Effect of Leaf Syzygium aromaticum on Lymphocytes and Macrophages Mice Balb/c

https://doi.org/10.22146/mot.38474

Syahran Wael(1*), Theopilus Wilhelmus Watuguly(2), Didik Wahyudi(3)

(1) Universitas Pattimura, Ambon
(2) Universitas Pattimura, Ambon
(3) Akademi Analisis Kesehatan Nasional Surakarta
(*) Corresponding Author

Abstract


Syzygium aromaticum as an immunomodulator contains main active compound eugenol which is able to stimulate lymphocyte proliferation and the production of macrophages. Lymphocytes have a very important role to provide protection in the body against infection. This study aims to prove the effects of extract Syzygium aromaticum leaf against increased proliferation of lymphocytes, lymphoblast and macrophages of mice Balb/c of induced Salmonella typhimurium. The method used in this study was experimental with post test only control group. Mice Balb/c were divided into 4 groups as a control group and treatment induced of Salmonella typhimurium. The first treatment group were administrated extracts of 15mg /kgbw, the second treatment 75mg/kgbw, the third treatment of 150mg/kgbw for 12days. ANOVA test showed a significant difference in lymphocyte proliferation but not lymphoblast and macrophages.

Keywords


cloves; lymphocytes; lymphoblast; macrophage

Full Text:

PDF


References

Amanda, L., Santos., Gilberto, O., Chierice., Kenneth, S., Alexander., Alan, R., Ellen, Matthewa. 2009. Characterization of the raw essential oil eugenol extracted from Syzygium aromaticum L. J Therm Anal Calorim 96: 821-825.

Apparecido, N., Daniel., Simone, M., Sartoretto., Gustavo, S., Silvana, M., Caparroz-Assef., Ciomar, A., Bersani-Amado., Roberto, Kenji, N., Cuman. 2009. Anti-inflammatory and antinociceptive activities of eugenol essential oil in experimental animal models. Brazilian journal of pharmacognosy 19 (1B) : 212-217.

Banerjee, S., Panda, C,K., Das, S. 2006. Clove (Syzygium aromaticum L.) potential chemopreventive agent for lung cancer. Carcinogenesis 27: 1645-1654.

Bachiega, T.F., Orsatti, C.L., Pagliarona, A.C., Missima, F., Sousa, J.P.B., Bastos, J.K. 2009. Th1/Th2 cytokine production by clove-treated mice. Natural Product Research. 23 (16): 1552-1558.

Bhuiyan, Md, N.I., Bagum, J., Nandi, N.C. and Akter, F. 2010. Constituents of the essential oil from leaves and buds of clove (Syzigium aromaticum). African Journal of Plant Science. 4 (11): 451-454.

Dehghani, F., Heshmatpour, A., Panjehshahin, M.R. and Khozani, T.T. 2012. Toxic effects of water/ alcoholic extract of Syzygium aromaticum on sperm quality, sex hormones and reproductive tissues in male mouse. IUFS J Biol 71 (2): 95 – 102.

Dibazar S P, Fateh S, Daneshmandi S. 2014. Clove (Syzigium aromaticum) ingredients affect lymphocyte subtypes expansion and cytokine profile responses: an in vitro evaluation. Food and drug analysis. 22:448-454.

Dibazar S P, Fateh S, Daneshmandi S. 2015. Immunomodulatiry effects of clove (Syzygium aromaticum) constituents on macrophages: in vitro evaluations of aqueous and ethanolic components. Journal of immunotoxicology. 12 (2) 124-131.

Farizal, J. 2012. Effect of ethanol extract bidara upas tuber (merremia mammosa) to lymphocytes proliferation And ROI macrophage production. Tesis Magister Ilmu Biomedik UNDIP.

Meyer, S, L, F., Lakshman, D, K., Zasada, I, A., Vinyard, B, T., Chitwod, D, J. 2008. Dose response effects of clove oil from Syzygium aromaticum on the root-knot nematode Meloidogye incognita. Pest Manag Sci 64: 223-229.

Nurhidayati, L. dan Sulistiowati. 2013. Penetapan kadar eugenol dalam minyak atsiri dari tiga varietas bunga cengkeh (Syzygium aromaticum(L) Merr. & L.M. Perry) secara kromatografi gas. Seminar Nasional dalam Rangka Lustrum X Fakultas Farmasi Univesitas Pancasila

Prashar A, Locke I.C, Evans C.S. 2006. Cytotoxicity of clove (Syzygium aromaticum) oil and its major components to human skin cell. Cell Prolif 39: 241-248.

Rachel, N.T., Purnawati, R.D., Susilaningsih, N. 2017. Pengaruh pemberian ekstrak daun sirih merah dosis bertingkat terhadap produksi peroksida makrofag: studi pada mencit Balb/C yang diinfeksi Salmonella typhimurium. Jurnal kedokteran Diponegro 6(2): 912-920.

Razafimamonjison G, Jahiel M, Duclos T, Ramanoelina P, Fawbush F, Danthu P. 2014. Bud, leaf and stem essential oil composition of Syzigium aromaticum from Madagascar, Indonesia and Zanzibar. International Journal of Basic and Applied Sciences. 3 (3) : 224-233.

Tursinawati, Y., Dharmana, E. 2015. Efektivitas pemberian kombinasi produk herbal antibiotik terhadap infeksi Salmonella typhimurium pada mencitBalb/c. University Research Coloquium (2): 231-237.

Ulfa, M., Cahyani, V.S.N., Kinasih, I. 2017. Pengaruh pemberian seduhan the daun sirsak (Annona muricata L.) terhadap aktivitas fagositosis sel makrofag dan proliferasi sel limfosit mencit galur Balb/c yang diinduksi vaksin hepatitis B. Momentum. 13 (2): 63-71




DOI: https://doi.org/10.22146/mot.38474

Article Metrics

Abstract views : 3978 | views : 31035

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2018 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada