.png)
.png)
* Corresponding Author
Hydroxyl Radical Scavenging Activity of Stachytarpheta jamaecensis Root Extract using In Vitro Deoxyribose Degradation Assay
https://doi.org/10.22146/mot.61746 Juliyatin Putri Utami(1*), Yusrinie Wasiaturrahmah(2), Deby Kania Tri Putri(3)
(1) Department of Biomedic, Faculty of Dentistry, University of Lambung Mangkurat
(2) Department of Biomedic, Faculty of Dentistry, University of Lambung Mangkurat
(3) Department of Biomedic, Faculty of Dentistry, University of Lambung Mangkurat
(*) Corresponding Author
Abstract
Use and demand of traditional medicinal plants currently growing in the direction that research in traditional medicines have also increased. One of the plants that is efficacious as a medicine is S. jamaecensis. This plant is usually used by people as a traditional medicine to treat various diseases. This study aims to determine the antioxidant activity of porterweed root. Antioxidant activity assays performed using hydroxyl radical scavenger that begins with the extraction by maceration. Dry roots of S. jamaecensis (250 g) was soaked with ethanol 96% for 3x24 hours at room temperature. The liquid extract obtained is evaporated by rotary evaporator and waterbath till viscous extracts is obtained. Then to extract performed phytochemical screening by quantitative and qualitative method. The antioxidant test was carried out using the hydroxyl radical scavenger method. The extract concentrations were varied, namely 125, 250, 500 and 1000 ppm. The data obtained was performed one-way ANOVA test. The results of the phytochemical screening showed the extract contains flavonoids, alcaloids, saponins, phenols and tannins. The results obtained were significant values from the antioxidant test with the antiradical scavenger method, the largest average value of root extract was 60.606% at a concentration of 1000 ppm. The results of spectrophotometric measurements showed that the extract has IC50 was 683.5294 µg/ml, while vitamin C has IC50 lower value (251.700 µg/ml).
Q
Keywords
Full Text:
PDFReferences
Ahmad, R., Munim, A., & Elya, B., 2012, Study of antioxidant activity with reduction of free radical DPPH and xanthine oxidase inhibitor of the extract Ruellia tuberosa Linn Leaf. International Research Journal of Pharmacy, 3(11).
Anggriawan, Muhamad Yuliet ,Khildah Khaerati., 2018, Pengaruh Pemberian Topikal Ekstrak Etanol Daun Pecut Kuda (Stachytarpheta jamaicensis (l.) Vahl) terhadap penyembuhan luka bakar derajat II pada punggung kelinci (Oryctolagus cuniculus). Biocelebes, Agustus, Volume 12 Nomor 2.
Ashraf, Mehdi Farshad., Maheran Abd Aziz, Johnson Stanslas, Ismanizan Ismail, and Mihdzar Abdul Kadir., 2013, Assessment of Antioxidant and Cytotoxicity Activities of Saponin and Crude Extracts of Chlorophytum borivilianum. The Scientific World Journal Volume, Article ID 216894, 7 pages. http://dx.doi.org/10.1155/2013/216894.
Asamari, A.M.; Addis, P.B.; Epley, R.J.; Krick, T.P, 1996, Wild rice hull antioxidants. Journal of Agriculture and Food Chemistry, 44, 126–130.
Borra SK, Mahendra J, Gurumurthy P, Jayamathi, Iqbal SS, Mahendra L., 2014, Effect of curcumin against oxidation of biomolecules by hydroxyl radicals, J Clin Diagn Res. 8(10):CC01-CC5. doi:10.7860/JCDR/2014/8517.4967
Chandrasekara, Anoma and Fereidoon Shahidi, 2018, Herbal beverages: Bioactive compounds and their role in disease risk reduction - A review. Journal of Traditional and Complementary Medicine, 8 (2018) 451e458.
Dina Pratiwi, Sri Wahdaningsih, Isnindar., 2013, The test of antioxidant activity from bawang mekah leaves (Eleutherine americana merr.) Using dpph (2,2- Diphenyl-1-picrylhydrazyl) method, Trad. Med. J.,18(1), p 9-16.
El Aziz MMA, Ashour AS, Melad ASG., 2019, A review on saponins from medicinal plants: chemistry, isolation, and determination, J Nanomed Res. 2019;8(1):282-288. DOI: 10.15406/jnmr.2019.07.00199.
Ghimire, Bhagirath., Geon Joon Lee, Sohail Mumtaz, and Eun Ha Choi, 2018, Scavenging effects of ascorbic acid and mannitol on hydroxyl radicals generated inside water by an atmospheric pressure plasma jet editors-pick. AIP Advances 8, 075021; https://doi.org/10.1063/1.5037125
GA Ayoola , HAB Coker, SA Adesegun , AA Adepoju-Bello , K Obaweya , EC Ezennia , TO Atangbayila, 2018, Phytochemical Screening and Antioxidant Activities of Some Selected Medicinal Plants Used for Malaria Therapy in Southwestern Nigeria., Tropical Journal of Pharmaceutical Research, September; 7 (3): 1019-1024.
Halliwell, B.; Aeschbach, R.; Loliger, J.; Aruoma, O.I, 1994, The characteristics of antioxidants. Food and Chemical Toxicology, 32, 671–683.
Harborne, J. B., 1987, Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Terbitan kedua Bandung : Penerbit ITB, Hal 70; 147-148; 243-235.
Iwu Irenus Chinonye , Onu Uchenna Lynda, Ukaoma Adanna A , Oze Rita .N., 2018, Phytochemical, Antimicrobial and Gc/Ms Analysis of the Root of Stachytarpheta Cayennensis (L .Vahl) Grown in Eastern Nigeria. International Research Journal of Natural Sciences Vol.6, No.2, pp.1-14, February
Jakub Treml and Karel ˇSmejkal., 2016, Flavonoids as Potent Scavengers of Hydroxyl Radicals. Comprehensive Reviews in Food Science and Food Safety Vol.15.
Khummueng. W., et al, 2020, Phytochemicals, Antioxidant and Cytotoxicity of Stachytarpheta jamaicensis (L.) Vahl Extracts. Thaksin University Journal, Vol.23(2) May - August.
Li X., 2013, Solvent effects and improvements in the deoxyribose degradation assay for hydroxyl radical-scavenging. Food Chem. 2013 Dec 1;141(3):2083-8. doi: 10.1016/j.foodchem.2013.05.084. Epub 2013 May 23. PMID: 23870931.
Li Y, Yu H, Jin Y, Li M, Qu C., 2018, Verbascoside Alleviates Atopic Dermatitis-Like Symptoms in Mice via Its Potent Anti-Inflammatory Effect, Int Arch Allergy Immunol. 175(4):220-230. doi: 10.1159/000486958. Epub, Mar 27. PMID: 29587260.
Liew, Pearl Majorie and Yoke Keong Yong., 2016, Stachytarpheta jamaicensis (L.) Vahl: From Traditional Usage to Pharmacological Evidence. Evidence-Based Complementary and Alternative Medicine, Volume, Article ID 7842340, 7 pages. http://dx.doi.org/10.1155/2016/7842340
Lipinski B. Hydroxyl radical and its scavengers in health and disease., 2011, Oxid Med Cell Longev. 2011:809696. doi: 10.1155/2011/809696. Epub 2011 Jul 17. PMID: 21904647; PMCID: PMC3166784.
M. Ganga Raju, S. Satyanarayana and K.Eswar Kumar., 2015, In vitro antioxidant assay of selected aqueous plant extracts and their polyherbal formulation. International Journal of Pharmacological Research. Volume 5 Issue 4. DOI:10.7439/ijpr IJPR.
Marliana, S. D., Suryanti, V. dan Suyono, 2005, Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis Komponen Kimia Buah Labu Siam (Sechium edule Jacq. Swartz.) dalam Ekstrak Etanol, Biofarmasi. Volume 3. Nomor 1, Hal 26-31.
Nafiu MO, Tom Ashafa AO., 2017, Antioxidant and inhibitory effects of saponin extracts from Dianthus basuticus Burtt Davy on key enzymes implicated in type 2 diabetes In vitro. Phcog Mag, 13, 576-82.
Ololade, OO., Ogunmola OO, Kuyooro SE, Abiona OO., 2017, Stachytarpheta jamaicensis leaf extract: Chemical composition, antioxidant, anti-arthritic, anti-inflammatory and bactericidal potentials, Journal of Scientific and Innovative Research. 6(4): 119-125.
Rojanasakul Y, Wang L, Hoffman AH, Shi X, Dalal NS, Banks DE, Ma JK., 1993, Mechanisms of hydroxyl free radical-induced cellular injury and calcium overloading in alveolar macrophages. Am J Respir Cell Mol Biol. Apr;8(4):377-83. doi: 10.1165/ajrcmb/8.4.377. PMID: 8386534.
Schapoval, E. et al., 1889, “Antiinflammatory and antinociceptive activities of extracts and isolated compounds from Stachytarpheta cayennensis.” Journal of ethnopharmacology 60 1, 53-9 .
Singh, D., & Chaudhuri, P. K., 2018, Structural characteristics, bioavailability and cardioprotective potential of saponins, Integrative medicine research, 7(1), 33–43. https://doi.org/10.1016/j.imr.2018.01.003
Sri Wahdaningsih, Erna Prawita Setyowati, Subagus Wahyuono, 2011, FREE RADICAL SCAVENGING ACTIVITY OF (Alsophila glauca J. Sm). Majalah Obat Tradisional, 16(3), 156 – 160, 2011.
Suaib Luqman & Ritesh Kumar., 2012, Importance of Deoxyribose Degradation Assay for Evaluating Hydroxyl Radical Scavenging Activity of Punica Extract, International Journal of Food Properties, 15:5, 942-948, DOI: 10.1080/10942912.2010.511753
Suthanthiran, Manikkam. Scott D. Solomon, Perry S. Williams, Albert L. Rubin, Abraham Novogrodsky & Kurt H. Stenzel, 1984, Hydroxyl radical scavengers inhibit human natural killer cell activity. Nature, volume 307, pages276–278(1984).
Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, dan Telser J, 2007, Free radicals and antioxidants in normal physiological functions and human disease. Inter J Biochem & Cell Biol. 39:44-84.
Wachidah, Leliana Nurul, 2013, Uji Aktivitas Antioksidan Serta Penentuan Kandungan Fenolat Dan Flavonoid Total Dari Buah Parijoto (Medinilla speciosa Blume). Jakarta : UIN Syarif Hidayatullah Jakarta
Xu, Dong-Ping, Ya Li, Xiao Meng, Tong Zhou, Yue Zhou, Jie Zheng, Jiao-Jiao Zhang and Hua-Bin Li., 2017. Natural Antioxidants in Foods and Medicinal Plants: Extraction, Assessment and Resources. Int. J. Mol. Sci. 2017, 18, 96; doi:10.3390/ijms1801009
DOI: https://doi.org/10.22146/mot.61746
Article Metrics
Abstract views : 2286
| 
views : 2012
Refbacks
- There are currently no refbacks.
Copyright (c) 2021 Majalah Obat Tradisional
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Faculty of Pharmacy
Universitas Gadjah Mada