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Physical Characteristic and Antibacterial Activity of Silver Nanoparticles from Green Synthesis Using Ethanol Extracts of Phaleria macrocarpa (Scheff.) Boerl Leaves
https://doi.org/10.22146/mot.37956 Tri Puji Lestari(1*), Fitia Abbas Tahlib(2), Johan Sukweenadhi(3), Kartini kartini(4), Christina Avanti(5)
(1) 1) Faculty of Pharmacy, Universitas Surabaya (UBAYA), Surabaya 3) Faculty of Pharmacy, Institut Ilmu Kesehatan Bhaktiwiyata, Kediri
(2) 1) Faculty of Pharmacy, Universitas Surabaya (UBAYA), Surabaya
(3) 2) Faculty of Biotechnology, University of Surabaya (UBAYA), Surabaya
(4) 1) Faculty of Pharmacy, Universitas Surabaya (UBAYA), Surabaya
(5) 1) Faculty of Pharmacy, Universitas Surabaya (UBAYA), Surabaya
(*) Corresponding Author
Abstract
The Green Synthesis method is widely developed due to its environmentally friendly, cost-effective, and easy application for nanoparticle-scale synthesis. Among all metal nanoparticles, silver nanoparticles are the most utilized products in the field of nanotechnology. Biomolecules contained in plant extracts can reduce the size of silver particles to nano size. This study aims to determine the antibacterial properties and activity of silver nanoparticles synthesized with the ethanol extract of mahkota dewa leaves. The formation of silver nanoparticles is monitored with UV-Vis absorption and its change in color. Parameters evaluated are shape, size, particle size distribution, composition, metal residue, and a functional group of nanoparticles, using Scanning Electron Microscopy (SEM), Particle Size Analyzer (PSA), X-Ray Diffraction (XRD), and Fourier Transform InfraRed (FTIR) instrument. The research on UV-Vis color and absorption show black silver color with the wavelength of 450-465nm. The characterization result shows spherical-shaped silver nanoparticle. Furthermore, PDI best value on concentration of 0.125% is 0,221±0,0482 with average particle size of 130,300±12,6858 nm. The diffraction pattern of silver nanoparticle with XRD test indicates that the nanoparticles contain the silver component. Antibacterial activity test shows that silver nanoparticles have a greater inhibition zone than AgNO3, and 0.125% ethanol extract of mahkota dewa leaves against Escherichia coli and Staphylococcus aureus. From the results of the study, it can be concluded that the ethanol extract from mahkota dewa leaves can be used as a bioreductor agent to produce silver nanoparticles which have greater antimicrobial activity compared to Ag and ethanol extract from mahkota dewa leaves.
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Andrean, D., Prasetyo, S., Kristijarti, A. P., & Hudaya, T. (2014). The Extraction and Activity Test of Bioactive Compounds in Phaleria macrocarpa as Antioxidants. Procedia Chemistry, 9, 94–101.
Charpe, T. W., & Rathod, V. K. (2014). Effect of ethanol concentration in ultrasound assisted extraction of glycyrrhizic acid from Licorice root. Iranian Journal of Chemical Engineering, 11(4), 21–30.
Durán, N., Marcato, P. D., De Souza, G. I. H., Alves, O. L., & Esposito, E. (2007). Antibacterial effect of silver nanoparticles produced by fungal process on textile fabrics and their effluent treatment. Journal of Biomedical Nanotechnology, 3(2), 203–208.
Easmin, M. S., Sarker, I., Ferdosh, S., Shamsudin, H., Yunus, B., Uddin, S., Abdul, H. P.
S. (2014). Bioactive compounds and advanced processing technology : Phaleria macrocarpa ( sheff .) Boerl , a review. J Chem Technol Biotechnol, 90(6), 981-991
Hajimirzababa, H., Rahimi, M. K., & Alibakhshi, S. (2012). Durable Anti-bacterial Nylon Carpet Using Colloidal Nano Silver. Fibres & Textiles in Eastern Europe, 4(93), 96– 101.
Handayani, W. universitas indonesia. (2011). Pemanfaatan Tumbuhan Tropis Untuk Biosintesis Nanopartikel Perak dan Aplikasinya sebagai Indikator Kolorimetri Keberadaan Logam Berat. Tesis,Universitas Indonesia.
Junaidi, A. B., & Wahyudi, A. (2015). Kajian Sintesis
Nanopartikel Perak Pada Komposit Kitosan Dan Polietilena Glikol : Efek Jenis Agen Pereduksi Organik Study on the Synthesis of Silver Nanoparticles Onto Chitosan and Polyethylene Glycol Composites : Effect of Organic Reducing Agent, 3–4.
Kim, Y. J., Wang, C., Mathialagan, R., Farh, M. E., Yang, D. C. (2015). Biogenic silver and gold nanoparticles synthesized using red ginseng root extract , and their applications. Artificial Cells, Nanomedicine, and Biotechnology, 44(3), 811-816.
Matsjeh, S., Pranowo, H. D., & Anwar, C. (2015). Two Isophalerin Compounds From Ethyl Acetate Of Leave And Fruit Of Mahkota Dewa ( Phaleria macrocarpa (Scheff.) Boerl .) And Its Antibacterial Activity, 15(2), 179–186.
Mihardja, L., Soetrisno, U. and Soegondo, S. (2014) ‘Prevalence and clinical profile of diabetes mellitus in productive aged urban Indonesians’, Journal of Diabetes Investigation, 5(5), 507–512.
Nabikhan, A., Kandasamy, K., Raj, A., & Alikunhi, N. M. (2010). Synthesis of antimicrobial silver nanoparticles by callus and leaf extracts from saltmarsh plant, Sesuvium portulacastrum L. Colloids and Surfaces B: Biointerfaces, 79(2), 488–493.
Rojas, R., Bustamante, B., Bauer, J., Fernández, I., Albán, J., & Lock, O. (2003). Antimicrobial activity of selected Peruvian medicinal plants. Ethnopharmacology, 88, 199–204.
Shodikin, M. A. (2010). Antimicrobial Activity of Mahkota Dewa [Phaleria macrocarpa (Scheff). Boerl.] Leaf Extract Againts Pseudomonas aeruginosa by Agar Dilution and Scanning Electron Microscopy. Folia Medica Indonesiana, 46(3), 172–178.
Singh, S., Pai, D. R., & Yuhhui, C. (2013). Diabetic Foot Ulcer – Diagnosis and Management, Clinical Research on Foot & Ankle Research, 1(3), 1–9.
Sulistiawaty, L. (2015). Sintesis Nanopartikel Perak Terstabilkan Gelatin Dan Tween 20 Untuk Deteksi Ion Logam Hg 2+ .Tesis, Institut Pertanian Bogor.
Swanson, H. E., Gilfrich, N. T., & Ugrinic, G. M. (1955). Standard X-Ray Diffraction Powder Patterns. U.S. Government Printing Office, Circular of the National Bureau of Standards 539, 26
Udapudi. B, N., & Tabassum.S, Sharma.R, B. (2012). Synthesis And Characterization Of Silver Nanoparticles. International Journal of Pharmacy and Biological Sciences, 2(4), 781–791.
DOI: https://doi.org/10.22146/mot.37956
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