Activity of 70% Ethanol Extract of Chrysophyllum cainito in Increasing Vertebrae Trabecular Bone Density in Female Mice

Nur Imamah Utaminingtyas(1), Burhan Ma'arif(2*), Dewi Sinta Megawati(3), Ria Ramadhani Dwi Atmaja(4)

(1) Undergraduate Student of Department of Pharmacy, Faculty of Medical and Health Science, Maulana Malik Ibrahim State Islamic University, Malang
(2) Maulana Malik Ibrahim State Islamic University
(3) Department of Pharmacy, Faculty of Medical and Health Science, Maulana Malik Ibrahim State Islamic University, Malang
(4) Department of Pharmacy, Faculty of Medical and Health Science, Maulana Malik Ibrahim State Islamic University, Malang
(*) Corresponding Author


Phytoestrogens are a class of compounds that have estrogen-like functions or structure. This research was conducted to find out the phytoestrogens activity in Chrysophyllum cainito leaves, in increasing the vertebrae trabecular bone density of female mice, using dexamethasone induction as osteoporosis model.  The 70% ethanol extract of C. cainito leaves was given to female mice with dose of 100; 200; and 400 mg/kgBW mice/day after being induced orally with dexamethasone with 0.145 mg/kgBW mice/day. The positive control used was 1.3 mg/kgBW mice/day alendronate. This research had been done in four weeks. The increasing of trabecular vertebrae bone density of female mice was measured with Histomorphometry staining methods. The result showed the 70% ethanol extract of C. cainito leaves have activity in increasing trabecular vertebrae bone density for female mice with optimum dose of 400 mg/kgBW mice/day. This activity occurs because phytoestrogens content in 70% ethanol extract of C. cainito leaves, that may replace the function of estrogen in its bond with ER.


Phytoestrogen; Crysophyllum cainito; Osteoporosis

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Agrawal V and Gupta. 2013. Recent Update On Osteoporosis. Int J Med Sci Public Health. II (2): 164-168.

Ahmed SF and Elmantaser M. 2009. Secondary Osteoporosis. Endocr Dev, 2009; 16 (90): 170.

Brunton LL. 2005. The Pharmacological Basis of Therapeutics. Goodman & Gilman’s. McGraw-Hill, San Diego,.

Constantine, G. D. and Pickar, 2003. J. H. Estrogens in postmenopausal woman: recent insights. Elsevier : Pharmacology. 3 : 626-634.

Dipiro, J. T., Robert, L. T., Gary, C. Y., Barbara G. W. L., Michael, L. 2008. Pharmacotherapy (A pathophysiologic Approach), 7th Edition. United Stated : McGraw-Hill Companies, Inc.

Edwards, B. J., Brooks, E. R., Langman, C. B. 2004. Osteoporosis Screening of Postmenopausal Woman in the Primary Care Setting: A Case-Based Approach. Gender Medicine, 1 (2).

Kawiyana, IKS. 2009. Osteoporosis Patogenesis Diagnosis dan Penanganan Terkini. J. Peny Dalam, 10 (2): 166.

Kementrian Kesehatan RI. 2015. Indofatin Data dan Kondisi Penyakit Osteoporosis di Indonesia. Pusat data dan Informasi Kemenkes RI, Jakarta.

Kini U and Nandeesh BN. 2012. Physiology of Bone Formation, Remodeling, and Metabolism Springer : Radionuclide and Hybrid Bone Imaging.

Koffi N, Amoikon KE, Tiebre MS, Kadja B, and Zirihi GN. 2009. Effect of Aqueous Extract of Chrysophyllum cainito Leaves on The Glycaemia of Diabetic Rabbits. African Journal Pharmacy Pharmacology, 3 (10): 501-506.

Laswati, H., Agil, M., Widyowati, R. 2015. Effect of Spilanthes acmela and Exercise on Osteoblast Cells Femur in Mice Dexamethasone Induced. Media Litbangkes 25 (1): 43-50.

Lee WL, Tsui K, Seow KM, Cheng MH, Su WH, Chen CP, Wang PH. 2013. Hormone therapy for postmenopausal woman and unanswered issue. Elsevier : Gynecology and Minimally Invasive Therapy, 2: 13-17.

Luo XD, Basile, MJ, and Kennely, EJ. 2002. Polyphenolic Antioxidants from Chrysophyllum cainito L. (Star Apple). Journal of Agricultural and Food Chemistry, 50 (6): 1379-1382.

Meeta. 2013. Postmenopause Osteoporosis basic and Clinical Consepts. Jaypee Brothers Medical Publishers, New Delhi, 20-22

Noor Z. 2014. Buku Ajar: Osteoporosis Patofisiologi dan Peran Atom Mineral dalam Manajemen Terapi. Salemba Medika, Jakarta.

Rachman, I. A., Soewondo, P., Setiati, S., Kusumawijaya, K., Baziad, A., Witjaksono, J., Sukarya, W. S., Silvia. 2004. Terapi Sulih Hormon pada Wanita Perimenopause. HTA Indonesia.

Urasopon N, Hamada Y, Cherdshewasart W and Malaivijitnond S. 2008. “Preventive effects of Pueraria mirifica on bone loss in ovariectomized rats,” Maturitas, 59 (2): 137–148.

Villiers TJ. 2009. Bone health and osteoporosis in postmenopausal woman. Elsevier : Best Practice & Research Clinical Obstetrics and Gynaecology, 23: 73-85.

Wibowo A. 2009. Cerdas Memilih Obat dan Mengenali Penyakit. PT. Lingkar Pena Kreativa, Jakarta Selatan.

Yang, T-S., Wang, S-Y., Yang, Y-C., Su, C-H., Lee, F-K., Chen, S-C., Tseng, C-Y., Jou, H-J., Huang, J-P., Huang, K-E. 2012. Effects of standardized phytoestrogen on Taiwanese menopausal woman. Elsevier : Taiwanese Journal of Obstetrics & Gynecology, 51: 229-235.


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