Anti-Tuberculosis Activity of Extract Ethyl Acetate Kenikir Leaves (Cosmos caudatus H.B.K) and Sendok Leaves (Plantago Major L.) By In Vitro Test

https://doi.org/10.22146/mot.35112

Tatang Irianti(1*), Sylvia Utami Tanjung Pratiwi(2), Kuswandi Kuswandi(3), Nanan Tresnaasih(4), Dharmastuti Cahya(5), Fatmarahmi Fatmarahmi(6), Yulia Paramitha(7)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(4) Faculty of Pharmacy, Universitas Gadjah Mada
(5) Faculty of Pharmacy, Universitas Gadjah Mada
(6) Faculty of Pharmacy, Universitas Gadjah Mada
(7) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The increasing therapy problem including multidrug-resistant tuberculosis (TB) has made it important to discover a new anti-TB drug candidate. The aim of this study was to acknowledge the activity of ethyl acetate extracts of kenikir (Cosmos caudatus H.B.K) and sendok (Plantago major L.) leaves against Mycobacterium tuberculosis (M. tuberculosis) H37Rv. This research used Middlebrook (MB) 7H9 media and observed the growth of M. tuberculosis using Lowenstein Jensen (LJ) media. The concentration of extracts were 0.25 mg/ml, 0.50 mg/ml, and 1.00 mg/ml. The result of this study showed that ethyl acetate extracts exhibited anti-TB activity in 1000 µg/ml of both extracts. The active compound group was detected by thin layer chromatography (TLC) and the separation of compounds was shown by retardation factor (Rf) and the color of the spots. Based on TLC chromatograms, it is known that there are types of compounds, such as ortho-dihydroxy compounds, phenolic compounds, and compound leads to terpenoids for both extracts.

Keywords


tuberculosis; ethyl acetate extract; kenikir (Cosmos caudatus H.B.K); sendok (Plantago major L.), leaves; M. tuberculosis; tuberculosis; ethyl acetate extract; kenikir; Cosmos caudatus H.B.K; sendok; Plantago major L.; leaves; M. tuberculosis

Full Text:

PDF


References

Agusta, A., (2000), Minyak Atsiri Tumbuhan Tropika Indonesia, Penerbit: ITB-Ganesha, Bandung, hal. 101

Akbar, Hendra Rizki. 2010. Isolasi dan Identifikasi Golongan Flavonoid Daun Dandang Gendis (Clinacanthus Nutans) Berpotensi Sebagai Antioksidan (Skripsi). Bogor: IPB.

Anonim, 1995, Farmakope Indonesia, Edisi IV, Kementrian Kesehatan RI Jakarta.

Anonim, 2002, Pedoman Nasional Penanggulangan Tuberkulosis Edisi 9, Departemen Kesehatan RI, Jakarta.

Anonim, 2007, Pedoman Nasional Penanggulangan Tuberculosis, Edisi II, Kementrian Kesehatan RI, Jakarta.

Anonim, 2010, Cosmos caudatus Kunth, Malaysian Herbal Monograph, http://www.globinmed.com/index.php?option=com_content&view=article &id=104892:cosmos-caudatus-kunth&catid=209&ltemid=143, 2 September 2016.

Anonim, 2012a, Petunjuk Teknis Pemeriksaan Biakan, Identifikasi, dan Uji Kepekaan Mycobacterium tuberculosis pada Media Padat, Kementrian Kesehatan RI, Jakarta.

Anonim, 2012b, Taxonomy-Mycobacterium tuberculosis complex, www.uniprot.org/taxonomy77643, 19 November 2016.

Brennan, P.J., 2003, Structure, function and biogenesis of the cell wall of Mycobacterium tuberculosis, Tuberculos, 83 : 91-97.

Bunamela, J.G., Martinez Moralis, Stashenko E., and Ribon W., (2014), Antitubercular activity of eleven aromatic and medicinal plants occvuring in Africa, Biomedica, Vol. 29: 51-60

Cantrell, C.L., Franzblau, S.G., Fischer N.H., 2001, Antimycobacterial Plant Terpenoids, Planta Med 67: 685-694..

Coban AY, Cihan CC, & Bilgin K., 2006, Blood agar for susceptibility testing of Mycobacterium tuberculosis against first-line drugs, Int J Tuberc Lung Dis, 10: 450–453.

Dias, H. M., Falzon, D, Fitzpatrick, C., Floyd, K., Glaziou, P., & Hiatt, T., 2012, Global Tuberculosis Report 2012, World Health Organization, Geneva.

Fansworth, N.R., & Fabricant, D.S., 2001,The value of plants used in traditional medicine for drug discovery, Environ Health Perspect, 109: 69-75.

Gritter, R.J., J.M. Robbit, & A.E. Schwarting, 1991, Pengantar Kromatografi, diterjemahkan oleh Kosasih Padmawinata, Edisi 2, 34-81, ITB, Bandung.

Harborne JB., 1987, Metode Fitokimia: Penuntun Cara Modern Menganalisa Tumbuhan, Padmawinata K penerjemah, Penerbit ITB, Bandung. Terjemahan dari: Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis.

Hiswani, 2009, Tuberkulosis Merupakan Penyakit Infeksi Yang Masih Menjadi Masalah Kesehatan Masyarakat, http://library.usu.ac.id/download/fkm-hiswani-6.pdf2009, 7 Januari 2017.

Irianti, T., Puspitasari, A., Machwiyyah, L., dan Rabbani, H.R., (2015a), The Activity of Radical Scavenging 2-2’diphenil-1-Picrylhydrazyl (DPPH) by Ethanolic Extract of Mengkudu leaves, brotowali stem, its water fraction and its hydrolyzed fraction, Traditional Medicine Journal Vol. 20 No. 3, Yogyakarta, Indonesia.

Irianti, T., Puspitasari, A., Suryani, E., Rabbani, HR., Kuswandi and Kusumaningtyas RA. (2015b), Scavenging Activity Of Radical 2-2’diphenil-1-Picrylhydrazyl (DPPH) by Acid Fraction and Aqueous Hydrolyzed Fraction of Tinospora Crispa L. Ethanolic Extract, ICEOH-Johor-Malaysia, Conference.

Jork, H., Werner, F., Walter, F., dan Hans, W., 1990, Thin-Layer Chromatography, Reagen and Detection Methods, VCH Publisher, USA.

Kennedy AD, (2011), Biochemical profil of mycobacterium tuberculosis gron under hypoxic conditions (snow globe model, SG7), (online) www.metabolon.com.

Merfort, I., 2002, Review of the analytical techniques for sesquiterpenes and sesquiterpene lactones, Journal of Chromatography A, 967: 115-130.

Middlebrook, G.M.D dan Cohn, M.L., 1957, Bacteriology of Tuberculosis: Laboratory Methods, American Journal Public Health, 48(7).

Munawaroh, A.L., Dwi Y.N.H., dan Yulian W.U., 2015, Studi komparasi media kultur Coco Blood Malachite Green (CBM) dengan Lowenstein Jensen (LJ) untuk diagnosis cepat, spesifik, dan sensitif pada sputum pasien suspek tuberkulosis, Majalah Kesehatan FKUB, Vol. 2 (2).

Purba, R.D 2001. Analisis Komposisi Alkaloid Daun Handeuleum (Graptophyllum pictum (Linn), Griff) yang Dibudidayakan dengan Taraf Nitrogen yang Berbeda (Skripsi). Bogor: Institut Pertanian Bogor.

Rios dan Recio, 2005, Medicinal plants and antimicrobial activity, Journal of Ethnopharmacology, 100: 80–84.

Rowe, R.C., Shekey, P.J. dan Quinn, M.E., 2009, Handbook of Pharmaceutical Excipients Sixth Edition, Pharmaceutical Press and American Pharmacist Association, USA.

Samuelsen, A.B., 2000, A Review: The traditional uses, chemical constituents and biological activities of Plantago major L., J. Ethnopharmacol., 71, 1-21

Saric, D., Bojic, M., Haas, V.S, dan Males, Z., 2013, Determination of Flavonoids, Phenolic Acids, and Xanthines in Mate Tes (Illex paraguariensis St.-Hill), Journal of Analytical Methods in Chemistry, Vol. 2013.

Sutrisno, (2016), Eksplorasi Potensi Senyawa Organik Bahan Alam Dengan Pendekatan Berbasis Struktur dan Implikasinya Pada Pembelajaran Kimia Organik di perguruan Tinggi, Prosisding Seminar Nasional Kimia, ISBN: 978-602-0951-12-6, Universitas Negeri Surabaya, hal 11.

Taofik, et. al. 2010. Isolasi dan Identifikasi Senyawa Aktif Ekstrak Air Daun Paitan (Thitonia Diversifolia) Sebagai Bahan Insektisida Botani Untuk Pengendalian Hama Tungau Eriophydae. Alchemy Vol. 2(1): 104- 157.

Tirtana, B.T., 2011, Faktor-Faktor yang Mempengaruhi Keberhasilan Pengobatan pada Pasien Tuberkulosis Paru dengan Resistensi Obat Tuberkulosis di Wilayah Jawa Tengah, Skripsi, Fakultas Kedokteran Universitas Diponegoro, Semarang.

Wagner H., Bladt S., dan Zgainski, E. M., 1984, Plant Drug Analysis, A Thin Layer Chromatography Atlas, Springer, Berlin.

Wahyono, 2008, Tanaman Sebagai Sumber Obat-Obatan, Sidang Pengukuhan Guru Besar UGM, Yogyakarta.

World Health Organization (WHO), 2016, Tuberculosis country profiles, http://www.who.int/tb/country/data/profiles/en 18 Oktober 2017.

World Health Organization (WHO), 2003, Adherence to long-term therapies: evidence for action, World Health Organization, Geneva.

World Health Organization (WHO), 2016, Global Tuberculosis Report 2016, World Health Organization, Geneva.



DOI: https://doi.org/10.22146/mot.35112

Article Metrics

Abstract views : 234 | views : 281

Refbacks

  • There are currently no refbacks.




Copyright (c) 2018 Majalah Obat Tradisional (Traditional Medicine Journal)

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada