Sunscreen Activity of Fraction n-hexane, Chloroform, and Eethyl Acetate of Ethanol 96% Flamboyan Leaf (Delonix regia. Raf) Extract

https://doi.org/10.22146/mot.54425

Putra Jiwamurwa Pama Tjitda(1*), Febri Odel Nitbani(2), Maria Kristina Bangko(3)

(1) Department of Pharmacy, Health Polytechnic Kemenkes Kupang
(2) Department of Chemistry, Faculty of Science and Engineering, Nusa Cendana University
(3) Department of Pharmacy, Health Polytechnic Kemenkes Kupang
(*) Corresponding Author

Abstract


The sunscreen test of fraction n-hexane, chloroform, and ethyl acetate of ethanol 96% flamboyant leaf (Delonix regia. Raf) extract had been performed. This research begins the extraction of a flamboyant leaf using 96% ethanol. Extraction used the maceration method. The extract is fractionation with some solvents such as n-hexane, chloroform, and ethyl acetate. Each fraction was identified secondary metabolite and sunscreen test involving SPF, %Te, and %Tp measurement using UV-Vis Spectrophotometer. The result of phytochemical screening exhibited flavonoids, alkaloids, saponins, tannins, and phenolics in ethyl acetate fraction. n-hexane and chloroform fraction don't show saponins and flavonoid content. The sunscreen test shows that chloroform fraction has good protection power toward UV light with SPF, %Te, and %Tp value is 54.27±0.462, 7.46±0.473, and 12.83±0.047 in 250 mg/L, respectively.


Keywords


Flamboyant leaf (Delonix regia. Raf); Sunscreen; Maceration

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DOI: https://doi.org/10.22146/mot.54425

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