Metabolite Fingerprinting Eleutherine palmifolia (L.) Merr. Using UPLC-QTOF-MS/MS

https://doi.org/10.22146/mot.44883

Roihatul Mutiah(1*), Weka Sidha Bhagawan(2), Burhan Ma'arif(3), Jauhar Maknun Septaza Rahmandika(4)

(1) Department of Pharmacy, Faculty of Medical Health Science, Maulana Malik Ibrahim Islamic State University of Malang
(2) Department of Pharmacy, Faculty of Medical Health Science, Maulana Malik Ibrahim Islamic State University of Malang
(3) Department of Pharmacy, Faculty of Medical Health Science, Maulana Malik Ibrahim Islamic State University of Malang
(4) Department of Pharmacy, Faculty of Medical Health Science, Maulana Malik Ibrahim Islamic State University of Malang
(*) Corresponding Author

Abstract


Eleutherine palmifolia (L.) Merr. (E. palmifolia) is an Indonesian native plant that has the potential to be developed into phytopharmaca. The differences in growth locations are thought to cause variation in the content of metabolite compounds which affect differences in pharmacological activity. This study aims to determine the profile of metabolites E. palmifolia bulb from several regions in Indonesia. The samples were collected from six different locations, namely East Java, Central Java, West Java, East Borneo, Central Borneo, and South Borneo. Sample extraction was carried out using Ultrasonic Assisted Extraction (UAE) method with 96% ethanol. The analysis of the content of metabolites was carried out using UPLC-QTOF-MS/MS with a stationary phase column C18 (Okta Decyl Silica), mobile phase mixture of formic acid /water 0.1/99.9 (v/v), and formic acid/acetonitrile 0,1/99,9 (v/v). The results of the analysis were interpreted using software Masslynx and continued with chemometric analysis using the method Principle Component Analysis (PCA). The results showed that there were differences in the content of the metabolite compounds in E. palmifolia bulb originating from six different regions.


Keywords


Metabolite fingerprinting, Eleutherine palmifolia, UPLC-QTOF-MS/MS

Full Text:

PDF


References

Anissa. 2012. Kajian Metabolomik Rimpang Kunyit Menggunakan Kromatografi Cair-Spektroskopi Massa [Skripsi], Departemen Kimia Institut Pertanian Bogor: Bogor.

Aulia, Savira S., Iyan S, Muchtaridi. Penetapan Kadar Simvastatin Menggunakan Kromatorafi Cair Kinerja Tinggi (Kckt): Review, Farmaka, 14(4):1-8.

Ayunda RD. 2014. Aktivitas Antioksidan Ekstrak Etanol Daun Serai (Cymbopogon citratus) dan Potensinya sebagai Pencegah Oksidasi Lipid [Skripsi]. Departemen Biokimia FMIPA, Institut Pertanian Bogor: Bogor.

Brenton AG, Godfrey AR. 2010. Accurate Mass Measurement: Terminology and Treatment of Data, Journal of American Society for Mass Spectrometry, 21:1821-1835.

Distantina S, Fadhila YC, Danarto W, Fahrurrozi M. 2009. Pengaruh Kondisi Proses pada Pengolahan Euchema cottoni terhadap Rendemen dan Sifat Gel Karagenan, Ekuilibrium, 8(1): 35-40.

Firdaus, R., 2006, Telaah Kandungan Kimia Ekstrak Metanol Umbi Bawang Tiwai (Eleutherine americana (Aubl.) Merr.) [Skripsi], Institut Teknologi Bandung: Bandung.

Handayani H, Sriherfyna FH, Yunianta. 2016. Ekstraksi Antioksidan Daun Sirsak Metode Ultrasounic Bath (Kajian Rasio Bahan: Pelarut dan Lama Ekstraksi). Jurnal Pangan dan Agroindustri, 4 (1): 262- 272.

Hara H, Naoki M, Shinsuke Y, Yasuhisa H, Kuo-HL, Kenneth FB, Ruji M,Yasuhiro I. 1997. Elecanacin, a Novel New Naphthoquinone from the Bulb of Eleutherine americana, Journal Chem, Pharm, Bull, Pharmaceutical Society of Japan, 45 (10):1-8.

Heuberger, A.l.; Corey DB, Kaylyn R. Kirkpatrick, Jessica EP. 2013. Application of Nontargeted Metabolite Profiling to Discover Novel Markers of Quality Traits in an Advanced Population of Malting Barley, Plant Biotechnology Journal, 12, pp. 147-160.

Ieyama T, Maria DPT, Jun K. 2011. α-Glucosidase Inhibitors from the Bulb of Eleutherine americana, Food Chem, 128: 308-311.

Rohman, A.  2009.  Kromatografi untuk Analisis Obat (Edisi I). Graha Ilmu: Yogyakarta.

Singh, Chandra K., Ndiaye, Mary A., Ahmad, Nihal, 2014, Resveratrol and Cancer: Challenges for Clinical Translation. BBA - Molecular Basis of Disease.

Skoog DA, Holler FJ, Crouch SR. 2004. Fundamentals of Analytical Chemistry (eighth edition), Thomson Brooks/Cole: Belmont.

Smoliga J.M., Baur J.A., and Hausenblas H.A., 2011, Resveratrol and health – a comprehensive review of human clinical trials, Mol Nutr Food Res, 55: 1129-1141.

Smriga M, Ando T, Akutsu M, Furukawa Y, Miwa K., Morinaga Y., 2007, Oral treatment with L-lysine and L-arginine reduces anxiety and basal cortisol levels in healthy humans. Biomedical Research (Tokyo, Japan), 28(2):85-90. 

Srivastava M, Bashir AA, Shishir KG, and Shailendra KG.    2010.    Development    of     Resistance

 

Against Blackleg Disease in Brassica Oleracea var. Botrytis Through in Silico Methods, Fungal Genetics and Biology, 47:800–808.

Suminski R.R. Robertson R.J., Goss F.L., Arslanian S., Kang J., Da Silva S., Utter A.C., and Metz K.F., 1997, Acute Effect of Amino Acid Ingestion and Resistance Exercise on Plasma Growth Hormone Concentration in Young Men. International Journal of Sports Nutrition, 7(1):48-60.

Syakhala, Abdu R., Puspitaningrum, D, Purwandari, Endina P. 2015, Perbandingan Metode Principal Component Analysis (PCA) dengan Metode Hidden Markov Model (HMM) dalam Pengenalan Identitas Seseorang Melalui Wajah, Jurnal Rekursif, 2(3):68-81

Takahashi T, Nobuo T, Masaaki L, Masaki B, Hoypku N, Hiroyuki T, Toru O. 2004. Isoliquiritigenin, a flavonoid from licorice, reduces prostaglandin E 2  and nitric oxide, causes apoptosis and suppresses aberrant crypt foci development. Cancer Sci, 95(5): 448-453.

Taleuzzaman M, Ali S, Gilani SJ, Imam SS, Hafeez A. 2015. Ultra Performance Liquid Chromatography (UPLC) - A Review, Austin J Anal Pharm Chem, 2(6): 1056.

Taufik I. 2017. Profil Metabolite Kulit Batang Artocarpus champeden Spreng Secara HPTLC Densitometry serta Hubunganna dengan Antimalaria dan Toksisitas In Vitro [Skripsi], Prodi Magister Ilmu Farmasi UNAIR: Surabaya.

Verma N, Shukla S. 2015. Impact of Various Factors Responsible for Fluctuation in Plant Secondary Metabolites, Journal of Applied Research on Medicinal and Aromatic Plants, 2(4) : 49.

Wang N, Zhiyu W, Cheng P, Jieshu Y, Jiangang S, Shouwei H, Jianping C. 2013. Dietary Compound Isoliquiritigenin Inhibits Breast Cancer Neoangiogenesis via VEGF/VEGFR-2 Signaling Pathway, Plos One, 8(7):582-601.

Wolfendera, Jean l, Guillaume M, Aurelien T, and Samuel B. 2015, Current Approaches and Challenges for the Metabolite Profiling of Complex Natural Extracts, Journal of Chromatography A, 1382: 136–164.

 

 

 

 


 

 



DOI: https://doi.org/10.22146/mot.44883

Article Metrics

Abstract views : 332 | views : 294

Refbacks

  • There are currently no refbacks.




Copyright (c) 2019 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada