Ethanol Extract of Detam 1 Soybean Seed (Glycine Max L. Merr) for Chronic Kidney Disease Therapy by In Vitro Study

https://doi.org/10.22146/mot.45053

Sijani Prahastuti(1), Meilinah Hidayat(2), Stella Tinia Hasiana(3), Wahyu Widowati(4*), Annisa Amalia(5), Rismawati Laila Qodariah(6), Rizal Rizal(7), Satrio Haryo Benowo Wibowo(8), Hanna Sari Widya Kusuma(9)

(1) Faculty of Medicine, Maranatha Christian University, Jl. Prof. Drg. Surya Sumantri 65, Bandung 40163, West Java, Indonesia
(2) Faculty of Medicine, Maranatha Christian University, Jl. Prof. Drg. Surya Sumantri 65, Bandung 40163, West Java, Indonesia
(3) Faculty of Medicine, Maranatha Christian University, Jl. Prof. Drg. Surya Sumantri 65, Bandung 40163, West Java, Indonesia
(4) Faculty of Medicine, Maranatha Christian University, Jl. Prof. Drg. Surya Sumantri 65, Bandung 40163, West Java, Indonesia
(5) Aretha Medika Utama, Biomolecular Research Center, Jalan Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia
(6) Aretha Medika Utama, Biomolecular Research Center, Jalan Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia
(7) Aretha Medika Utama, Biomolecular Research Center, Jalan Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia
(8) Aretha Medika Utama, Biomolecular Research Center, Jalan Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia
(9) Aretha Medika Utama, Biomolecular Research Center, Jalan Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia
(*) Corresponding Author

Abstract


Chronic Kidney Disease (CKD) has increased incidence and prevalence in developing nations. In this in vitro study, we evaluated the cells proliferative effects, fibronectin (FN), transforming growth factor β (TGF-β1), and Reactive oxygen species (ROS) - level inhibition potential of ethanol extract of detam 1 soybean seed (EEDS) on glucose-induced kidney mesangial cells (SV40 MES 13). The cells proliferation assay used 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)2H-tetrazolium (MTS) assay. FN and TGF-β1 level were measured using ELISA assay kit and ROS level using flow cytometry. Level of   FN, TGF-β1 and ROS, on CKD cells model (5 mM, 10 mM glucose-induced mesangial cell) treated with EEDS 6.25 µg/mL were lower significantly compared to positive control,  EEDS improve cells viability and decrease FN, TGF-β1 and ROS level in glucose-induced kidney mesangial cells as CKD cells model.


Keywords


chronic kidney disease; cytotoxic fibronectin; ROS-DCFDA; soybean; TGF-β1

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References

Chen, C., Cheng, K., Chang, A. Y., Lin, Y., Hseu, Y., Wang, H. 2012. 10-Shogaol, an Antioxidant from Zingiber officinale for Skin Cell Proliferation and Migration Enhancer. Int J Mol Sci 13(2):1762-77.

Dai, M.M., Wu, H., Li, H., Chen, J., Chen, J.Y., Hu, S.L., et al. 2014. Effects And Mechanisms of Geniposide On Rats With Adjuvant Arthritis. Int Immuno Pharmacol. 20(1): 46-53.

Dounousi, E., Papavasiliou, E., Makedou, A., Loannou, K., Katopodis, K.P., Tselepis, A., et al. 2006. Oxidative Stress Is Progressively Enhanced With Advancing Stages of CKD. Am J Kidney Dis 48:752– 760.

Garcı´a-Sa´nchez, O., Lo´pez-Herna´ndez, F.J., Lo´pez-Novoa, J.M. 2010. An Integrative View On The Role Of TGF-B In The Progressive Tubular Deletion Associated With Chronic Kidney Disease. Kidney Int 77: 950-955.

Gemede, H. F., Ratta, N. 2014. Antinutritional Factors In Plant Foods: Potential Health Benefits And Adverse Effects. Int J Nutr Food Sci 3(4): 284-289.

Gilmore, B. L., Liang, Y., Winton, C. E., Patel, K., Karageorge, V., Varano, A. C., et al. 2017. Molecular Analysis Of BRCA1 In Human Breast Cancer Cells Under Oxidative Stress. Sci Rep 7(1): 43435

 

 

 

 

 

Ha, H., Yu, M. R., Kim, K. H. 1998. Melatonin and Taurine Reduce Early Glomerulopathy in Diabetic Rats. Free Radic Biol Med 26(7):944-950.

Han, H., Baik, B. K. 2008. Antioxidant Activity and Phenolic Content of Lentils (Lens culinaris), Chickpeas (Cicer arietinum L.), Peas (Pisum sativum L.) and Soybeans (Glycine max), and Their Quantitative Changes during Processing. Int J Food Sci Technol 43:1971-1978

Han, H., Cao, A., Wang, L., Guo, H., Zang, Y., Li, Z., et

         al. 2017. Decoction Ameliorates Streptozotocin-Induced Rat Diabetic Nephropathy Through Antioxidant And Regulation of The TGF-Β /MAPK /PPAR-Γ Signaling. Cell Physiol Biochem 42(5):1934-44.

Hayata, M., Kakizoe, Y., Uchimura, K., Morinaga, J., Yamazoe, R., Mizumoto, T., et al. 2012. Effect Of A Serine Protease Inhibitor On The Progression Of Chronic Renal Failure. Am J Physiol Renal Physiol 303: F1126–F1135.

Hidayat, M.,  Prahastuti,  S.,  Fauziah,  N.,  Maesaroh,

         M., Balqis, B., Widowati, W. 2016. Modulation of Adipogenesis-Related Gene Expression by Ethanol Extracts of Detam 1 Soybean and Jati Belanda Leaf in 3T3-L1 Cells. Bangladesh J Pharmacol 11: 697-702.

Hidayat, M., Soeng, S., Prahastuti, S., Erawijantari, P. P., Widowati, W. 2015. Inhibitory Potential of Ethanol Extract of Detam 1 Soybean (Glycine max) Seed and Jati Belanda (Guazuma ulmifolia) Leaves on Adipogenesis and Obesity Models in 3T3-L1 Cell Line. JSRR 6(4): 304-12.

Hou, Q., Lei, M., Hu, K., Wang, M. 2015. The Effects of High Glucose Levels on Reactive Oxygen Species-Induced Apoptosis and Involved Signaling in Human Endothelial Cells. Cardiovasc Toxicol 15(2): 140-6.

Jha, C. J., Banal, C., Chow, B. S. M., Cooper, M. E., Dahm, K. J. 2016. Diabetes and Kidney Disease: Role of Oxidative Stress. Antioxid Redox Signal 25(12):657-84.

Juan, M. Y., Chou, C. C. 2010. Enhancement of Antioxidant Activity,Total Phenolic and Flavonoid Content of Black Soybeans by Solid State Fermentation with Bacillus subtilis BCRC14715. Food Microbiol 27(2010):586-591.

Kurtz, A., Oh, S. 2012. Age Relted Changes of The Extracelullar Matrix and Stem Cell Maintenance. Prev Med 54(2012):S50-S56.

Lee, H. S., Song, C. Y. 2009. Oxidized Low-Density Lipoprotein and Oxidative Stress In The Development Of Glomerulosclerosis. Am J Nephrol 29(1):62-70.

Loeffler, I., Wolf, G. 2013. Transforming Growth Factor-β and The Progression of Renal Disease. Nephrol Dial Transplant 29(1):37-45.

Manaharan, T., Palanisamy, U. D., Ming, C. H. 2012. Tropical Plant Extract as Potential Antihyperglycemic Agents. Molecules 17(5):5915-23.

Miller, C.G., Pozzi, A., Zent, R., Schwarzbauer, J.E. 2014. Effects of High Glucose on Integrin Activity and Fibronectin Matrix Assembly By Mesangial Cells. Mol Biol Cell 25(16): 2342-2350.

Nahman, N. S., Leonhart, K. L., Cosio, F. G., Hebert, C. L. 1992. Effects of High Glucose on Cellular Proliferation and Fibronectin Production By Cultured Human Mesangial Cells. Kidney Int 41(2): 396-402.

Okuda, S., Languino, L. R., Ruoslahti, E., Border, W. A. 1990. Elevated Expression of Transforming Growth Factor-Beta and Proteoglycan Production in Experimental Glomerulonephritis. Possible Role In Expansion Of The Mesangial Extracellular Matrix. J Clin Invest 86: 453–462

Pankov, R., Yamada, K. M. 2004. Non-radioactive Quantification of Fibronectin Matrix Assembly. Curr Protoc Cell Biol. Chapter 10: 10-13.

Peng, H., Li, W., Li, H., Deng, Z., Zhang, B. 2017. Extractable and Non-Extractable Bound Phenolic Compositions and Their Antioxidant Properties In Seed Coat And Cotyledon of Black Soybean (Glycine max (L.) merr). J Funct Food 32: 296-312.

Prahastuti, S.,  Hidayat, M., Hasianna,S. T., Widowati, W., Amalia, A., Yusepany,D.T., Rizal, R. Kusuma, H.S.W. 2019a. Antioxidant potential ethanolic extract of Glycine max (l.) Merr. Var. Detam and daidzein. J Phys Conf Ser 1374: 012020

Prahastuti, S.,  Hidayat, M., Hasianna, S. T., Widowati, W., Amalia, A., Qodariah, R.L., Rizal, R.,  Kusuma, H.S.W. Khoiriyah, Z. 2019b. Ethanol Extract of Jati Belanda (Guazuma ulmifolia L.) as Therapy for Chronic Kidney Disease in In Vitro Model. J Reports Pharmaceutic Sci 8(2):229-235

Ramdath, D. D., Padhi, E. M. T., Sarfaraz, S., Renwick, S., Duncan, A. M. 2017. Beyond the Cholesterol-Lowering Effect of Soy Protein: A Review of the Effects of Dietary Soy and Its Constituents on Risk Factors for Cardiovascular Disease. Nutrients 9:324

Ranich, T., Bhathena, S.J., Velasquez, M.T. 2001. Protective Effects of Dietary Phytoestrogens in Chronic Renal Disease. J Ren Nutr. 11(4): 183-193.

Tylicki, L., Rutkowski, B., Hörl, W. H. 2003. Antioxidants: A Possible Role in Kidney Protection. Kidney Blood Press Res 26: 303-14.

Uchiyama-Tanaka, Y., Matsubara, H., Mori, Y., Kosaki, A., Kishimoto, N., Amano, K., et al. 2002. Involvement of HB-EGF and EGF Receptor Transactivation in TGF Mediated Fibronectin Expression in Mesangial Cells. Kidney Int 62: 799–808.

Widowati, W., Darsono, L., Suherman, J., Fauziah, N., Maesaroh, M., Erawijantari PP. 2016. Anti-Inflammatory Effect of Mangosteen (Garcinia mangostana L.) Peel Extract and Its Compounds In LPS-Induced RAW 264.7 Cells. Nat Prod Sci 22(3): 147-153.

Widowati, W., Rani, A.P., Hamzah, R. A., Arumwardana S, Afifah E, Kusuma HSW, et al. 2017. Antioxidant and Antiaging Assays of Hibiscus sabdariffa Extract and Its Compounds. Nat Prod Sci 23(3): 192-200.

Widowati, W., Laksmitawati, D. R., Wargasetia, T. L., Afifah, E., Amalia, A., Arinta, Y., et al. 2018. Mangosteen Peel Extract (Garcinia mangostana L.) as Protection Agent In Glucose-Induced Mesangial Cell as In Vitro Model Diabetic Glomerulosclerosis. Iran J Basic Med Sci 21(9): 972-977.

Yu, T., Jhun, B. S., Yoon, Y. 2011. High-glucose Stimulation Increases Reactive Oxygen Species Production Through The Calcium and Mitogen-Activated Protein Kinase-Mediated Activation of Mitochondrial Fission. Antioxid Redox Sign 14(3):425-37.


 

 


 



DOI: https://doi.org/10.22146/mot.45053

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