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* Corresponding Author
The Effect of Red Betel Leaf (Piper crocatum) and Moringa Leaf Extracts on Endometritis Levels in Aceh Cows
https://doi.org/10.22146/mot.64626 Rosmaidar Rosmaidar(1), Meutia Handayani(2), Fadillah Fadillah(3), Teuku Armansyah(4), Tongku Nizwan Siregar(5*), Hafizuddin Hafizuddin(6), Husnurrizal Husnurrizal(7)
(1) Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh
(2) Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh
(3) Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh
(4) Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh
(5) Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh
(6) Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh
(7) Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh
(*) Corresponding Author
Abstract
This study aims to determine the ability of red betel leaf (Piper crocatum) or Moringa leaf extracts to reduce the endometritis level in Aceh cattle. In this study, six Aceh cows aged 3-5 years, weighing 150-250 kg from the Experimental Animal Technical Implementation Unit of Faculty of Veterinary Medicine of Syiah Kuala University were used. The cows were divided into two treatment groups, namely cows with endometritis that were given red betel leaf extract (T1) and cows with endometritis that were given Moringa leaf extract (T2). Examination of the endometritis levels was carried out before and after treatment using the White Side Test (WST) method. The collection of estrus cervical mucus was needed for the WST examination, and heat induction was performed with prostaglandin F2 alpha (PGF2α) at a dose of 25 μg. Collection of cervical mucus was performed 8-12 hours after the initiation of heat. All cows with endometritis were given intrauterine extracts of red betel leaves or Moringa leaves at a concentration of 20% every 24 hours for a week at a solution volume of 20 ml. The data were then analyzed using a paired t test. The mean endometritis levels before and after treatment on K1 vs. K2 were 3.0 and 1.7 vs. 2.7 and 2.7, respectively (P<0.05). It was concluded that red betel leaf extract at a concentration of 20% was more effective in reducing the endometritis level of Aceh cows than Moringa leaf extract.
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Achwandi, M., Azizah, K. & Soewito, 2015, ‘Efektivitas ekstrak daun sirih merah (Piper crocatum) terhadap kadar hambat minimum dan kadar bunuh minimum bakteri Salmonella typhi’, J. Nurs. Pract. 2, 1–8.
Ahmed, F.A., Saxena, M., & Ravikanth, K., 2014, ‘A herbal intrauterine infusion
“Uraksha Liquid” for treatment of reproductive disorders in cows’,
IJPRBS., 3, 42–48.
Ahmed, F.O. & Elsheikh, A.S., 2014, ‘Treatment of repeat breeding in dairy cows with lugols iodine’, IOSR J. Agricult. Vet. Sci. 7, 22–26..
Ahmed, F.O. & Elsheikh, A.S., 2014, ‘Treatment of repeat breeding in dairy cows with lugols iodine’, IOSR J. Agricult. Vet. Sci. 7, 22-26.
Alyasiri, E.A., Alwan, A.F., & Al-Hamedawi, T.M., 2015, ‘Comparative study
of some intrauterine treatment regimens on bacterial causes repeat breeders
in Iraqi buffaloes’, Papirex – Indian J. Res., 4, 188–190.
Amanda, S., Nyoman, M. & Gede, I.S., 2019, ‘Uji aktivitas antibakteri rebusan daun sirih (Piper betle Linn) terhadap bakteri Streptooccus pyogenes’. Meditory 7, 37–43.
Andjani, N., Sujuti, H. & Winarsih, S., 2016, ’Efek ekstrak etanol daun kelor (Moringa oleifera) terhadap nuclear factor kappa beta (Nf-kB) aktif dan apoptosis cell line kanker mcf-7’, Majalah Kesehatan FKUB. 3, 204–212.
Bedewy, R.B. & Rahawy, M.A., 2019, ‘Comparative study for detection subclinical endometritis in local cows’, Adv. Anim. Vet. Sci. 7, 289–294.
Bhardwaz, A., Nema, S.P., Mahour, S.S., Bagati, S. & Kumar, S., 2018, ‘Therapeutic efficacy of lugol’s iodine (I2KI) in infectious repeat breeder crossbred cows’, Int. J. Curr. Microbiol. Appl. Sci. 7, 648–654.
Bhat, F.A., Bhattacharrya, H.K. & Hussain, S.A., 2014, ‘White side test a simple and rapid test for evolution of nonspecific bacterial genital infectious of repeat breeding cattle’, Vet. Res. Forum, 5, 177–180.
Bina, F., Soleymani, T., Toliat, M., Hajimahmoodi, M., Tabarrai, M., Abdollahi, R. & Rahimi, 2019, ‘Plant-derived medicines for treatment of endometriosis: A comprehensive review molecular mechanisms’, Pharm. Res. 13, 76–90.
Brooks, G., Janet, S. B. & Stepen, A.M., 2005, ‘Mikrobiologi Kedokteran’, Edisi Pertama. Salemba Medika. Jakarta
Bukar, A., Uba, A. & Oyeyi, T.I., 2010, ‘Antimicrobial profile of Moringa oleifera extracts against some food-borne microorganisms’, Bayero J. Pure Appl. Sci., 3, 43–48.
Bustanussalam, Devi, A., Eka, S. & Dadang, J., 2015, ‘Efektivitas antibakteri ekstrak daun sirih (Pipper betle Linn) terhadap Staphylococcus aureus ATCC 25923’, Fitofarmaka, 5, 58–64.
Dima, L.L., Fatmawali, R.H. & Widya. A.L., 2016, ‘Uji aktivitas antibakteri ekstrak daun kelor (Moringa oleifera L) terhadap bakteri Escherichia coli dan Staphylococcus aureus’, J. Ilmiah Farmasi, 2, 282–289.
Ding, Z. & Lian F., 2015, ‘Traditional Chinese medical herbs stage therapy in infertile women with endometriosis a clinical study’, Int. J. Clin. Exp. Med., 8, 14085–14089.
Drillich, M., 2006, ‘An update on uterine infectious in dairy cattle’, Slov. Vet. Res,
43, 11–15.
Dwivedi, V. & Tripathi, S., 2014, ‘Review study on potential activity of Piper betel’, . J. Pharm. Phytochem. 3, 93–98.
Fitriah, Mappiratu, & Prismawiryanti, 2017, ‘Uji aktivitas antibakteri ekstrak daun tanaman johar (Cassia siamea Lamk.) dari beberapa tingkat kepolaran pelarut’, Kovalen. 3, 242–251.
Gupta, J.P., Shyma, K.P., Mohandas, A.C. & Sneharaj, R.K., 2011, ‘White side test to study subclinical endometritis in crossbred cattles’, Bhartiya Krishi Anushandhan Patrika. 26, 123–125.
Hafsari, A. & Pujiastutik, Y.E., 2018, ‘The potential effect of red dragon fruit (Hylocereus polyhizus) peel ethanol extract on endometriosis progressivity in endometriosis mice’ J. Kedokt. Hewan. 12, 53–56.
Hammon, D.S., Evjen, I.M., Dhiman, T.R., Goff, J.P. & Walters, J.L., 2006, ‘Neutrophil function and energy status in Holstein cows with uterine health disorders’, Vet. Immunol. Immunopathol. 113, 21–29.
Hasanah, H.M.M., Yahdi, & Yuli, K.D., 2020, ‘Studi komparasi kualitas dan daya antibakteri E. coli hand soap berbahan ekstrak daun sirih hijau (Piper betle Linn.), daun sirih merah (Piper crocatum), dan daun sirih cina (Peperomia pellucida)’ J. Kimia dan Pendidikan Kimia, 2, 191–209.
Heuwieser, W., Tenhagen, B.A., Tischer, M., Luhr, J. & Blum, H., 2000, ‘Effect of three programmes for the treatment of endometritis on the reproductive performance of a dairy herd’, Vet. Rec., 146, 338–341.
Hidayah, N., Aishyah, K.H., Ahmad, S., Irawati, & Dewi, M., 2016, ‘Uji efektivitas ekstrak Sargassum muticum sebagai alternatif obat bisul akibat aktivitas Staphylococcus aureus’, J Creativity Students, 1, 1–9.
Hussain, A.M. & Daniel, R.C.W., 1991, ‘Bovine endometritis: Current and future alternative therapy’, J. Vet. Med. Assoc., 38, 641–651.
Januarti, B.I., Wijayanti, R., Wahyuningsih, S. & Nisa, Z., 2019, ‘Potensi ekstrak terpurifikasi daun sirih merah (Piper crocatum Ruiz & Pav) sebagai antioksidan dan antibakteri’, J. Pharm. Sci. Clin. Res., 4, 60–68.
Lestari , Y. D., Hendarto, H., & Widjiati, 2018, ‘Pengaruh ekstrak etanol daun kelor (Moringa oleifera Lam) terhadap jumlah apoptosis sel granulosa pada mencit (Mus musculus) model endometriosis’, J. Biosains Pascasarjana, 1, 1–7.
Mandhwani, R., Anavil, B., Sudarshan, K., Madhu, S., & Ranjit, A., 2017, ‘Insight into bovine endometritis with special reference to phytotherapy;, Vet. World, 10, 1529–1532.
Martinez, J. & Thibier, M., 1984, ‘Reproductive disorders in dairy cattle: Respective influence of herds, seasons, milk yield and parity’, Theriogenology, 21, 569-581.
Maurya, S.N., Dabas, Y.P.S., & Gupta, R.S., 1992, ‘A note on bacteriological studies of cervical secretion of infertile cows and buffaloes’, Ind. J. Ani. Reprod. 13, 49–50.
Melia, J., Amrozi, & Tumbelaka, I.L., 2014, ‘Dinamika ovarium sapi endometritis yang diterapi dengan gentamisin, flumequin, dan analog prostaglandin f2 alpha (pgf2a) secara intra uterus’, J. Kedokt. Hewan, 8, 111–115.
Melia, J., Sadri, B., Siregar, T.N., Riady, G., Asmilia, N., Hanafiah, M., &
Panjaitan, B., 2020. ‘The effectiveness of lugol for endometritis therapy in
Aceh cow’, ICVAES, 19, 1-6
Mido, S., Murata, N., & Rawy, M.S., 2016, ‘Effects of intrauterine infusion of
povidone-iodine on endometrial cytology and bacteriology in dairy cows
with clinical endometriosis’, J. Vet. Med. Sci., 78: 551–556.
Moerfiah & Fira, D.S.S., 2011, ‘Pengaruh ekstrak daun sirih merah (Piper cf. fragile Benth.) terhadap bakteri penyebab sakit gigi’, Ekologia, 11, 30–35.
Nazzaro, F., Fratianni, F., Martino L.D., Coppola, R. & Feo, V.D., 2013. Effect of essential oils on pathogenic bacteria’, Pharmaceuticals, 6, 1451–1474.
Pratiwi, I. & Ira, S., 2012, ‘Efek ekstrak daun sirih merah (Piper crocatum Ruiz & Pav) terhadap pertumbuhan (Streptococcus pneumoniae)’, J. Ilmu Kesehatan dan Kedokteran Keluarga, 8, 1–5.
Prayoga, D.G.E., Komang, A.N. & Ni, N.P., 2019, ‘Identifikasi senyawa fitokimia dan aktivitas antioksidan ekstrak kasar daun pepe (Gymnema reticulum Br.) pada berbagai jenis pelarut’, J. Ilmu dan Teknologi Pangan, 8, 111–121.
Puspita, J.P., Safithri, M., & Sugiharti, N.P., 2018, ‘Aktivitas antibakteri ekstrak daun sirih merah (Piper crocatum)’, J. Curr. Biochem., 5, 1–10.
Putra, S.H.R., Surjowardojo, P., & Setyowati, E., 2017, ‘Pemanfaatan daun sirih merah (Piper crocatum) dalam menurunkan tingkat kejadian mastitis berdasarkan uji CMT dan SCC’, J. Ternak Tropika, 18, 22–28.
Rachmatiah, T., Vilya, S., & Lenggo, F., 2018, ‘Aktivitas daya hambat minyak atsiri dan ekstrak etanol daun sirih merah (Piper crocatum Ruiz & Pav.) terhadap Candida albicans’, Sainstech Farma, 11, 1–4.
Rafika, I., Thasmi, C.N., Herrialfian, Rosmaidar, & Hafizuddin, 2020, ‘Isolasi dan identifikasi bakteri gram negatif pada uterus sapi Aceh yang mengalami repeat breeding’, J. Agripet, 20, 187–192.
Retnaningsih, A., Ade, M.U. & Dewi, M.K., 2018, ‘Uji daya hambat antibakteri infusa daun sirih merah (Piper crocatum Ruiz & Pav) dan daun sirih hijau (Piper betle L) terhadap bakteri Staphylococcus aureus dengan metode difusi’, J. Analisis Farmasi, 3, 79–88
Reveny, J., 2011, ‘Daya antimikroba ekstrak dan fraksi daun sirih merah (Piper betle Linn.)’ J. Ilmu Dasar, 12, 6–12.
Rifai, G., I, Wayan R.W., & Komang, A.N., 2018, ‘Pengaruh jenis pelarut dan rasio bahan dengan pelarut terhadap kandungan senyawa fenolik dan aktivitas antioksidan ekstrak biji alpukat (Persea americana Mill.)’ J. ITEPA. 7, 22–32.
Rizkita, A. D., Edy, C., dan Sri, M. (2017). Isolasi dan uji antibakteri minyak daun sirih hijau dan merah terhadap Streptococcus mutans. Indonesians Journal of Chemical Science. 6(3) :1-8.
Sari, C. E., Hartono, M. & Surharyati, S., 2016, ‘Faktor-faktor yang mempengaruhi service per conception sapi perah pada peternakan rakyat di Provinsi Lampung’, J Ilmiah Peternakan Terpadu, 4, 313–318.
Scenzi, O., 2016, ‘Recent possibilities for diagnosis and treatment of post
parturient uterine disiases in dairy cow’, JFIV Reprod. Med. Genet., 4, 1–
7.
Sharma, P.S., Srivastava, R., Kumar, V.B. & Singh, P., 2018, ‘Phytoterapy Analternative low cost therapeutic management of endometritis in dairy animals: A review.’ Int. J. Curr. Microbiol. App. Sci., 14581-4591.
Sreemannarayana, O. & Rao, A.V.N., 1997, ‘A comparative study of infertility in crossbred cows and buffaloes under village management’, Indian. J. Anim. Reprod., 18, 46–47.
Suarez, M., Entenza, J.M., Doerries, C., Meyer, E., Bourquin, L., Sutherland, J., Marison, I., Moreillon, P., & Mermod, N., 2003, ‘Expression of a plant derived peptide harbouring water cleaning and antimicrobial activities’, Biotech. Bioeng. 81, 13–20.
Sudarwati & Sumarni, 2016, ‘Uji aktivitas senyawa antibakteri pada ekstrak daun kelor dan bunga rosella’, Indo. J. Chem. Sci. 5, 12–14.
Syafriana, V. & Rabitha, R., 2017, ‘Uji aktivitas antibakteri ekstrak etanol daun sirih merah (Piper crocatum) terhadap pertumbuhan Propionibacterium acnes’, Sainstech Farma., 10, 9–11.
Syahida, A. E.I., Prayitno, B.S., & Lusiastuti, M.A., 2013, ‘Pengaruh ekstrak daun sirih merah (Piper crocatum) terhadap profil darah dan kelulus hidupan ikan mas (Cyprinus carpio) yang diinfeksi bakteri Aeromonas hydrophila’, J. Aquacult. Management Technol., 2, 94–107.
Syahrinastiti, T.A., Aziz, D., & Lili, I., 2015, ‘Perbedaan daya hambat ekstrak daun sirih hijau (Piper betle L.) dan daun sirih merah (Piper crocatum Ruiz & Pav.) terhadap pertumbuhan Escherchia coli’, J Kesehatan Andalas, 4, 421–424.
Utami, D.E.R., Lutvia, K., & Yahdi, 2015, ‘Pengaruh jenis sirih dan variasi konsentrasi ekstrak terhadap pertumbuhan jamur Candida albicans’ J. Tadris IPA Biologi, 8, 142–156.
Verdiana, M.I., Wayan, R.W., & Dewa, G.M.P., 2018, ‘Pengaruh jenis pelarut pada ekstraksi menggunakan gelombang ultrasonik terhadap aktivitas antioksidan ekstrak kulit buah lemon (Citrus limon (Linn.) Burm F.)’, J Ilmu dan Teknologi Pangan, 7, 213–222.
Vifta, R.L., Muhammad, A.W., & Anita, K.H., 2017, ‘Perbandingan total rendemen dan skrining antibakteri ekstrak etanol daun sirih hijau (Piper betle L.) secara mikrodilusi’, J. Sci. Appl. Technol. 1, 87–93.
Waluyo, S.T., 2014,. Reproduksi Aplikatif pada Sapi. Srikandi Empat Wydia Utama, Bandung.
Wardani, D.V.K., Hendarto, H., & Widjiati, 2017, ‘Pengaruh ekstrak etanol daun kelor (Moringa oleifera Lam) terhadap jumlah sel mast pada mencit (Mus musculus) model endometriosis. J. Biosains Pascasarjana, 19, 1–6.
Yasa, I.G.D., Nengah, K.P., & Anak A.I.S.W., 2019, ‘Pengaruh konsentrasi etanol terhadap aktivitas antioksidan ekstrak daun sirih merah (Piper crocatum Ruiz & Pav.) menggunakan metode microwave assisted extraction (MAE)’, J. Ilmu Teknologi Pangan, 8, 278–284. DOI: https://doi.org/10.22146/mot.64626
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