The Combination of Black Soybean Tempeh and Purple Sweet Potato Affect Reactive Oxygen Species and Malondialdehyde Level in Streptozotocin-Induced Diabetic Rats

https://doi.org/10.22146/mot.51544

Eka Pratama Putri(1), Sri Rahayu Lestari(2), Abdul Gofur(3*)

(1) Master Program of Biology, Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, East Java, Indonesia
(2) Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, East Java, Indonesia
(3) Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, East Java, Indonesia
(*) Corresponding Author

Abstract


Hyperglycemia conditions increase free radicals in the body that cause oxidative stress. Oxidative stress increase lipid peroxidation activity and reactive oxygen species (ROS). An antioxidant can prevent a free radical movement. The materials that contain potent antioxidants are black soybeans tempeh (BST) and purple sweet potatoes (PSP). The antioxidants in the BST are isoflavones with their derivates, and PSP is anthocyanins. This study aimed to determine the effect of BST and PSP extract on reactive oxygen species (ROS) and malondialdehyde (MDA) levels. In this study, rats were given a high-fat diet, 10% sucrose drink, and injected with multiple low-dose streptozotocin to induce T2DM. The animal's experiment divided into six groups: healthy rats, DM rats, DM rats + glibenclamide, DM rats + combination of BST and PSP in 3:1, 1:1, and 1:3 respectively. ROS levels were determined using the ELISA method and MDA levels were determined using spectrophotometer according to Thiobarbituric Acid (TBA) method. Our result suggests that the combination of BST and PSP significantly reduces ROS and MDA levels.

Keywords


free radical; purple sweet potatoes; T2DM; tempeh

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References

Baynest, H. W. Classification, Pathophysiology, Diagnosis, and Management of Diabetes Mellitus. Journal of Diabetes & Metabolism (2015), 06(05).

Ozougwu, J. C., Obimba, K. C., Belonwu, C. D., dan Unakalamba, C. B. The pathogenesis and pathophysiology of type 1 and type 2 diabetes mellitus. Journal of Physiology and Pathophysiology. (2013) Vol 4: pp. 46-57

Cho, N. H., Shaw, J. E., Karuranga, S., Huang, Y., da Rocha Fernandes, J. D., Ohlrogge, A. W., & Malanda, B. IDF Diabetes Atlas: Global estimates of diabetes prevalence for 2017 and projections for 2045. Diabetes Research and Clinical Practice (2018), vol.138, pp. 271–281.

Jung, C.-H., & Choi, K. M. (2017). Impact of High-Carbohydrate Diet on Metabolic Parameters in Patients with Type 2 Diabetes. Journal Nutrients (2017), vol. 9(4), pp.322.

Asmat, U., Abad, K., & Ismail, K. Diabetes mellitus and oxidative stress—A concise review. Saudi Pharmaceutical Journal (2016),24(5), 547–553

Sakuraba, H., Mizukami, H., Yagihashi, N., Wada, R., Hanyu, C., Yagihashi, S. Reduced beta-cell mass and expression of oxidative stress-related DNA damage in the islets of Japanese Type II diabetic patients. Diabetologia (2002) Vol 45: pp. 85-9

Pasaoglu, H., Sancak. B., Bukan, N. 2004. Lipid peroxidation in patients with type 2 diabetes mellitus. Tohoku J Exp Med Vol 004;203: 211-8.

Lobo, V., Patil, A., Phatak, A., & Chandra, N. Free radicals, antioxidants, and functional foods: Impact on human health. Pharmacognosy Reviews (2010), 4(8),

Rufer, C.E. & Kulling, S.E. 2006. Antioxidant Activity of Isoflavones and Their Major Metabolites Using Different in Vitro Assays. Journal of Agricultural and Food Chemistry, 54(8): 2926-2931.

Esaki, H., Ozaki, H., Kawakishi, S. & Osawa, T. New antioxidant isolated from tempeh. Journal of Agricultural and Food Chemistry (1996). Vol 44: pp.696–700

Gras, C. C., Nemetz, N., Carle, R., & Schweiggert, R. M. (2017). Anthocyanins from purple sweet potato ( Ipomoea batatas (L.) Lam.) and their color modulation by the addition of phenolic acids and food-grade phenolic plant extracts. Food Chemistry (2017), vol 235, pp. 265–274

Castro-Acosta, M. L., Smith, L., Miller, R. J., McCarthy, D. I., Farrimond, J. A., & Hall, W. L. Drinks are containing anthocyanin-rich blackcurrant extract decrease postprandial blood glucose, insulin, and incretin concentrations. The Journal of Nutritional Biochemistry (2016), Vol. 38, pp.154-161.

Gofur, A & Lestari S.R. (2016). Effect of black soybean natto extract (Glycine soja) on reproduction system of hypercholesterolemiamale mice. Asian Pacific Journal of Reproduction 2016; 5(5): 387–390

Batra.P & Sharma, A.K. 2013. Anti-cancer potential of flavonoids: recent trends and future perspectives. 3 Biotech (2013) 3:439–459

Gofur, A., Arifah S.N.,Annisa Y., Witjoro, A., Atho’illah M.F., Lestari S.R. (2019). Th Role of Black Soybean and Purple Sweet Potato Active Compound on Advanced Glycation End-Product in Streptozotocin-Induced Type 2Diabetes Mellitus Rat. Journal Of Tropical Life Science, Vol. 9, No. 2, 139 – 145

Astuti, M., Meliala, A., Dalais, F. S., & Wahlqvist, M. L. Tempe, nutritious, and healthy food from Indonesia. Asia Pacific Journal of Clinical Nutrition (2000), 9(4), 322–325

Ruffer CE, Kulling SE. 2006. Antioxidant activity of isoflavones and their major metabolites using different in vitro assays. J Agric Food Chem.54:2926–2931

Ji, Hua., Zhang, Haixin., Li, Hongtao,, & Li, Yonchao.. Analysis of the nutrition composition and antioxidant activity of different type of sweet potato cultivars. (2015). Food and Nutrition Sciences, 6, 161-167

Kasote, D., Katyare, S., Hedge, M & Bae, H. Significance of Antioxidant Potential of Plants and its Relevance to Therapeutic Applications. International Journal of Biological Sciences (2015), 11(8): 982-991

Yuan D, Yingni PAN, Yan Chen, Toshio Uno, Shahui Zhang, Yoshihiro Kano. An improved method for basic hydrolysis of isoflavone malonylglucosides and quality evaluation of Chinese soy materials. Chem. Pharm. Bull (2008), vol 56(1), pp. 1-6.

Ahmad, G., Almasry, M., Dhillon, A. S., Abuayyash, M. M., Kothandaraman, N., & Cakar, Z. Overview and Sources of Reactive Oxygen Species (ROS) in the Reproductive System. Oxidative Stress in Human Reproduction (2017), Pp. 1–16

Halliwell, B. Reactive Species, and Antioxidants. Redox Biology Is a Fundamental Theme of Aerobic Life. American Society of Plant Biologists (2006). Vol. 141, pp. 312–322

Procházková a, I. Boušová b, N. Wilhelmová. Antioxidant and prooxidant properties of flavonoid. Fitoterapia (2011) vol.82: p p

513–52

Gofur, A.,Witjoro, A., Ningtiyasa,E.W., Setyowatia, E., Mukharromaha, S.A, Suhartini, Atho’illah, M.F., Lestaria, S.R. 2018. The ameliorative effect of black soybean and purple sweet potato to improve sperm quality through suppressing reactive oxygen species (ROS) in type 2 diabetes mellitus rat (Rattus novergicus). ScienceAsia. 44 : 303-310

Nimse, S & Pal, D. Free Radical, Natural Antioxidant, and their Reaction Mechanism. The Royal Society Of Chemistry (2015). Vol 5. Pp.279-280.

Mukherjee Biswajit, Chowdhury M. Hossain, Laboni Mondal, Paramita Paul, and Milton K. Ghosh. Obesity and Insulin Resistance: An Abridged Molecular Correlation. publisher and licensee Libertas Academica (2013)vol .6: pp.1–11

Valko M, Leibfritz D, Moncol J, Cronin M, Mazur M et al. Free radicals and antioxidants in normal physiological functions and human disease. Int JBiochem Cell Biol (2007). Vol 39 (1): pp. 44-84.

Koulmanda, A. Qipo, H. Auchincloss, Jr & R. N. Smith. Effects of streptozotocin on autoimmune diabetes in NOD mice. Clin Exp Immunol (2003) .vol 134: pp.210–216

Halliwell, B. Reactive Species, and Antioxidants. Redox Biology Is a Fundamental Theme of Aerobic Life. American Society of Plant Biologists (2006). Vol. 141, pp. 312–322

Gofur,A.,Witjoro, A., Ningtiyasa,E.W., Setyowatia, E., Mukharromaha, S.A, Suhartini, Atho’illah, M.F., Lestaria, S.R. 2019. The Evaluation of Dietary Black Soybean and Purple Sweet Potato on Insulin Sensitivity in Streptozotocin - Induced Diabetic Rats. J Pharmacogn .11(4):639-646.





DOI: https://doi.org/10.22146/mot.51544

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