The Spectometric Quantification of Total Content of Flavonoid, Phenol, and Alkaloid in Kesum leaf (Polygonum minus Huds) from West Borneo with Methanol and Ethanol Solvent

https://doi.org/10.22146/mot.68497

Dian Kartikasari(1*), Ika Ristia Rahman(2), Heni Puspasari(3), Abduh Ridha(4)

(1) Akademi Farmasi Yarsi Pontianak, Pontianak, West Kalimantan
(2) Akademi Farmasi Yarsi Pontianak, Pontianak, West Kalimantan
(3) Akademi Farmasi Yarsi Pontianak, Pontianak, West Kalimantan
(4) Akademi Farmasi Yarsi Pontianak, Pontianak, West Kalimantan
(*) Corresponding Author

Abstract


Kesum (Polygonum minus Huds) is one of the typical plants that grow in West Borneo. The Kesum leaves are the part which is mostly used. Kesum leaves contain a lot of phytochemical compounds. This study quantitatively determined the total phenolic, flavonoid, and alkaloid contents in methanol and ethanolic extract of Polygonum minus Huds leaves using a spectrophotometric method. The samples were extracted by maceration. The content was determined by using a UV-Vis spectrophotometer. Based on the assay result, the methanol extract contained a total of phenolic 49.351±0.430 ppmEAG (equivalent to gallic acid); total of flavonoids 44.128±0.116ppmEQ (quercetin equivalent); total of alkaloids 356,798±1,004ppmEP (piperine equivalent). The 96% ethanol extract contained total of phenol 46,196±0.329ppmEAG; total of flavonoids 34,969±0.116ppmEQ; total of alkaloids 183.525±0.766ppmEP. The 70% ethanol extract contained total of phenol 64.189+0.215ppmEAG; total of flavonoids 27.834±0.158ppmEQ; total of alkaloids 432,947±1,533ppmEP. The ethanol extract 50% contained total of phenol 67,343±0.164ppmEAG; total of flavonoids 26.063±0.158ppmEQ; total of alkaloids 679,142±2,525ppmEP. All of the results are preliminary data for further research, especially for the isolation of compounds with specific targets or for utilization as main ingredients for traditional medicine.

Keywords


Polygonum minus Huds; phenols; flavonoids; alkaloids; different solvents

Full Text:

PDF


References

Ahmad, A., Husain, A., Mujeeb, M.,Khan, S. A., Alhadrami, H. A.A., & Bhandari, A. (2015). Quantification of Total Phenol, Flavonoid Content and Pharmacognostical Evaluation Including HPTLC Fingerprinting for The Standardization of Piper nigrum Linn Fruits. Asian Pasific Journal of Tropical Biomedicine, 5(2), 101–107.

Ahmad, I., Arifuddin, M., & Rijai, L. (2016). The effect of extraction methods of Bawang Dayak (Eleutherine palmifolia L. MERR) against TLC profiles and sunscreen activities. International Journal of PharmTech Research, 9(9), 428–436.

Ansel, H.C., 1989, Pengantar Bentuk Sediaan Farmasi, diterjemahkan oleh Farida Ibrahim, Asmanizar, Iis Aisyah, Edisi keempat, 255-271, 607-608, 700, Jakarta, UI Press.

Baba, S. A., & Malik, S. A. (2015). Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Taibah University for Science, 9(4), 449–454. https://doi.org/10.1016/j.jtusci.2014.11.001

Bobo-García, G., Davidov-Pardo, G.,Arroqui, C., Vírseda, P., MarínArroyo,M., & Navarro, M.(2014). Intra-laboratory validation of microplate methods for total phenolic content and antioxidant activity on polyphenolic extracts, and comparison with conventional spectrophotometric methods. Journal of the Science of Food and Agriculture, 95(1), 204–209. https://doi.org/10.1002/jsfa.6706

Dewi S, Asseggaf SNYRS, Natalia D, Mahyarudin, 2019, Efek Ekstrak Etanol Daun Kesum (Polygonum minus Huds.) sebagai Antifungi terhadap Trichophyton rubrum, Jurnal Kesehatan Andalas. 8(2), 199-204

Do, Q. D., Angkawijaya, A. E.,Tran-Nguyen, P. L., Huynh, L.H., Soetaredjo, F. E., Ismadji,S., & Ju, Y. H. (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22(3), 296–302.https://doi.org/10.1016/j.jfda.2013.11.001

Ghosh, M., Sinha, B. N., Seijas, J.A., Vázquez-Tato, M. P., & Feás, X. (2014). Flavonoids and Phenolic Compounds from <em>Litsea polyantha</em> Juss. bark. Proceedings of The 18thInternational Electronic Conference on Synthetic Organic Chemistry, b016. https://doi.org/10.3390/ecsoc18-b016

Globinmed. (2010). Retrieved Januari 5 2021, from Globinmed: http://www.globinmed.com/index.php?option=com_content&view=article&id=86112:hibiscus

Kartikasari D., Ika Ristia Rahman IR, Ridha A., 2021, Uji Fitokimia Dengan Beberapa Pelarut Pada Daun Kesum (Polygonum Minus Huds.) Dari Kalimantan Barat, Laporan Penelitian, Akademi Farmasi Yarsi Pontianak

Marinova, D., Ribarova, F., & Atanassova, M. (2005). Total phenolics and total flavonoids in bulgarian fruits and vegetables. Journal of the University of Chemical Technology and Metallurgy, 40(3), 255–260.

Maulidya DA., 2017, Daya Antelmintik Ekstrak Etanol Daun Kesum (Polygonum minus) terhadap Ascaridia galli secara in vitro, Jurnal Mahasiswa PSPD FK Universitas Tanjungpura, 3(1), 731-740

Purwaningsih I, Sapriani R, Indrawati R., 2018, Antioxidant Activity Of Methanol Extract Of Kesum Leaves (Polygonum Minus Huds.) Dpph Methodaktivitas Antioksidan Ekstrak Metanol Daun Kesum (Polygonum Minus Huds.) Metode Dpph, Jurnal Laboratorium Khatulistiwa, 1(2), 161-165

Sari, A. K., Alfian, R., Musiam, S.,Prasdianto, & Renny. (2018).Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Metanol Kayu Kuning (Arcangelisia flava Merr) dengan Metode Spektrofotometri UV-Visibel. Jurnal Insan Farmasi Indonesia, 1(2), 210–217.

Shamsa, F., Monsef, H., Ghamooshi, R., dan Verdian-rizi, M., 2008. Spectrofotometric Determination of Total Alkaloid in Some Iranian Medical Plants. Thai J.Pharm Sci, 32: 17-20

Sulianti S, Kuncari ES, Chairul SM. 2006, Pemeriksaan Farmakognosi dan Penapisan Fitokimia dari Daun dan Kulit Batang Calophyllum inophyllum dan Calophyllum soulatri. 7:25–9.



DOI: https://doi.org/10.22146/mot.68497

Article Metrics

Abstract views : 2859 | views : 2199

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2022 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada