The Anti-Inflammatory Activity of Humic Acid from Borneo Peat Soil in Mice

Rusliandi Rusliandi(1), Diah Wulandari Rousdy(2*), Mukarlina Mukarlina(3)

(1) Departement of Biology, Faculty of Mathematics and Natural Sciences, Tanjungpura University, Pontianak, Kalimantan Barat
(2) Departement of Biology, Faculty of Mathematics and Natural Sciences, Tanjungpura University, Pontianak, Kalimantan Barat
(3) Departement of Biology, Faculty of Mathematics and Natural Sciences, Tanjungpura University, Pontianak, Kalimantan Barat
(*) Corresponding Author


Humic acid is a humus compound found in peat soil. Humic acid can potentially be used as an anti-inflammatory compound. This study aimed to determine the effect of humic acid on the volume of foot mice edema and to find the best dose that can suppress the degree of edema volume. The animal object was Swiss mice weighing 25-30 grams and 3 months old. The study used Randomized Block Design (RBD) with positive control, negative control and humic acid treatment with dose 62.5 mg kg-1BW, 125 mg kg-1BW, and 250 mg kg-1BW. The result of this research showed that edema inhibition by the administration of humic acid dose 62,5; 125; 250 mg kg-1 had inflammatory inhibition percentage 2.67%, 13.34%, and 20.01% respectively in 5-hour observation. The best dose of humic acid to suppress inflammation in the mice's paw is a humic acid dose of 250 mg kg-1 compared with value 23.3% of sodium diclofenac as the positive control.


Humic Acid; Peat; Edema; Carrageenan

Full Text:



Agustian, P.S., & Gusnidar. 2004, ‘Pembentukan asam humat dan fulvat selama pembuatan kompos jerami padi’, Jurnal Solum, 1(1), pp.9–14.

Ammar, N.M., Al-Okbi, S.Y. & Muhamed, D.A., 2005, ‘Study of the antiinflammatory activity of some medical edible plants growing in egypt’, Journal of Islamic Academy of Sciences, 10(4).

Apridamayanti, P., Sanera, F., Robiyanto, R., 2018, Antiinflammatory Activity of Ethanolic Extract from Karas Leaves (Aquilaria malaccencis Lamk.), Pharmaceutical Sciences and Research, 5(3), pp. 152 - 158

Badan penelitian dan pengembangan pertanian, 2011, Peta lahan gambut indonesia skala 1:250.000, Cimanggu Bogor, Kementerian Pertanian.

Besral, 2010, ‘Pengolahan dan analisa data menggunakan SPSS’, Departemen Biostatistika Fakultas Kesehatan Masyarakat’, Depok, Universitas Indonesia.

Chakraborty, A., Devi, R.K.B., Rita S., Sharatchandra, K.H. & Singh, T.I., 2004, ‘Preliminary studies on antiinflammatory and analgesic activities of Spilanthes acmella in experimental animal models’, Indian Journal Pharmacology, 36(3), pp.148-150.

Constance, E., Jansen, V.R. & Pleter, J.N, 2009, ‘Potassium humate inhibits complement activation and the production of inflammatory cytokines in vitro, Inflammation, 32(24), pp. 270-275.

Gau, R.J., Yang, H.L., Chow, S.N., Suen, J.L. & Lu, F.J., 2000, ‘Humic acid suppresses the LPS-induced expression of cell-surface adhesion proteins through the inhibition of NF-kB activation’, Toxicology Applied Pharmacology, 166(1), pp.59-67.

Johan, P.N.W., 2000, ‘An evaluation of the antiinflammatory properties of a brown coal derived potassium humate’, Disertasi, University of Pretoria.

Joone, G.K., Dekker, J. & Jansen van Rensburg C.E., 2003, ‘Investigation of the immunostimulatory properties of oxihumate’, Z Naturforsch, 58(1), pp.263-267.

Joone, G.K. & Jansen van Rensburg, C.E., 2004, ‘An in vitro investigation of the antiinflammatory properties of potassium humate’, Inflammation, 28(3), pp.169-174.

Junek, R., Morrow, R., Schoenherr, J., Schubert, R., Kallmeyer, R., Phull, S. & Klocking, R., 2009, ‘Bimodal effect of humic acids on the LPS-induced TNF-α release from differentiated U937 cells’, Phytomedicine 16, pp.470–476.

Klaus, L., 2003, ‘The role of selectins in inflammation and disease’, TRENDS in molecular medicine, 9(6), pp.263-269.

Kodama, H., & Denso, 2007, ‘Antitumor effect of humus extract on murine transplantable L1210 Leukemia’, Jurnal Veterinary Medicinal Sciences, 69(10), pp.1069-1071.

Kodama, H., Denso & Tsuyoshi, N., 2007, ‘Protection against atypical Aeromonas salmonicida infection in carp (Cyprinus carpio L.) by oral administration of humus extract’, Jurnal Veterinary Medicinal Sciences, 69(4), pp.405-408.

Mark, W.M. & Saunders, M.E., 2006, ‘The immune response’, Lebedeva, T, and Picknett, V. (eds), Elsevier, London.

Tan, K.H., 1995, ‘Dasar-dasar kimia tanah’. Yogyakarta, Gadjah Mada University Press.

Raji, Udoh, U.S., Oluwadara, O.O., Akinsomisoye, O.S., Awobajo, O., & Adhesoga, K., 2002, ‘Anti-inflamatory and analgesic properties of the rhizome extract of Zingiber officinale’, African Journal of Biomedical Research, 5(1), pp.121-124.­

Ricciotti E & Fitzgerald G.A., 2011. ‘Prostaglandins and Inflammation’, Arterioscler Thromb Vasc Biol, 31(5), pp.986–1000. doi:10.1161/ATVBAHA.110.207449

Rousdy, D.W., Rahmawati and Kurniatuhadi R., 2016, ‘Immune responses of wistar rat (Rattus novergicus) on addition of humic acid from borneo peat soil’, Biosaintifika, 8(3), pp.400-405.

Rousdy, D.W. & Wijayanti N., 2016. ‘Peningkatan Imunitas Nonspesifik Ikan Mas, Cyprinus carpio (Linnaeus, 1758) yang Diinfeksi Aeromonas hydrophilla dengan Pemberian Asam Humat Tanah Gambut’, Jurnal Iktiologi Indonesia, 16(3), pp.345-352.

Rousdy, D.W. & Wardoyo, E.R.P., 2018, ‘Histologi limpa dan hematologi mencit yang diinfeksi Escherichia coli setelah pemberian asam humat gambut kalimantan’, Jurnal Bioteknologi dan Biosains Indonesia, 5(2), pp.54-62.

Sahlan, M., Devina, A., Pratami, D.K., Situmorang, H.,Farida, S., Munim,A., Kusumoputro, B., Yohda, M., Faried A., Gozan M., Ledyawati, M., 2019,

Schepetkin, I.A., Khlebnikov, A.I., Ah, S.Y., Woo, S.B., Jeong, C.S., Klubachuk, O.N. & Kwon, B.S., 2003, ‘Characterization and biological activities of humic substances from mumie’, Journal of Agriculture Food Chemistry, 51(1), pp.5245-5254.

Turnbach, M.E., Spraggins, D.S. & Randich, A., 2002, ‘Spinal administration of prostaglandin E2 or prostaglandin F2α primarily produces mechanical hyperalgesia that is mediated by nociceptive specific spinal dorsal horn neuron’, Pain, 97(1), pp.33-45.

Vinegar, R., Truax, J.L. & Selph, J.L., 1976, ‘Quantitive studies of the pathway to acute carrageenan inflamation’, Federation Proceeding.

Winter, C.A., Risley, E.A. & Nuss, G.W., 2002, ‘Carrageenanin induced udem in hind paw of the rat as an assay for antiinflamatory drugs’, Proceding Soc. Exp. Sol. Med. 111, pp.544-547.

Zapolska, D.D. & Naruszwicz, M., 2009, ‘Propionate reduces the cytokine-induced VCAM-1 and ICAM-1 expression by inhibiting nuclear factor-kB (NF-kB) activation’, Journal of Physiology and Pharmacology, 60(2), pp.123-131.


Article Metrics

Abstract views : 2244 | views : 2475


  • There are currently no refbacks.

Copyright (c) 2020 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada