Optimization of HIB Value Combination of Tween 60 and Span 80 on Cream Formulation of Ethanol Extract of Green Tea Leaves (Camellia Sinensis L.)

https://doi.org/10.22146/mot.38402

Fransiska Lisa Anindya Putri(1*), Akhmad Kharis Nugroho(2), Erna Prawita Setyowati(3)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Green tea (Camellia sinensis L.) is known to have ability to protect skin against free radicals. This is supported by polyphenol compound catechin. This research aims to determine the optimum Hydrophilic-Lipophilic Balance (HLB) value of Tween 60 and Span 80 compositions on the optimum cream formula of ethanol extract of green tea leaves. Tea leaves are extracted by macerating using 70% ethanol. Catechin in extract is known from Thin Layer Chromatography (TLC) test with silica gel 60 F254 as stationary phase and ethyl acetate:aquadest:formic acid (18:1:1 v/v) as mobile phase. Antioxidant activity is determined by 2,2-Diphenyl-1-picryhydrazyl (DPPH) method and value of Inhibition Concentration 50% (IC50) is then calculated. Formula optimization using Design Expert® version 7.1.5 (DX 7) software, Simplex Lattice Design (SLD) method with two components Tween 60 and Span 80. Cream is characterized according to physical properties organoleptic, homogeneity, viscosity, pH, spreadability, adhesiveness, and cream type. The optimum formula obtained is then tested for physical stability for 4 weeks at room temperature (28±2°C) and data are statistically analyzed using one-way ANOVA. The extract contains catechin proved with Retention factor (Rf) value 0.8 and has antioxidant activity with IC50 value 56.35 ppm. 6.4% Tween 60 and 3.6% Span 80 result an optimum HLB value 11.1. It has viscosity 2897.50±35.94 mPa.s, spreadability 18.44±0.06 cm2, adhesiveness 0.85±0.05 seconds, and pH 4.530±0.002. Statistical test shows that the cream is significantly altered at pH, but does not significantly change in viscosity, spreadability, and adhesiveness after being stored for 4 weeks.


Keywords


optimasi, HLB, Tween 60, Span 80

Full Text:

PDF


References

Allen, L. V., Popovich, N. G., Ansel, H. C., 2004, Ansel’s Pharmaceutical Dosage Forms and Drug Delivery Systems, Ninth Edition, 398, Lippincott Williams & Wilkins, Philadelphia.

Amarowicz, R., Maryniak, A., & Shahidi, F., 2005, TLC Separation of Methylated (-)-Epigallocatechin-3-Gallate, Czech Journal of Food Science, 23 (1), 36-39 dalam Rustanti, E., Jannah, A., & Fasya, A. G., 2013, Uji Aktvitas Antibakteri Senyawa Katekin dari Daun Teh (Camellia sinensis L. var assamica) terhadap Bakteri Micrococcus luteus, Alchemy, 2 (2), 138-149.

Bakker, P., Doorne, H. V., Gooskens, V., & Wieringa, N., 1991, Dermatological Preparations for The Tropics : A Formulary of Dermatological Preparations and Backgrounds of Choices, Production and Dispensing, 147-148, Scienceshop for Medicine, University of Groningen, Netherlands.

Chacko, S. M., Thambi, P. T., Kuttan, R., & Nishigaki, I., 2010, Beneficial Effects of Green Tea: A Literature Review, BioMed Centrals, 5 (3), 1-9.

Chen, Y., Wang, M., Rosen, R. T., & Ho, C. T., 1999, 2,2-Diphenyl-1-picrylhydrazyl Radical-Scavenging Active Components from Polygonum multiflorum Thunb., Journal Agriculture Food Chemical, 47, 2226-2228.

Departemen Kesehatan RI, 1979, Farmakope Indonesia, Edisi III, 8, 508-509, 633, Departemen Kesehatan RI, Jakarta.

Departemen Kesehatan RI, 2008, Farmakope Herbal Indonesia, Edisi I, 171, 174-175, Departemen Kesehatan RI, Jakarta.

Mahato, R. I., 2007, Pharmaceutical Dosage Forms and Drug Delivery, 108-111, CRC Press, USA.

Robinson, T., 1995, Kandungan Senyawa Organik Tumbuhan Tinggi, Penerbit ITB, Bandung dalam Rustanti, E., Jannah, A., & Fasya, A. G., 2013, Uji Aktivitas Antibakteri Senyawa Katekin dari Daun Teh (Camellia sinensis L. var assamica) terhadap Bakteri Micrococcus luteus, Alchemy, 2 (2), 138-149.

Rowe, R. C., Sheskey, P. J., & Quinn, M. E., 2009, Handbook of Pharmaceutical Excipients, Sixth Edition, 283, 155, 441-445, 474, 483,

549-552, 675-678, Pharmaceutical Press and American Pharmacists Association 2009, Washington D.C.

Singh, I., Goyal, A., Kumar, S., Nagpal, M., & Arora, S., 2011, Potential of Novel Drug Delivery Systems for Herbal Drugs, Indian Journal of Pharmaceutical Education and Research, 45 (3), 225-235.

Soraya, Noni, 2007, Sehat dan Cantik Berkat Teh Hijau, 61-63, Penebar Plus, Jakarta.


 



DOI: https://doi.org/10.22146/mot.38402

Article Metrics

Abstract views : 186 | views : 252

Refbacks

  • There are currently no refbacks.




Copyright (c) 2018 Majalah Obat Tradisional (Traditional Medicine Journal)

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada