Combined Spermacoce radiata and Hypselodelphys poggeana Extract (CESH) Protect against Oxidative Stress and Enhances Haematological Parameters in Benign Prostatic Hyperplasia-induced Rats

https://doi.org/10.22146/mot.76400

Robert Ikechukwu Uroko(1*), Favour Matthew Awah(2), Chinedu Aguwamba(3), Mercylyn Ezinne Uche(4), Ikenna Obiwuru(5), Chinomso Friday Aaron(6), Ezichi Favour Ofoezie(7)

(1) Michael Okpara University of Agriculture Umudike, Abia State, Nigeria.
(2) Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria, PMB 7267.
(3) Biochemistry Unit, Department of Chemical Sciences, Faculty of Sciences, Clifford University, Owerrinta, Abia State, Nigeria.
(4) Department of Biochemistry, Faculty of Biological and Physical Sciences, Abia State University, Uturu, Nigeria.
(5) Department of Biochemistry, Faculty of Biological and Physical Sciences, Abia State University, Uturu, Nigeria.
(6) Department of Biochemistry, Faculty of Biological and Physical Sciences, Abia State University, Uturu, Nigeria.
(7) Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria, PMB 7267.
(*) Corresponding Author

Abstract


This study investigated the therapeutic effect of a combined extract of Spermacoce radiata and Hypselodelphys poggeana (CESH) on oxidative markers and haematological parameters in benign prostatic hyperplasia (BPH) induced rats. The study adopted five groups containing equal numbers of rats (n = 6), including normal control, BPH control, Finasteride control, BPH-induced rats treated with 200 mg/kg CESH, and BPH-induced rats treated with 600 mg/kg CESH. The rats were induced BPH by the subcutaneous administration of a 5 mg/kg testosterone propionate injection. At the same time, treatment finasteride and CESH to the respective groups were given orally 60 minutes after the BPH induction for 28 uninterrupted days. The induction of BPH with testosterone propionate injection caused a significant reduction in the serum levels of haematological parameters, including haemoglobin (Hb), packed cell volume (PCV), red blood cells (RBC), and platelet counts of the BPH control compared with normal control. The glutathione (GSH) concentration, glutathione peroxidase (GPx), superoxide dismutase (SOD), glutathione S-transferase, and catalase activities decreased significantly in the BPH control relative to the normal control. The BPH control had elevated white blood cell (WBC), and malondialdehyde (MDA) concentrations contrary to the high WBC and MDA in the normal control and CESH treated BPH induced rats, respectively. Conversely, the Hb, PCV, platelet count, GPx, SOD, catalase, GST, and GSH increased significantly in the finasteride and CESH-treated BPH-induced rats, respectively, compared to the BPH control. These findings show that CESH attenuates adverse effects of BPH on antioxidant parameters and oxidative markers, which may prevent BPH progression.


Keywords


Oxidative stress; Antioxidants parameters; Haematological parameters; Prostate enlargement; Medicinal plants

Full Text:

PDF


References

Aebi, H.E., 1983, ‘Catalase. In: Bergmeyer HU, (ed) Methods of Enzymatic Analysis’ 3rd edn. Deefield Beach, pp. 273-285, Weinheim, Florida.

Agrawal, M., Nahata, A. & Dixit, V.K., 2012, ‘Protective effects of Echinops echi-natus on testosterone-induced prostatic hyperplasia in rats’, Eur. J. Integr. Med. 4, 177-185.

Ajayi, A. & Abrahama, K., 2018, ‘Understanding the role of oestrogen in the development of benign prostatic hyperplasia’, African J. Urol. 24, 93–97.

Bamishaiye, E., Muhammad, N. & Bamishaiye, O., 2009, ‘Haematological parameters of albino rats fed on tiger nuts (Cyperus esculentus) tuber oil meal-based diet. Internet J. Nutr. Wellness. 10, 1-5.

Burkill, H.M., ‘The Useful Plants of West Tropical Africa’, Volume 1, 2ndEdition, Royal Botanic Gardens, Kew. 1995.

Choi, J., Shendrik, I., Peacocke, M., Peehl, D., Buttyan, R., Ikeguchi, E.F., Katz, A.E. & Benson, M.C., 2000, ‘Expression of senescence associated beta-galactosidase in enlarged prostates from men with benign prostatic hyperplasia’, Urol. 56, 160-166.

Conserva, L.M. & Ferreira Júnior, J.C., 2012, ‘Borreria and Spermacoce species (Rubiaceae): A review of their ethnomedicinal properties, chemical constituents, and biological activities’, Pharmacogn. Rev. 6(11), 46-55.

Dacie, J.V. & Lewis, S.M., 1991, ‘Practical Haematology. Edingburgh: Churchhill Livingstone 7: 535-44.

Dhami, N., 2013. Trends in Pharmacognosy: a modern science of natural medicines. J. Herb. Med. 3(4), 123-131

Fu, S., Zhang, X., Niu, Y. & Wang, R.T., 2018, ‘Prostate specific antigen, mean platelet volume, and platelet distribution width in combination to discriminate prostate cancer from benign prostate hyperplasia’, Asian Pac. J. Cancer Prev. 19(3) 699-702

Güzel, A., 2021, ‘Can Preoperative Haematological parameters predict pathology results in patients undergoing open prostatectomy due to benign prostatic hyperplasia? A Retrospective Study. Meandros Med. Dent. J. 22, 47-52.

Ijioma, S.N., Osim, E.E., Nwankwo, A.A., Nwosu, C.O. & Ekeleme, C.M., 2019, ‘Antioxidant potentials and effects on the hematology and osmotic fragility scores of a polyherbal formulation used in Southeast Nigeria’, J. Basic. Clin. Physiol. Pharmacol. 30(4), 10.1515/jbcpp-2017-0099. https://doi.org/10.1515/jbcpp-2017-0099

Ikeyi, A.P., Okagu, I.U., Ezeanyika, L.U.S. & Alumanah, E.O., 2020, ‘Zapoteca portoricensis root crude methanol extract and its fractions normalises aberrations associated with benign prostatic hyperplasia in rats’, All Life. 13(1), 360-372.

Jiang, S., Song, C.S. & Chatterjee, B., 2019, ‘Stimulation of prostate cells by the senescence phenotype of epithelial and stromal cells: Implication for benign prostate hyperplasia’, FASEB, BioAdv. 1, 353-363.

Kajiwara, S., Ishii, K., Sasaki, T., Kato, M., Nishikawa, K., Kanda, H., Arima, K., Watanabe, M. & Sugimura, Y., 2020, ‘Castration-induced stromal remodelling disrupts the reconstituted prostate epithelial structure’, Lab. Invest. 100, 670-681.

La Vignera, S., Condorelli, R.A., Russo, G.I., Morgia, G. & Calogero, A.E. 2016, ‘Endocrine control of benign prostatic hyperplasia. Androl. 4(3), 404-411.

Liu, T.T., Thomas, S., Mclean, D.T., Roldan-Alzate, A., Hernando, D., Ricke, E.A. & Ricke, W.A., 2019. Prostate enlargement and altered urinary function are part of the aging process. Aging (Albany NY). 11(9), 2653-2669

Mbaka, G.O., Ogbonnia, S.O. & Olawunmi, O. 2013. The effects of ethanol extract of Raphia hookeri seed on exogenous testosterone and estradiol induced benign prostatic hyperplasia in adult male rats. J. Morphol. Sci. 30(4), 235–243.

Nwandu, C.J., Nyananyo, B.L. & Ozimede, C.O., 2019, ‘Phytochemical screening of leaf extracts of eleven selected tropical plants species from eastern and southern Nigeria’, J. Appl. SCI. Environ. Manag. 23(10), 1867-1873.

Ohkawa, H., Ohishi, N. & Yagi, K., 1979, ‘Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction’, Anal. Biochem, 95(2), 351-358.

Parrotta, J.A., 2001, ‘Healing Plants of Peninsular India. Wallingford’, pp.614-615. CAB International Publishing.

Shah, A., Shah, A.A., Nandakumar, K. & Lobo, R., 2021, ‘Mechanistic targets for BPH and prostate cancer: A review’, Environ. Health Rev. 36(2), 261-270.

Sharma, M., Chadha, R. & Dhingra, N., 2017, ‘Phytotherapeutic agents for benign prostatic hyperplasia: an overview’, Mini-Rev. Med. Chem. 17(14), 1346-1363.

Subramanya, M.D., Pai, S.R., Upadhya, V., Ankad, G.M., Bhagwat, S.S. & Hegde, H.V., 2015, ‘Total polyphenolic contents and in vitro antioxidant properties of eight Sida species from Western Ghats, India’, J. Ayurveda. Integr. Med. 6, 24‑8.

Taoka, R. & Kakehi, Y., 2017, ‘The influence of asymptomatic inflammatory prostatitis on the onset and progression of lower urinary tract symptoms in men with histologic benign prostatic hyperplasia’, Asian J. Urology. 4, 158-163.

Ugwu, M.N., Asuk, A.A., Eteng, M.U. & Emuru, E.O., 2019, ‘Evaluation of impact of some selected plants on haematological parameters of testosterone and estradiol induced benign prostatic hyperplasia adult male rats’, Int. Blood Res. Rev. 10(1), 1-15.

Uroko, R.I., Simeon, I.E., Oluomachi, N.U., Chidiogo, A.O., Amarachi, A. & Cosmos, C.A., 2018, ‘Therapeutic effects of methanolic extract of Funtumia africana leaves on antioxidants and hematological indices of carbon tetrachloride-induced oxidative stress in rats’, Drug Invent. Today. 12 (1),114-122.

Uroko, R.I., Ijioma, S.N., Ogwo, E.U. & Awah, F.M., 2022b, ‘Combined ethanol extract of Hypselodelphys poggeana and Spermacoce radiata leaves ameliorate benign prostatic hyperplasia in rats via modulation of serum sex hormonal levels’, J. Res. Pharm.; 26(4), 742-752.

Uroko, R.I., Nweje-Anyalowu, P.C., Aaron, C.F. & Chukwu, C.N. 2022a, ‘Combined extract of Spermacoce radiata (DC.) Hiern and Hypselodelphys poggeana (K.Schum.) Milne-Redh leaves (CEESH) confer hepatoprotection in rat induced benign prostatic hyperplasia’, Plant Biotechnol. Persa. 4(1), 78-88

Uroko RI, Nwuke CP, Egba SI, Uchenna ON, Nwaekwu, FE, Anyanwu, PO (2020) Effects of combined ethanolicic extracts of Funtumia Africana and Abutilon Mauritianum leaves on sex Hormones and prostate indices of benign prostatic Hyperplasia induced rats, Animal Research International 17(3):3892 – 3902

Ursini, F., Maiorino, M. & Gregolin, C., 1985, ‘The selenoenzyme phospholipid hydroperoxide glutathione peroxidase’, Biochim. Biophys. Acta. 839, 62-70.

Xin, Z., Waterman, D.F., Hemken, R.W. & Harmon, R.J., 1991, Effects of copper status on neutrophil function, superoxide dismutase, and copper distribution in steers’, J. Dairy Sci. 74, 3078-3085.

Zuo, L., Prather, E.R., Stetskiv, M., Garrison, D.E., Meade, J.R., Peace, T.I. & Zhou, T., 2019, ‘Inflammaging and oxidative stress in human diseases: From molecular mechanisms to novel treatments’, Int. J. Mol. Sci. 20(18), 4472.



DOI: https://doi.org/10.22146/mot.76400

Article Metrics

Abstract views : 901 | views : 817

Refbacks

  • There are currently no refbacks.




Copyright (c) 2022 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada