.png)
.png)
* Corresponding Author
Effect of AGE and Gamma-mangostin on Luteinizing Hormone Receptor (LHR) Levels in Leydig Cell Culture of Male Aging Rats: Preliminary Study
https://doi.org/10.22146/mot.66827 Dicky Moch Rizal(1*), Muhammad Herdhana Ash Shidiqi(2), Rustamaji Rustamaji(3)
(1) Department of Physiology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta
(2) Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta
(3) Department of Pharmacology and Therapy, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author
Abstract
Advanced Glycation End Products (AGE) are proteins that can cause cell destruction by increasing oxidative stress and inflammation. This product resulted from a series of chemical reactions after an initial glycation reaction. A Leydig cell is one of the types of cells affected by AGE. This cell is located in the interstitial of the testes and stimulated by the luteinizing hormone. This study aimed to compare the luteinizing hormone receptor levels in Leydig cell culture of Sprague-Dawley rat induced by AGE only and the one that administered gamma-mangosteen. We conducted an experimental laboratory study on luteinizing hormone receptor levels in Leydig cell culture of Sprague-Dawley rats induced by advanced glycation end products 200 μg/mL and given gamma-mangostin 5 μM compared to the one that was not given gamma-mangostin. The highest mean of LHR level was in group 3 given gamma-mangostin 5 μM (8.06 pg/ cells/24h), and the lowest mean was in group 1 (control) (7.78 pg/ cells/24h). The LHR levels in the rats' Leydig cell culture given 5μM gamma-mangostin were higher than the other groups indicate the inhibition capacity on the oxidation process caused by AGE in aging rats Leydig cells culture.
Keywords
Full Text:
PDFReferences
Agarwal, A., & Said, T. M. (2005). Oxidative stress, DNA damage and apoptosis in male infertility: a clinical approach. BJU Int, 95(4), 503-507. doi:10.1111/j.1464-410X.2005.05328.x
Akao, Y., Nakagawa, Y., Iinuma, M., & Nozawa, Y. (2008). Anti-cancer effects of xanthones from pericarps of mangosteen. Int. J. Mol. Sci., 9(3), 355-370. doi:10.3390/ijms9030355
Baek, J. Y., Jung, K., Kim, Y.-M., Kim, H.-Y., Kang, K. S., & Chin, Y.-W. (2021). Protective Effect of γ-mangostin Isolated from the Peel of Garcinia mangostana against Glutamate-Induced Cytotoxicity in HT22 Hippocampal Neuronal Cells. Biomolecules, 11(2), 170. doi:10.3390/biom11020170
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif;
mso-fareast-font-family:"Times New Roman";color:black'>
field-begin'> ADDIN EN.REFLIST
style='mso-element:field-separator'>Bierhaus, A., Chevion, S., Chevion, M., Hofmann, M., Quehenberger, P., Illmer, T., . . . Nawroth, P. P. (1997). Advanced glycation end product-induced activation of NF-kappaB is suppressed by alpha-lipoic acid in cultured endothelial cells. Diabetes, 46(9), 1481-1490. doi:10.2337/diab.46.9.1481
Chang, H.-F., & Yang, L.-L. (2012). Gamma-Mangostin, a Micronutrient of Mangosteen Fruit, Induces Apoptosis in Human Colon Cancer Cells. Molecules, 17, 8010-8021. doi:10.3390/molecules17078010
Foley, P. L. (2005). Common Surgical Procedures in Rodents. In J. Reuter & M. Suckow (Eds.), Laboratory Animal Medicine and Management. USA: International Veterinary Information Service.
Goluža, T., Boscanin, A., Cvetko, J., Kozina, V., Kosović, M., Bernat, M. M., . . . Ježek, D. (2014). Macrophages and Leydig Cells in Testicular Biopsies of Azoospermic Men. Biomed Res Int, 2014, 828697. doi:10.1155/2014/828697
Jittiporn K, Moongkarndi P, Samer J, Kumphune S, & W, S. (2019). Water extract of mangosteen suppresses H2O2-induced endothelial apoptosis by inhibiting oxidative stress. J Appl Pharm Sci, 9(09), 10-16.
Lei, Z. M., Mishra, S., Zou, W., Xu, B., Foltz, M., Li, X., & Rao, C. V. (2001). Targeted disruption of luteinizing hormone/human chorionic gonadotropin receptor gene. Mol Endocrinol, 15(1), 184-200. doi:10.1210/mend.15.1.0586
Li, J., & Schmidt, A. M. (1997). Characterization and functional analysis of the promoter of RAGE, the receptor for advanced glycation end products. J Biol Chem, 272(26), 16498-16506. doi:10.1074/jbc.272.26.16498
Mallidis, C., Agbaje, I., Rogers, D., Glenn, J., McCullough, S., Atkinson, A. B., . . . McClure, N. (2007). Distribution of the receptor for advanced glycation end products in the human male reproductive tract: prevalence in men with diabetes mellitus. Hum Reprod, 22(8), 2169-2177. doi:10.1093/humrep/dem156
Ohno, R.-I., Moroishi, N., Sugawa, H., Maejima, K., Saigusa, M., Yamanaka, M., . . . Nagai, R. (2015). Mangosteen pericarp extract inhibits the formation of pentosidine and ameliorates skin elasticity. Journal of clinical biochemistry and nutrition, 57(1), 27-32. doi:10.3164/jcbn.15-13
Rasheed, Z., Akhtar, N., & Haqqi, T. M. (2011). Advanced glycation end products induce the expression of interleukin-6 and interleukin-8 by receptor for advanced glycation end product-mediated activation of mitogen-activated protein kinases and nuclear factor-κB in human osteoarthritis chondrocytes. Rheumatology 50(5), 838-851. doi:10.1093/rheumatology/keq380
Rizal, D. M., Fauzi, A. R., & Rustamaji. (2019). Effect of gamma-mangostin on testosterone levels in Leydig cell culture of Sprague-Dawley rat induced by advanced glycation end products: a preliminary study. BMC Proc, 13(11), 12. doi:10.1186/s12919-019-0173-x
Sharma, R. K., Pasqualotto, A. E., Nelson, D. R., Thomas, A. J., Jr., & Agarwal, A. (2001). Relationship between seminal white blood cell counts and oxidative stress in men treated at an infertility clinic. J Androl, 22(4), 575-583.
Singh, R., Barden, A., Mori, T., & Beilin, L. (2001). Advanced glycation end-products: a review. Diabetologia, 44(2), 129-146. doi:10.1007/s001250051591
Sun, J., Zhong, L., Zhu, Y., & Liu, G. (2011). Research on the Isolation of Mouse Leydig Cells Using Differential Digestion with a Low Concentration of Collagenase. J. Biol. Chem, 57(3), 433-436. doi:10.1262/jrd.10-123N
Sze Lim, Y., Sze Hui Lee, S., & Chin Tan, B. (2013). Antioxidant capacity and antibacterial activity of different parts of mangosteen (Garcinia mangostana Linn.) extracts. Fruits, 68(6), 483-489. doi:10.1051/fruits/2013088
Wang, Y., Chen, F., Ye, L., Zirkin, B., & Chen, H. (2017). Steroidogenesis in Leydig cells: effects of aging and environmental factors. Reproduction, 154(4), R111-r122. doi:10.1530/rep-17-0064
Zhang, F. P., Poutanen, M., Wilbertz, J., & Huhtaniemi, I. (2001). Normal prenatal but arrested postnatal sexual development of luteinizing hormone receptor knockout (LuRKO) mice. Mol Endocrinol, 15(1), 172-183. doi:10.1210/mend.15.1.0582
Zhao, Y.-T., Qi, Y.-W., Hu, C.-Y., Chen, S.-H., & Liu, Y. (2016). Advanced glycation end products inhibit testosterone secretion by rat Leydig cells by inducing oxidative stress and endoplasmic reticulum stress. Int J Mol Med, 38(2), 659-665. doi:10.3892/ijmm.2016.2645
Zirkin, B. R., & Papadopoulos, V. (2018). Leydig cells: formation, function, and regulation. Biology of reproduction, 99(1), 101-111. doi:10.1093/biolre/ioy059
%;font-family:"Times New Roman",serif;mso-fareast-font-family:"Times New Roman";
color:black;mso-ansi-language:EN-US;mso-fareast-language:EN-US;mso-bidi-language:
AR-SA'>
DOI: https://doi.org/10.22146/mot.66827
Article Metrics
Abstract views : 1485
| 
views : 1330
Refbacks
- There are currently no refbacks.
Copyright (c) 2021 Majalah Obat Tradisional
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Faculty of Pharmacy
Universitas Gadjah Mada