Loading and Release Profile Assay of Carbonated Hydroxyapatite Incorporated with Propolis as Bone Graft Material
Indi Kusumawati(1), Suryono Suryono(2*), Ahmad Syaify(3)
(1) Clinical Dentistry Program, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Periodontology Department, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Periodontology Department, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author
Abstract
Periodontitis can lead to the destruction of the alveolar bone. The loss of the alveolar bone can be treated using carbonated hydroxyapatite (CHA) as a bone graft material. However, CHA is an alloplastic graft whose primary function is to act as a scaffold, but it is unable to stimulate the process of bone regeneration. Carbonated hydroxyapatite is an avascular synthetic material, which will increase the risk of bacterial adhesion on site that can lead to unsuccessful periodontal therapy. The incorporation of propolis into CHA is expected to add antibacterial capability into CHA. Besides its antibacterial property, propolis also has a bone regenerating effect. Mixing CHA with propolis needs to consider the process of loading the active ingredients into the carrier. The release of propolis is expected to occur gradually over a lengthy period. The purpose of this study was to analyze the loading and releasing assay for propolis incorporated with CHA. A propolis solution of 5%, 7.5%, and 10% was each incorporated into 10 mg of CHA. The loading percentage and releasing assay of propolis were measured. The absorbance reading was done at 289 nm using a UV-vis. It was shown that a 10% propolis solution had the highest loading percentage (32.08%), while the 5% propolis solution had the smallest loading percentage (10.63%). The propolis releasing profiles in all concentration groups were similar. The difference in propolis concentration incorporated with CHA affected the loading percentage but did not affect the propolis releasing assay.
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Ana, I.D., Matsuya, S. & Ishikawa, K. 2010. 'Engineering of carbonate apatite bone substitute based on composition-transformation of gypsum and calcium hydroxide', Engineering, 02(05), 344–352.
Anagnostakos, K. & Schrder, K. 2012. 'Antibiotic-impregnated bone grafts in orthopaedic and trauma surgery: A systematic review of the literature'. International Journal of Biomaterials,
Ardhani, R., Setyaningsih, Hafiyyah, O.A. & Ana, I.D. 2016. 'Preparation of carbonated apatite membrane as metronidazole delivery system for periodontal application'. Key Engineering Materials, 696, 250–258.
Bara, J.J. 2018. 'A doxycycline inducible, adenoviral bone morphogenetic protein-2 gene delivery system to bone'. Journal of Tissue Engineering and Regenerative Medicine, 12(1), e106–e118.
Bashutski, J.D. & Wang, H.L. 2009. 'Periodontal and endodontic regeneration'. Journal of Endodontics, 35(3), 321–328.
Cairo, R., Tomaz, R., Santos, W., Rago, S. & Rodrigues, V. 2006. 'Periodontitis treatment with Brazilian green propolis gel'. Pharmacology Online, 3(May), 336–341. http://pharmacologyonline.silae.it/files/archives/2006/vol3/026.Santos.pdf
Newman, M. G., Takei, H., Klokkevold, P. R. & Carranza, F.A. 2012. Carranza’s Clinical Periodontologi (11th ed.), Saunders Elsevier, St Louis Missouri.
Dashti, A., et al., (2010). 'In vitro antibacterial efficacy of tetracycline hydrochloride adsorbed onto Bio-Oss® bone graft'. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 93(2), 394–400.
Filho, H.N. et al., 2014. 'Autogenous bone grafts contamination after exposure to the oral cavity'. Journal of Craniofacial Surgery, 25(2), 412–414.
Madhumathi, K. & Kumar, T.S.S. 2014. ‘Regenerative potential and anti-bacterial activity of tetracycline loaded apatitic nanocarriers for the treatment of periodontitis’, Biomedical Materials. IOP Publishing, 9(3), p. 35002.
Peña, C., de la Caba, K., Eceiza, A., Ruseckaite, R. & Mondragon, I. 2010. 'Enhancing water repellence and mechanical properties of gelatin films by tannin addition'. Bioresource Technology, 101(17), 6836–6842.
Phaechamud, T. & Darunkaisorn, W. 2016. 'Drug release behavior of polymeric matrix filled in capsule'. Saudi Pharmaceutical Journal, 24(6), 627–634.
Samad, R., Akbar, F.H., Nursyamsi, N. & Awing, M. 2017. 'Propolis trigona sp. mouthwash efectiveness in lowering anaerobic gram-negative bacteria colonies', in Health Science International Conference (HSIC 2017), Atlantis Press., pp. 181–187 doi:
Scatolini, A.M., Pugine, S.M.P., De Oliveira Vercik, L.C., De Melo, M.P., & Da Silva Rigo, E.C. 2018. 'Evaluation of the antimicrobial activity and cytotoxic effect of hydroxyapatite containing Brazilian propolis'. Biomedical Materials (Bristol), 13(2), 1–31.
Suryono, S., Hasmy, N.S., Pertiwi, T.L. & Benyamin, B. 2017. 'Propolis 10%-gel as a topical drug candidate on gingivitis'. International Journal of Medicine and Pharmacy, 5(2), 12–17.
Zohery, A.A., Meshri, S.M., Madi, M.I., Abd El Rehim, S.S. & Nour, Z.M. 2018. 'Egyptian propolis compared to nanohydroxyapatite graft in the treatment of Class II furcation defects in dogs'. Journal of Periodontology, 89(11), 1340–1350.
DOI: https://doi.org/10.22146/mot.59226
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