Influence of Some Extraction Conditions Factor on Phenolic Content and Antioxidant Activity of Solanum betaceum Cav.

https://doi.org/10.22146/mot.51772

Erna Prawita Setyowati(1*), Andayana Puspitasari(2), Dias Insan Afini(3), Farida Hanum Nasution(4), Rosyidatun Nafingah(5)

(1) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) 1 Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia 2 Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
(4) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
(5) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


Solanum betaceum Cav. fruit is renowned for having antioxidant activity because it contains phenolic compounds. This study aimed to determine the effect of some condition factor namely solvent composition, maceration time, liquid-solid ratio, and the particle size of S. betaceum Cav fruit to the total phenolic content and antioxidant activity. The fruit was collected from Temanggung, Wonosobo, and Kopeng, Central Java, Indonesia. The research used single-factor experiments and simplex lattice design (SLD) as an optimization method.  Total phenolic content was determined using Folin Ciocalteau reagent, while antioxidant activity was determined using 2,2-diphenyl-1-picrilhidrazil (DPPH) radical scavenger activity. The solvent combination which gave the highest responses was ethanol: water (60:40 v/v) with phenolic content of 7.48% w/w EAG. Maceration for 8 hours will produce an extract with the highest total phenolic content (8.76% w/w EAG). The optimal solvent ratio was at 10:1 v/w with total phenolic content of 7.26 ± 0.20% w/w EAG. The optimum particle size was 600-850 μm with a total phenolic content of 6.07 ± 0.18% w/w EAG. Antioxidant activity with the DPPH free radical scavenger capture method from three regions did not show significant results.


Keywords


antioxidant activity; extraction conditions factor; Solanum betaceum Cav.; Temanggung-Wonosobo-Kopeng

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References

Acosta-Quezada, P. G., Raigón, M. D., Riofrío-Cuenca, T., García-Martínez, M. D., Plazas, M., Burneo, J. I., Figueroa, J. G., Vilanova, S., & Prohens, J., 2014, Diversity for Chemical Composition in a Collection of Different Varietal Types of Tree Tomato (Solanum betaceum Cav.), an Andean Exotic Fruit, Food chemistry, 169, 327-335.

Acosta, QPG., Raigón M.D., Riofrío, C.T., García, M.M.D., Plazas, M., Burneo, J.L., 2015, Diversity for chemical composition in a collection of different varietal types of tree tomato (Solanum betaceum Cav.), an Andean exotic fruit, Food Chem, 169:327–335.

Afini, D.I., 2017, Optimalisasi Rasio Pelarut-Simplisia Dan Ukuran Simplisia Terhadap Kadar Fenolik Total Pada Ekstraksi Buah Terong Belanda (Solanum Betaceum Cav.), Undergraduate Thesis, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Brewer, M.S., 2011, Natural Antioxidants: Sources, Compounds, Mechanisms of Action, and Potential Applications, Comprehensive reviews in Food Science and Food Safety, 10, 221-247

Chen, Y., Xie, M.Y., and Gong, X.F., 2007, Microwave-assisted extraction used for the isolation of total triterpenoid saponins from Ganoderma atrum, Journal of Food Engineering, 81: 162-170.

Chun, O. K., Kim, D., & Lee, C. Y., 2003, Superoxide Radical Scavenging Activity of the Major Polyphenols in Fresh Plums, J.Agric.Food.Chem, 51(27), 8067-8072.

Dai, J., and Mumper, RJ., 2010, Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties, Molecules, 15, 7313-7352.

Espin, S., Manzano, S.G., Taco, V., Poveda, C., Duran, B.A., Paramas, A.M.G., Buelga, C.S., 2016, Phenolic Composition and Antioxidant Capacity of Yellow and Purple-red Ecuadorian Cultivars of Tree Tomato (Solanum betaceum Cav.), Food. Chem. 194,1073-1080.

Luthria, DL., Mukhopadhyay, S., Krizek, DT., 2006, Content of total phenolics and phenolic acids in tomato (Lycopersicon esculentum Mill.) fruits as influenced by cultivar and solar UV radiation, Journal of Food Composition and Analysis, 19, 771–777

Hanum, F., 2017, Optimasi Komposisi Pelarut Dan Waktu Maserasi Terhadap Kadar Fenolik Total Ekstrak Buah Kemar (Solanum Betaceum Cav.), Undergraduate Thesis, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Kadir, N.A.A.A., Rahmat, A., and Jaafar, H.Z.E., 2015, Protective Effects of Tamarillo (Cyphomandra betacea) Extract against High Fat Diet Induced Obesity in Sparague-Dawley Rats, Journal of Obesity, 2015: 1-8.

Kedare, SB and Singh, RP., 2011, Genesis and development of DPPH method of antioxidant assay, J Food Sci Tchnol, 48 (4): 412-422

Magrone, T., Fontana, S., Laforgia, F., Dragone, T., Jirillo, E., Passantino, L., 2016, Administration of a Polyphenol-Enriched Feed to Farmed Sea Bass (Dicentrarchus labrax L.) Modulates Intestinal and Spleen Immune Responses, Oxid. Med. Cell. Longev, 216, 1–11.

Nafingah, R., 2017, Uji Aktivitas Penangkapan Radikal DPPH (2,2-difenil-1-pikrilhidrazil) dan Penetapan Kadar Fenolik Total Ekstrak Etanolik Buah Terong Belanda (Solanum betaceum Cav.) dan Fraksinya, Undergraduate Thesis, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Nakamura, M., Ra, JH., Ju, YJ., and Kim, SK., 2017,

Impact of different partitioned solvents on chemical composition and bioavailability of Sasa quelpaertensis Nakai leaf extract, Journal of Food and Drug Analysis, 25 (2), 316-326.

Orqueda, E., Rivas, M., Zampini, I.C., Alberto., R.A., Torres, S., Cuello, S., Sayago., J., Valdes, S.T., Aspee, F.J., Hirschmann, G.S., and Ineslsla, 2017, Chemical and functional characterization of seed, pulp and skin powder from chilto (Solanum betaceum), an Argentine native fruit. Phenolic fractions affect key enzymes involved in metabolic syndrome and oxidative stress, Food chemistry, 216 (70-79)

Prior, R. L., Wu, X., & Schaich, K., 2005, Standardized Methods for the Determination of Antioxidant Capacity and Phenolics in Foods and Dietary Supplements, Journal of Agricultural and Food Chemistry, 53(10), 4290-4302.

Safaralie, A., Fatemi, S., and Salimi, A., 2009, Experimental Design on Supercritical Extraction of Essential Oil from Valerian Roots and Study Optimal Conditions, Food and Bioproducts Processing, 88, 312-318.

Santangelo, C., Varì, R., Scazzocchio, B., Di Benedetto, R., Filesi, C., Masella, R., 2007, Polyphenols, intracellular signalling and inflammation, Ann.-Ist. Super. Sanita, 43: 394.

Sari, F., and Velioglu, Y.S., 2011, Effects of Particle Size, Extraction time and Temperature, and Derivatization Time on Determination of Theanine in Tea, Journal of Food Composition and Analysis, 24, 1130-1135

Spigno, G., Tramelli, L., & De Faveri, D. M., 2007, Effects of Extraction Time, Temperature and Solvent on Concentration and Antioxidant Activity of Grape Marc Phenolics, Journal of Food Engineering, 81(1), 200-208.

Suh, D.H., Lee, S., Heo, D.Y., Kim, Y.-S., Cho, S.K., Lee, S., dkk., 2014. Metabolite Profiling of Red and White Pitayas (Hylocereus polyrhizus and Hylocereus undatus) for Comparing Betalain Biosynthesis and Antioxidant Activity. Journal of Agricultural and Food Chemistry, 62: 8764–8771.

Thavamonev, T., Sivanadian, L., Tee, LH., Khoo, HE and Prasad, KN., 2012, Extraction and recovery of phytochemical components and antioxidative properties in fruit parts of Dacryodes rostrata influenced by different solvents, J Food Sci Technol, 55(7): 2523–2532

Vermerris, W., and Nicholson, R., 2006, Phenolic Compound Biochemistry, 1-2, Springer, The Netherlands.

Wong, BY., Tan, CP., and Ho, CW., 2013, Effect of solid-to-solvent ratio on phenolic content and antioxidant capacities of “Dukung Anak” (Phyllanthus niruri), International Food Research Journal, 20(1), 325-330

Wu, J., Yu, D., Sun, H., Zhang, Y., Wenwei, Z., Meng, F., and Du, X., 2015, Optimizing the Extraction of Antitumor Alkaloids from the Stem of Berberies amurensis by Response Surface Methodology, Industrial Crops and Products, 69, 68-75.



DOI: https://doi.org/10.22146/mot.51772

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