INFUSE OF Costus speciosus (KOEN.) JE SMITH LEAF AS AN INHIBITOR OF SPERMATOZOA QUANTITY AND QUALITY OF MALE MICE BALB / C
Ika Puspita Sari(1*), Siti Rahayu(2), Dicky M. Rizal(3)
(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Medicine, Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Costus speciosus (Koen.) J.E. Smith (Pacing) is a plant widely use as traditional contraception. The objective of this study is to evaluate the effect of pacing leaves infusion on Balb/C mice spermatogenesis and its reversibility. This study was conducted on male mice. Mice was divided into 6 groups, 6 mice each group. Pacing leaves infusion was given orally at dose 275, 550, and 1100 mg/kg body weight for 14 days. The control groups received distilled water and another group received corn oil and andriol 5.2 mg/kg body weight. At day 15, 3 mice from all of groups was euthanized and the rest of mice in all of groups was euthanized in day 28 to evaluate the reversibility of spermatogenesis. The observation of spermatogenesis was conducted on quantity and quality of spermatozoa (motility, viability, and morphology). The result showed that pacing leaves infusion at dose 275, 550, and 1100 mg/kg body weight reduced the number of spermatozoa by 16-38 % (p<0.05), but it did not change the viability and morphology of the spermatozoa. At dose 275 and 550 mg/kg body weight, pacing leaves infusion reduced the motility of spermatozoa by 36-39 % (p<0.05). The ability of pacing leaves infusion in reducing the number and motility of spermatozoa is reversible.
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Adnan & Pagarra, B., 2000, Pengaruh Ekstrak Rimpang Tumbuhan Pacing(Costus speciosus, J.E Smith) terhadap Spermatogenesis Mencit (Musmusculus) ICR Jantan, Laporan Penelitian, Jurusan Biologi FMIPA, Universitas Negeri Makasar
Anon, A., 1978, Gossypol-a new contraceptive for men, Chinese Medical Journal, 4:417-428
Anonim, 1994, Penuntun Laboratorium WHO untuk Pemeriksaan SemenManusia dan Interaksi Sperma-Getah Servik, Edisi 3, diterjemahkanoleh Arsyad, K. M. & Hayati, L., Bagian Biologi Medik FakultasKedokteran Universitas Sriwijaya, Palembang
Darszon A, Labarca P, Nishigaki T, &Espinosa F., 1999, Ion Channels in Sperm Physiology. Physiology Review79: 481-510
Devi, V. & Urooj, A., 2010, Nutrient Profile and Antioxidant Components of Costus speciosus Sm. and Costus igneus Nak., Indian Journal of NaturalProducts and Resources 1 (1): 116-118
Furuya, T., Yamagata, S., Shimoyama, Y., Fujihara, M., Morishima, N., & Ohtsuki, K., 1997, Biochemical Characterization of Glyzyrrhizin as an Effective Inhibitor for Hyaluronidases from Bovine Testis, Biol.Pharm.Bull 20(9):973-977
Guyton, A.C. & Hall, J.E, 1997, Textbook of Medicinal Physiology, 9th Edition, W.B. Saunders Company, Philadelphia
Henriksen, K., Kangasniemi, M., Parvinen, M., Kaipia, A. & Hakovirta, H., 1996, In Vitro, Follicle-Stimulating Hormon Prevents Apoptosis and Stimulates Deoxyribonucleic Acid Syntesis in the Rat Seminiferous Epithelium in a Stage-Specific Fashion. Endocrinology Journals, 137 (5): 2141
Ma X.H,&Shi Y.L.1998, Ion Channels of Mammalian and Human Sperm Membrane. Prog Physiol Sci29: 109-14
Muchtaromah, B., 2009, Potensi Ekstrak Buah Pare (Momordica charantia L) Terhadap Spermatogenesis Mencit (Mus musculus), Berkala Penelitian Hayati 3D:57-60
Putranti, O.D., Kastono & Ismaya, 2010, Pengaruh Penambahan Crude Tannin pada Sperma Cair Kambing Peranakan Ettawa yang disimpan selama 14 hari terhadap Viabilitas Spermatozoa, Buletin Peternakan 34(1): 1-7
Rahayu, M., Sunarti, S., Sulistiarini, D., & Prawiroatmodjo, S., 2006, Pemanfaatan Tumbuhan Obat secara Tradisional oleh Masyarakat Lokal di Pulau Wawonii, Sulawesi Tenggara, Biodiversitas 7 (3): 245-250
Satriyasa, B.K., 2009, Fraksi Heksan dan Fraksi Metanol Biji Pepaya Muda Dapat Menghambat Spermatosit Primer Pakhiten Mencit Jantan (Mus musculus), Majalah Obat Tradisional vol. 14 no.47
Shajeela, P.S., Mohan, P.R., Jesudas L.L & Soris, P.T., 2011, Antifertility of Ethanol Extract of Dioscorea seculenta (L.) Schott on Male albino Rats, International Journal of PharmTech Research 3 (2): 946-954
Shi, Y.L., Bai, J.P.& Wang, W.P., 2003, Ion-channels in Human Sperm Membrane and Contraceptive Mechanisms of Male Antifertility Compounds Derived from Chinese Traditional Medicine, Acta Pharmacologica Sinica24(1):22-30
Srivastava, S., Singh, P., Mishra, G., Jha, K.K. & Khosa, R.L., 2011, Costus speciosus (Keukand): A review, Der Pharmacia Sinica 2 (1): 118-128
Tarigan, P., 1980, Beberapa Aspek Kimia Sapogenin Steroid pada Tumbuhan di Indonesia, Alumni, Bandung
Wahyudi, R., Sutarjadi, Wardojo, B.P.E., & Hinting, A., Pengaruh ekstrak diklormetan dan metanol daun Gendarussa vulgaris Ness terhadap motilitas dan viabilitas spermatozoa manusia in vitro. Yogyakarta: Simposium Bahan Obat Alami IX,Yogyakarta;1997.
Winarno, M.W. & Sundari D., 1997, Informasi Tanaman Obat untuk KontrasepsiTradisional, Cermin Dunia Kedokteran No 120: 25-28
DOI: https://doi.org/10.22146/tradmedj.7945
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Faculty of Pharmacy
Universitas Gadjah Mada