Application of Ozonated Olive Oil as Adjunctive Therapy after Periodontal Pocket Curettage towards Collagen Density of Alveolar Bone in Periodontitis Healing Process (In Vivo Study with Sprague dawley)

Dahlia Herawati(1*), Etty Cahya Pertiwi(2)

(1) Department of Periodontology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(2) Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author


Periodontitis is an inflammatory disease of the supporting tissue of the teeth caused by specific microorganisms or groups of specific microorganisms, resulting in progressive destruction of the periodontal ligament and alveolar bone with periodontal pocket formation. Application of ozonated olive oil in dentistry is based on the actions such as antimicrobial and therapeutic agent, needed as adjunctive therapy after periodontal pocket curretage. Collagen is the main constituent of alveolar bone extracellular matrix and needed as a scaffold in the formation of mineralized matrix. The aim of this study was to determine the density of collagen in alveolar bone on periodontitis healing process after adjunctive topical application of ozonated olive oil in periodontal pocket curretage. In this study, 32 Sprague dawley rats were randomly divided into two groups: curetted-topical application of ozonated olive oil as treatment groups and curetted-topical application of 1% CMC-Na as placebo group. Periodontitis induced by placing silk-ligature around submandibular incisors for 7 days. Subsequently, the rats were sacrificed on days 3, 5, 7, and 14 after curetted and topical application, and each group was represented by four rats. The staining was done using Mallory staining method. All the results were statistically analyzed using Kruskal-Wallis and Mann-Whitney tests. The results showed that there were significant differences (p<0,05) in the density of collagen between two groups. The study concluded that adjunctive topical application of ozonated olive oil after periodontal pocket curretage significantly increase the density of collagen in alveolar bone on periodontitis healing process in Spraque dawley.


periodontitis; curetted; ozonated olive oil; collagen; Sprague dawley

Full Text:



Ahmed, J., Binnal, A., Rajan, B., Denny, C., & Shenoy, N., 2013, Ozone Application in Dentistry: An Overview, J Exp Integr Med, 3(3), 171-176.

Andriani, I, Meiyanto, E., Suryono & Ana, I.D., 2020, The combination of carbonate hydroxyapatite and human β-defensin 3 to enhance collagen fibre density in periodontitis Sprague Dawley rats, Dent. J., 53(2), 76–80.

Aoki, A., Katia, M.S., Hisashi, W., & Isao I., 2004, Laser in Non Surgical Periodontal Therapy, Periodontology 2000, 36, 59-79.

Bocci, V., Borrelli, E., Travegli, V., & Zanardi, I., 2009, The Ozone Paradox: Ozoneis A Strong Oxidant as well as A Medical Drug, Med. Res. Rev., 29(4), 646-82.

Filippi, A., 2001, The Influence of Ozonised Water on the Epithelial Wound Healing Process in the Oral Cavity, International Ozone Association, 15th World Congress, London.

Flanangan, M., 2000, The Physiology of Wound Healing, Journal of Wound Care, 9(6), 299-300.

Franscino, A.V., Mantesso, A., Correa, L., & Deboni, M.C.Z., 2013, Aqueous-ozone irrigation of bone monocortical wounds in hyperglycemic rats, Acta Cirurgica Brasileira, 28(5), 327-333.

Fratzl, P., 2008, Collagen Structure and Mechanics, Springer, New York, 22-23.

Gopalakrishnan, S. & Parthiban, S., 2012, Ozone-A New Revolution in Dentistry, J. Bio. Innov., 1(3), 53-69.

Herawati, D., Anggraeni, D., & Damayanti, A.R., 2020, Effect of Ozonated Olive Oil in Topical Application towards osteoblast Number and Angiogenesis of Alveolar Bone in Periodontitis Healing Process In vivostudy in Sprague dawley Rats, Trad.Med.J., 25(1), 59-66.

Hernandez-Gill, I.F., Gracia, M.A.A., Pingarron, M.C., & Jerez, L.B, 2006, Physiological Bases of Bone Regeneration I. Histology and Physiology of Bone Tissue, Med. Oral. Patol. Oral Cir. Bucal, 11, 47-51.

Ismardianita, E., Widyawati, Elianora, D., Rosalina, W., Nofrike, L., Khairani, V.Y., 2020, The effectiveness methanol extract clausena excavate on number of fibroblast and density of collagen fibers after tooth extraction, Journal of Dentomaxillofacial Science, 4(3), 170-175.

Johnson, D.K. & Perez, M., 2010, Local Delivery of Chemotherapeutic Agents in Periodontal Therapy, Naval Postgraduated Dental School, National Naval Dental Center, Bethesda, Maryland, 15-17.

Kamal, N., 2010, Pengaruh Bahan Aditif CMC (Carboxyl Methyl Cellulose) terhadap Beberapa Parameter pada Larutan Sukrosa, Jurnal Teknologi, 1(17), 78-84.

Kiani, F.A., Kuchiwal, A.B., Shah, M.G., Khan, M.S., Lochi, G.M., Manan, A., ul HaQ, I., & Khan, F.M., 2014, Histological Characterization of Wound Healing of Flank verses Midline Ovariohyterectomy in Different Age Groups of Cats, J. of Clin. Path. and Fornsc, 2(2), 6-16.

Kim, H.S., Noh, S.U., Han, Y.W., Kim, K.M., Kang, H., Kim, H.O., & Park, Y.M., 2009, Therapeutic Effects of Topical Application of Ozone on Acute Cutaneous Wound Healing, J. Korean Med. Sci., 24(3), 68-74.

Kream, B.E. & Lichtler, A.C., 2011, Vitamin D Regulation of Type I Collagen Expression in Bone, in D. Feldman, J. W. Pike, and J. S. Adams (ed.): Vitamin D 3rd ed, pp. 403-404, Elsevier, USA.

Kumar, V. and Chandni, 2021, Ozone in Dentistry: A Review, Int Healthc Res J, 5(3), RV1-RV5.

Lubojanski, A., Dobrzynski, M., Nowak, N., Rewak-Soroczynska, J., Sztyler, K., Zakrzewski, W., Dobrzynski, W., Szymonowicz, M., Rybak, Z., Wiglusz, K., Wiglusz, R.J., 2021, Application of Selected Nanomaterials and Ozone in Modern Clinical Dentistry, Nanomaterials, 11, 259.

Mangano, A., Mangano, A., Lianos, G.D., Picone, M., & Dionigi, G., 2015, TGF-β Superfamily, Molecular Signaling and Biomimetic Features for Bone Regeneration: Historical Perspectives and Future Applications, Updates Surg., 67, 321-323.

Mosallam, R.S., Nemat, A., El-Hoshy, & Suzuki, S., 2011, Effect of Oleozon on Healing of Exposed Pulp Tissue, J. of Ame. Sci., 7(5), 38-44.

Newman, M.G., Takei, H.H., Klokkevold, P.R., & Carranza, F.A., 2012, Carranza’s Clinical Periodontology 11th Edition, Elsevier Saunders, Missouri, 154.

Polimeni, G., Xiropaidis, A.V., & Wikesjo, U.M.E., 2000, Biology and Principles of Periodontal Wound Healing/Regeneration, Periodontology, 41, 30-47.

Putra, A.S.S, Rahardjo, Hasan, C.Y & Pangestiningsih, T.W., 2021, Effect of Concentration Differences of Snail Mucus Gel (Achatina Fulica) on Collagen Density and Wound Closure Rate in Wistar Rat Skin Punch Biopsy Wounds, Journal of International Dental and Medical Research, Volume 14 Number 2, 574-579.

Re, L., Martinez-Sanchez, G., Perez-Davison, G., & Sirito, M., 2010, Role of Ozone/Oxygen in Fibroblast Growth Factor Activation. Discovering the Facts, Intl. J. of Ozone The., 9, 55-58.

Reeves, R., Ribeiro, A., Lombardo, L., Boyer, R., & Leach, J.B., 2010, Synthesis and Characterization of Carboxymethylcellulose-Methacrylate Hydrogel Cell Scaffolds, Polymers, 2, 252-264.

Sabirin, I.P.R., Maskoen, A.M., & Hernowo, B.S., 2013, Peran Ekstrak Etanol Topikal Daun Mengkudu (Morinda citrifolia L.) pada Penyembuhan Luka Ditinjau dari Imunoekspresi CD34 dan Kolagen pada Tikus Galur Wistar, MKB, 45(4), 226-233.

Schwartz, A. & Sanchez, G.M., 2012, Ozone Therapy and Its Scientific Foundations, ISCO3, Madrid, 11.

Shoukheba, M.Y.M. & Ali, Sh. A., 2014, The effects of subgingival application of ozonated olive oil gel in patient with localized aggressive periodontitis. A clinical and bacteriological study, Tanta Dental Journal, 11, 63-73.

Sudatri, N.W., 2010, Kadar Kolagen Kulit dan Tulang pada Tikus Betina Usia Enam dan 12 Bulan yang Disuplementasi dengan Somatotropin, Jurnal Biologi, XIV(1), 10-14.

Tandelilin, R.T.C., Safro, A.S.M., Santoso, A.L., Soesatyo, M.H.N.E., & Asmara,W., 2006, The Density of Collagen Fiber in Alveolus Mandibular Bone of Rabbit after Augmentation with Powder Demineralized Bone Matrix Post Incisivus Extraction, Dent. J., 39(2), 43-47.

Tjay, T.H. & Rahardja, K., 2007, Obat-obat Penting: Kasiat, Penggunaan dan Efek-efek Sampingnya Ed. 6, Elex Media Komputindo, Jakarta, 307.

Viguet-Carrin, S., Garnero, P., & Delmas, P.D., 2006, The Role of Collagen in Bone Strength, Osteoporos Int., 17, 319-336.

Xiao, W., Tang, H., Wu, M., Liao, Y., Li, K., Li, L., & Xu, X., 2017, Ozone oil promotes wound healing by increasing the migration of fibroblasts via PI3K/Akt/mTOR signaling pathway, Bioscience reports, 37(6).

Xu, X., Chen, H., Zhang, X., Zhai, Z., Liu, X., Qin, A., & Lu, E., 2014, Simvastatin Prevents Alveolar Bone Loss in an Experimental Rat Model of Periodontitis After Ovariectomy, J. of Transl. Med., 2, 284.


Article Metrics

Abstract views : 1734 | views : 2026


  • There are currently no refbacks.

Copyright (c) 2021 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada
View My Stats