337/diabetes.49.9.1561

Rahimi-Madiseh, M., Malekpour-Tehrani, A., Bahmani, M., & Rafieian-Kopaei, M. (2016). The research and development on the antioxidants in prevention of diabetic complications. Asian Pacific Journal of Tropical Medicine, 9(9), 825–831. https://doi.org/10.1016/j.apjtm.2016.07.001

Selvan, K. B., Piriya, S. P., & Vennison, J. S. (2014). Macro Algae (Eucheuma Cottoni and Sargassum Sp.) Are Reservoirs of Biodiesel and Bioactive Compounds. Journal of Chemical and Pharmaceutical Sciences JCHPS Special Issue, 2(2), 974–2115.

Serban, A. I., Stanca, L., Geicu, O. I., & Dinischiotu, A. (2015). Ages-induced IL-6 synthesis precedes RAGE up-regulation in HEK 293 cells: An alternative inflammatory mechanism? International Journal of Molecular Sciences, 16(9), 20100–20117. https://doi.org/10.3390/ijms160920100

Soares MB, Bellintani MC, Ribeiro IM, Tomassini TC, Ribeiro dos Santos R. Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. Eur J Pharmacol. 2003 Jan 10;459(1):107-12

Soenardjo, N. (2011). Aplikasi budidaya rumput laut Eucheuma cottonii (Weber van Bosse) dengan Metode Jaring Lepas Dasar (Net Bag) Model Cidaun. Buletin Oseanografi Marina, 1(1), 36–44. Retrieved from https://ejournal.undip.ac.id/index.php/buloma/article/view/2970

Sulistyowati Y. 2014. The Difference Between Physalin Standardized Extract From Pysalis Angulata. Land Control On Pancreatic Function Of Sprague Dawley Rat Induced By StreptozotocinNicotinamide. Scholars Journal Of Applied Medical Sciences (SJAMS). No. 4B Vol. 2 Hal. 12971301

Sun L, Liu, J., Liu, P., Yu, Y., Ma, L., and Hu, L. Immunosuppression effect of

Withangulatin A from Physalis angulata via heme oxygenase 1-dependent pathways. Process Biochemistry. 2011;46:482–8.

Syarif, R. A., Wahyuningsih, M. S. H., Mustofa, M., & Ngatidjan, N. (2018). Antiplasmodial and onset speed of growth inhibitory activities of Tithonia diversifolia (Hemsley) A gray leaf fractions against Plasmodium falciparum. Trop J Pharm Res, 17(11), 2213–2218. https://doi.org/10.4314/tjpr.v17i11.15

Tripathi, B. K., & Srivastava, A. K. (2006). Diabetes mellitus: Complications and therapeutics. Medical Science Monitor, 12(7), RA130-RA147. https://doi.org/4187 [pii]

Vieira AT, Pinho V, Lepsch LB, Scavone C, Ribeiro IM, Tomassini T, et al. Mechanisms of the anti-inflammatory effects of the natural secosteroids physalins in a model of intestinal ischaemia and reperfusion injury. Br J Pharmacol. 2005 Sep;146(2):244-51.

Wahyuningsih, M. S. H., Mubarika, S., Ganjar, I. G., Wahyuono, S., & Takeya, T. (2017). 5α-Oleandrin reduce Bcl-2 protein and increase Bax protein expression on Hela cervical cancer cell. UnivMed, 36(2), 102. https://doi.org/10.18051/univmed.2017.v36.102-109

Wahyuningsih, M. S. H., Wijayanti, M. A., Budiyanto, A., & Hanafi, M. (2015). Isolation and identification of potential cytotoxic compound from kembang bulan [tithonia diversifolia (Hemsley) a gray] leaves. Int J Pharm Pharm Sci, 7(6), 298– 301

Welsh, K. J., Kirkman, M. S., & Sacks, D. B. (2016). Role of glycated proteins in the diagnosis and management of diabetes: Research gaps and future directions. Diabetes Care, 39(8), 1299–1306. https://doi.org/10.2337/dc15-2727

Winarsi Hery. 2007. Antioksidan alami dan radikal bebas potensi dan aplikasinya dalam kesehatan. Yogyakarta: Kanisius.

Zhang, Q. W., Lin, L. G., & Ye, W. C. (2018). Techniques for extraction and isolation of natural products: A comprehensive review. Chinese Medicine (United Kingdom), 13(1), 1–26. https://doi.org/10.1186/s13020-018-0177-x



DOI: https://doi.org/10.22146/mot.79783

Article Metrics

Abstract views : 948 | views : 1010

Refbacks

  • There are currently no refbacks.




Copyright (c) 2023 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada