Determination of Total Xanthone Content in the Preparation of Mangosteen Pericarp Capsules (garcinia mangostana l.) Available on the Market using UV-Visible Spectrophotometry Method

https://doi.org/10.22146/mot.43871

Delladari Mayefis(1*), Yandri Anugerah(2), Roslinda Rasyid(3)

(1) Bachelor of Pharmacy, Mitra Bunda Persada College of Health Sciences, Batam
(2) Bachelor of Pharmacy, Mitra Bunda Persada College of Health Sciences, Batam
(3) Faculty of Pharmacy, Andalas University, Padang
(*) Corresponding Author

Abstract


The skin of Mangosteen fruit (Garcinia mangostana L.) is widely used as traditional medicine, for it contains a lot of xanthone compounds. Today, there are many products from mangosteen pericarp extract being sold on the market, one of which is the preparation of mangosteen pericarp capsules. Determining the total xanthone content in the preparation of mangosteen pericarp capsules (Garcinia mangostana L.) circulating on the market needs to be done to calculate the levels of xanthone that are not included in the preparation label. The results of the analysis with UV-Visible Spectrophotometry obtains the maximum absorption wavelength at 243 nm and the linear regression equation of the calibration curve that is y=0,0568+0,0727x with a value of r=0,9999. Validation of the analysis method showed the results of intraday precision obtained % RSD 0,34%, 0,17%, and 0,16%, interday precision of 0,23%, 0,35%, and 0,16%. The accuracy obtained was mean % recovery 95,55%, limit of detection 0,35 μg/ml and limit of quantitation 1,15 μg/ml. The results of the determination of the total xanthone content in the mangosteen pericarp capsules were 100,8 μg/mg in sample A; 197 μg/mg in sample B; and 50,2 μg/mg in sample C.


Keywords


Mangosteen pericarp capsule; Determination; UV-Visibel Spectrophotometry; Total Xanton



References

Agustina, R., Andayani, R.,and Dachriyanus. (2014). Development and validation of Thin-Layer Chromatographic method for determination of α-mangostin in young pericarp , ripe pericarp and bark extract of Garcinia mangostana L. using TLC-Densitometry. Int. J. Res. Pharm. Sci, 5(4), 294-298.

Ahmad, N.R. (2012). Cara mudah mencegah dan mengobati kanker dengan ramuan tradisional dan alami. Yogyakarta : Aulya Publishing.

Aisha, A.F.A., Abu-Salah, K.M., Ismail, Z., and Abdul Majid, A.M.S. (2013). Determination of total xanthones in Garcinia mangostana fruit rind extracts by ultraviolet (UV) spectrophotometry. J. Med. Plants Res, 7(1), 2935.

Andayani, R., Novita, R., and Verawati. (2015). Pengaruh metode ekstraksi terhadap kadar xanthone total dalam ekstrak kulit buah manggis matang (Garcinia mangostana L.) dengan metode spektrofotometri ultraviolet. Prosiding Seminar Nasional & Workshop *Perkembangan Terkini Sains Farmasi & Klinik 5*, 353-361.

Chaverri, J.P., Rodriguez, N.C., Ibarra, M.O., and Rojas, J.M.P. (2008). Medicinal Properties of Mangosteen (Garcinia mangostana). Food and Chem. Tox, 46, 3227-3239.

Gandjar, I.G. and Rohman, A. (2012). Analisis Obat Secara Spektrofotometri dan Kromatografi. Yogyakarta : Pustaka Pelajar

Harborne, J.B. (2006). Metode Fitokimia : Penuntun Cara Modern Menganalisis Tumbuhan. Edisi Ke-2. Bandung : ITB.

Harmita. (2014). Analisis Fisikokimia : potensiometri & spektroskopi. Jakarta : EGC.

Harmita. (2004). Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitungannya. Majalah Ilmu Kefarmasian, 1(3), 117-135.

Mahabusarakam, W., Kuaha, K., Wilairat, P., and Taylor, W.C. (2006). Prenylated xanthones as potential antiplasmodial substances, Planta Med., 72(10), 912-916.

Muchtaridi,M., Suryani,D., Qosim, W.A., Saptarini, N.M. (2016). Quantitative analysis of α-mangostin in mangosteen (garcinia mangostana l.) Pericarp extract from four district of west java by hplc method. Int J Pharm Pharm Sci, 8(8), 232-236.

Pasaribu, F., Sitorus, P.,and Bahri, S. (2012). Uji Ekstrak Etanol Kulit Buah Manggis (Garcinia mangostana L.) Terhadap Penurunan Kadar Glukosa Darah. Journal of Pharmaceutics and Pharmacolog, 1(1), 1-8.

Pothitirat, W. and Gritsanapan, W. (2008). Quantitative analysis of total mangostins in Garcinia mangostana fruit rind. J Health Res, 22(4), 161-166.

Pradeep kumar, S.V., S. B. Puranik., and Nandini, B. N. (2017). Evaluation of Alpha-Mangostin, Isolated and Purified from the Crude Extract of Garcinia mangostana for the Anti-Diabetic, Anti-Inflammatory and Antioxidant Activity. Ijppr.Human, 8(2), 75-95.

Priya, V., Jainu, M., Mohan, S.K., Saraswathi, P., and Gopan, C.S. (2010). Antimicrobial Activity Of Pericarp Extract Of Garcinia Mangostana Linn. International Journal of Pharma Sciences and Research (IJPSR), 1(8), 278-281.

Putri, A.Y., Yurina, V., and Hidayati, D.Y.N. (2016). α-Mangostin Dari Ekstrak pericarp Manggis (Garcinia mangostana L.) Mampu Menghambat Sekresi Culture Filtrate Protein-10 (CFP-10) pada Mycobacterium tuberculosis H37Rv. Pharmaceutical journal of indonesia, 2(1), 12-17.

Setiawati, A., Riswanto, F.O.D., Yuliani, S.H., and Istyastono, E.P. (2014). Anticancer Activity Of Mangosteen Pericarp Dry Extract Against Mcf-7 Breast Cancer Cell Line Through Estrogen Receptor –Α. Indonesian J. Pharm, 25(3), 119-124.

Suparni, I. and Wulandari, A. (2017). Sehat tanpa obat dengan manggis. Yogyakarta : Rapha Publishing.



DOI: https://doi.org/10.22146/mot.43871

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