THE EFFECT OF MEDIUM ON ENDOPHYTIC FUNGUS IP-2 GROWTH AND PRODUCTION OF ITS ACTIVE INHIBITOR HEME POLYMERIZATION METABOLITE

https://doi.org/10.22146/tradmedj.7753

Indah Purwantini(1*), Wahyono Wahyono(2), Mustofa Mustofa(3), Ratna Asmah Susidarti(4)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Medicine, Universitas Gadjah Mada
(4) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Endophytic fungus IP-2 is the endophytic fungus isolated from the plant Artemisia annua L. This fungus is known to have activity as an inhibitor of heme polymerization. The aim of this study is to examine the suitable medium for the growth and production of the active metabolite and to determine the appropriate time for harvesting the active metabolite. Endophytic fungus was fermented in 4 types of media for 14 days. Sampling was taken every day to obtain the weight of the dry cells which were used for analysis of growth. The media obtained is used to determine their activities as heme polymerization inhibitor. Tests were carried out using the method developed by Bassilico et al (1998). Data of dry cell weight and the percentage inhibition of heme polymerization were analyzed in order to obtain the curve of growth and metabolite production. Both curves will be used to determine the proper time to harvest the active metabolite. The results showed that the PDB was the best medium for endophytic fungus IP-2 growth and was able to produce the active metabolite higher than most other media. In the PDB media, at day-7 showed that active metabolite produced at most so that proper harvest time metabolite is on the 7th day of fermentation.


Keywords


endophytic fungi IP-2, fermentation media, heme polymerization inhibitors



References

Basilico, N., Pagani, E., Monti, D., Olliaro, P., Taramelli, D., 1998, A Microtitrebased Method for Measuring the Haem Polymerization Inhibitory Activity (HPIA) of Antimalarial Drugs. J. Antimicro. Chemother. 42:55-60

Chisti Y, Moo-Young M., 1993, Clean-in-place systems bioreactor :design, validation and operation, ASME Bioeng, Div Publ Bed, 27:5–12

Gunawan, D., dan Mulyani, S., 2004, Ilmu Obat Alam (Farmakognosi) Jilid I.Cetakan Pertama, Penebar Swadaya, Jakarta

Judoamidjojo, M., Darwis, A.A., & Sa’id, E.G., 1992, Teknologi Fermentasi, 118, 79-82, 95-97, Rajawali Pers, Jakarta

Llorens A, Matco R, Hinojo MJ, Logrieco A, Jimenez M., 2004, Influence of the interactions among ecological variables in the characterization of Zearalenone producing isolates of Fusarium spp. Systematic and Applied Microbiology., 27:253–260

Miao Y,Lv., D,Wang, P Wang X-C, Chen J, Miao C, 2006, An Arabidopsis glutathione peroxidase functions as both a redox transducer and a scavenger in abscisic acid and drought stress responses, Plant Cell, 18:2749–66

Munghtin, M., Bray, P.G., Ridley, R.G.,Ward, S.A., 1998, Central Role of Hemoglobin Degradation in Mechanism of Action of 4-aminoquinolines, Quinoline Methanols, and Phenantene Methanols, Antimicrobial Agent Chemotherapy, 42(11):2973-2977

Purwantini, I., Wahyono, Mustofa, dan Asmah R., 2010, Aktivitas Inhibitor Polimerisasi Hem Metabolit Penicillium sp Fungi Endofit yang Diisolasi dari Artemisia annua L, Seminar Nasional Eight StarsPerformance Pharmacyst, Yogyakarta

Ramakrishna A, Ravishankar GA., 2011, Influence of abiotic stress signals on secondary metabolites in plants, Plant Signal Behaviour, 6(11):1720-31

Rosenthal, P.J., 2003, Review Antimalarial Drug Discovery : Old and New Approaches, The Journal of Experimental Biology, 206:3735-3744

Tong, W.Y., Darah, I., Latiffah, Z., 2011, Antimicrobial Activities of Endophytic Fungal Isolates From Medicinal Herb Orthosiphon stamineus Benth, Journal of Medicinal Plants Research Vol. 5(5), pp. 831-836

Waites, M.J., Morgan, N.L., Rockey, J.S., dan Higton, G., 2001, Industrial Microbiology: An Introduction, T.J. International Ltd, Padstow, Cornwall



DOI: https://doi.org/10.22146/tradmedj.7753

Article Metrics

Abstract views : 1470 | views : 4578

Refbacks

  • There are currently no refbacks.




Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada