Potency of Sunkist Orange (Citrus sinensisL. Osbeck) against Kidney Histology of White Wistar Rats Induced by Gentamicin

https://doi.org/10.22146/mot.49107

Masdalena Nasution(1), Chrismis Novalinda Ginting(2*), Edy Fachrial(3), I Nyoman Ehrich Lister(4)

(1) Master in Biomedical Sciences Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera
(2) Department of Physiology Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera
(3) Laboratory of Biomolecular Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera
(4) Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera
(*) Corresponding Author

Abstract


About 1.7%-58% of all cases of acute kidney failure are caused by gentamicin nephrotoxicity and consequently increasing urea and creatinine levels in the blood which are indications of damage to function. Oranges contain secondary metabolites such as flavonoids, alkaloids, coumarin, limonoid, keratinoid and essential oil that have pharmacological activities such as antioxidants, anti-inflammatory, anti-cancer, and also nephroprotector. The purpose of this study is to evaluate the nephroprotective activity of Citrus sinensis peel ethanol extract (EEKJS) on rats induced by gentamicin. Rats were induced using gentamicin at a dose of 80 mg/kgBW intraperitoneally on the 7th day after induced at a dose of 50 mg/kgBW, 100 mg/kgBW, and 200 mg/kgBW for 7 days. The results obtained from the ethanol extract of Citrus sinensis peel gave nephroprotective with the lowest serum creatinine and urea levels at a dose of 200 mg/kgBW which was 0.4±0.02 mg/dl and 43.33 ± 2.51 mg/dl and difference significantly (p<0.05) with a positive control group which was only induced by gentamicin and histopathological results showed significant cell damage in the positive control group that was only induced by gentamicin, and in the 50 mg/kgBW dose group, 100 mg/kgBW, 200 mg/kgBW had cell repair after gentamicin induction. Citrus sinensis are highly recommended to be a food supplement for kidney protection.


Keywords


Nephroprotective; Citrus sinensis peel; Gentamicin; Creatinine; Urea

Full Text:

PDF


References

Adil, M., Kandhare, A.D., Dalvi, G., Ghosh, P., Venkata, S., Raygude, K.S. &Bodhankar, S.L. 2016. ‘Ameliorative effect of berberine against gentamicin-induced nephrotoxicity in rats via attenuation of oxidative stress, inflammation, apoptosis and mitrochondrial dysfunction’, Renal Failure. 38. 996-1006.

Bhusan, S.H., Ranjan, S.S. Subhangankar, N., Rakesh, S. & Amrita B. 2012. ‘Nephroprotective activity of ethanolic extract of ElephantophusScaber leaves on albio rats’, International Research Journal of Pharmacy. 3. 246-250.

Donna, L.D., Iacopetta, D., Capello, A.R., Gallucci, G., Martello, E., Fiorillo, M., Dolve, V. &Sindona, G. 2014. ‘Hypocholesterolaemic activity of 3-hydroxy-3-methyl-glutaryl flavonones enriched fraction from bergamot fruit (Citrus bergamia): “in vivo” studies. Journal of Functional Foods. 7. 558-568.

Dosoky, N.S. & Setzer, W.N. 2018. ‘Biological activities and safety of Citrus spp. essential oils’, Int. J. Mol. Sci. 19. 1-25.

Etebu, E. &Nwauzoma, A.B. 2014. ‘A Review on Sweet Orange (Citrus SinensisL Osbeck): Health, Diseases and Management. American Journal of Research Communication. 2. 33-70.

Favela-Hernandez, J.M.J., Gonzalez-Santiago, O., Ramirez-Cabrera, M.A., Esquivel-Ferrino, P.C. & Camacho-Corona, M.D.R. 2016. ‘Chemistry and Pharmacology of Citrus sinensis’, Molecules, 21. 1-24.

Geetha, K.M., Sridhar, C. &Murugan, V. 2010. ‘Antioxidant and healing effect of aqueous alcoholic extract of Rhodomyrtustomentosa(Ait.)Hasskon chronic gastric ulcers in rats’, Journal of Pharmacy Research. 3. 2860-2862.

Gelaw, B.K., Tegegne, G.T., Defersha, A.D. &Legese, A. 2014. ‘Retrospective drug use evaluation of gentamycin use in Ambo Hospital, Oromia Region State, West Showa, Ethiopia’, IJOPP. 7, 16-22.

Lavanya, G., Voravuthikunchai, S.P &Towatana, N.H. 2012. ‘Acetone extract from Rhodomyrtustomentosa: a potent natural antioxidant’, Evidence-Based Complementary and Alternative Medicine. 2012.1-8.

Novoa, J.M.L., Quiros, Y., Vicente, L., Morales, A.I &Hernandez, F.J.L. 2011. ‘New insights into the mechanism of aminoglycoside nephrotoxicity: an integrative point in view’, International Society of Nephrology. 79. 33-45.

Pandey, A. &Tamta, S. 2016. ‘Efficient micropopagation of Citrus sinensis(L.)Osbeck from cotyledonary explants suitable for the development of commercial variety’, African Journal of Biotechnology. 15. 1806-1812.

Pepe, G., Sommela, E., Cianciarulo, D., Ostacolo, C., Manfra, M., Sarno, V.D. et al. 2018. ‘Polyphenolic extract from tarocco (Citrus sinensisL. Osbeck) clone “Lempso” exerts anti-inflammatory and antioxidant effects via NF-kB and Nrf-2 activation in murine macrophages’, Nutrients. 10. 1-12.

Randjelovic, P., Veljkovic, S., Stojiljkovic, N., Sokolovic, D. &Ilic, I. 2017. ‘Gentamicin nephrotoxicity animals: current knowledge and future prespectives’, EXCLI Journal. 16. 388-399.

Tahir, E.E., Shaddad, S.A., Muddathir, A.K. &Agabna N.M.E. 2016. ‘Nephro-protective effect of Acica Senegal against gentamycin-induced renal damage in rats’, World Journal of Pharmaceutical Research. 5. 294-303.

Udupa, V.& Prakash, V. 2019. ‘Gentamicin induced acute renal damage and its evaluation using urinary biomarkers in rats’, Toxicology Reports. 6. 91-99.

Yarijani, Z.M., Najafi, H. &Madani, S.H. 2015. ‘Protective effect of crocin on gentamicin-induced nephrotoxicity in rats’, Irian J Basic Med Sci. 19. 337-343.




DOI: https://doi.org/10.22146/mot.49107

Article Metrics

Abstract views : 2080 | views : 2629

Refbacks

  • There are currently no refbacks.




Copyright (c) 2020 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada