Sri Wahdaningsih(1*), Subagus Wahyuono(2), Erna Prawita Setyowati(3)

(1) Department of Pharmacy, Faculty of Medicine and Public Health Sciences, Universitas Tanjungpura Pontianak
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author


Oxidative stress induced by the radicals have been known to affect the occurrence of various degenerative disease. In order to search natural antioxidant compounds, it had been studied the isolation and identification of antioxidant compounds in fern stems (Alsophila glauca J.Sm) using DPPH method (1,1-diphenyl-2-picrylhydrazyl). Fern stem extraction was done by maceration with wasbenzen and metanol. Extracts was obtained by evaporating the wasbenzen solvent with a rotavapor. Material was re-macerated with methanol after wasbenzen evaporation. The same way was done to obtain the metanol extract. These extracts were tested for antioxidant activity using DPPH method with TLC. Active extract was partitioned in 80% methanol. Both 80% methanol soluble and insoluble extract were tested their antioxidant activity using DPPH method (TLC). Active extract was fractionated in vacuum liquid chromatography using a mobile phase with different polarity gradient and different concentrations (wasbenzen:chloroform). Active fraction was isolated by preparative TLC method and purity of obtained compounds was tested by TLC. Antioxidant activity of obtained isolates was tested by DPPH method using spectrophotometry. Compounds known to had antioxidant activity as radical catcher with IC50 178.4µg/mL. 


Stem fern, antioxidant, DPPH (2,2-diphenyl-1-picrylhydrazyl)

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Rohdiana, D. 2001. Radical Scavengers Polyphenol. Majalah Farmasi Indonesia. 12 (1) : 53-58

Amarowich, R., Naczk, M. & Sahidi, F. 2000. Antioxidant Activity of Crude Tannins of Canola and Rapeseed hulls, JAOCS, 77 : 957-961

Rohman, A. & Riyanto, S. Antioxidant Activity of Mengkudu (Morinda citrifolia L.) fruit extrac, Agritech 25 (3) : 131-136

Sofnie, M., Chairul & Sumarny. 2003. Aktivitas Antioksidan Ekstrak Air Daun Tempuyung (Sonchus arvensis L.) Secara Invitro. M.F.I, 14 (4) : 208-215

Molyneux, P. 2004. The Use of Stable Free Radical Diphenylpicrylhidrazil (DPPH) For Estimating Antioxidant Activity. Songklanakrin Journal Science Technologi 26 (2)

Utami, R.F. 2009. Efek Gel Batang Pakis Ikan terhadap Penyembuhan Luka Bakar Buatan pada Kelinci jantan. Karya Tulis Ilmiah. Akademi Farmasi Yarsi Pontianak

Kwon, Y.S., & Kim, C.M. 2003. Antioxidant Constituent from the Stem of Sorghum bicolor. Arch. Pharm. Res. 26 (7) : 535-539

Demirezer, L.O., Kruuzum-Uz, A., Bergere, I., Schiewe, H.J. & Zeeck, A. 2001. The Structures of Antioxidant and Cytotoxic Agents from Natural Source : Antraquinones and Tannin from Roots of Rumex patientia, Phytochemistry. 58: 1213-1217

Blouis, M. S. 1958. Antioxidant Determinations By The Use Of a Stable Free Radical. Nature. 1199-1200

Gandjar. IG & Rohman. A. 2007. Kimia Farmasi Analisis. Cetakan 1. Penerbit Pustaka Pelajar. Jogjakarta


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