Determination of γ-oryzanol, Phenolic total content, and (2,2-difenil-1-picrylhydrazyl) Radical Scavenging Activity in Different Varieties of Rice (Oryza sativa) in Yogyakarta, Indonesia

https://doi.org/10.22146/mot.33398

Erna Prawita Setyowati(1*), Andayana Puspitasari Gani(2)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


In Indonesia, beras (Oryza sativa) has become the main ingredient in natural beauty products such as masks, scrubs, and cold powder. γ-oryzanol and phenolic compounds are the major components in beras that have many benefits, one of which as antioxidants. This study aims to determine the content of γ-oryzanol and total phenolic compounds in different beras cultivars in the area of Yogyakarta. The leveling was done by a TLC-densitometry method and Folin Ciocalteu. The results showed that the largest γ-oryzanol content found in red beras was 0.0843% by weight of the sample. The highest total phenolic content was found in red beras as much as 1.92% by weight of the sample. The antioxidants activity IC50 Red Beras is 0.591.

Keywords


Oryza sativa; γ-oryzanol; fenolik total; antioxidant; Yogyakarta

Full Text:

PDF


References

Aladedunye, F., Przybylski, R., Rudzinska, M., and Pawlik, DK., 2013, γ-Oryzanols of North American Wild Beras (Zizania palustris), J Am Oil Chem Soc, 90(8), 1101-1109

Azlim, A.A.A., Ahmed, J.K.C., Syed, Z.I., Musthapa, S.K., Aisyah, M.R., and Kamarul, R.K., 2010, Total Phenolic Content and Primary Antioxidant Activity of Methanolic and Ethanolic Extracts of Aromatic Plants’ Leaves, International Food Research Journal, 17: 1077-1084.

Butsat, S., and Siriamornpun, S., 2010, Antioxidant Capacities and Phenolic Compounds of the Husk, Bran and Endosperm of Thai Beras, Journal of Food Chemistry 119: 606-613.

Brigitte, K., 1995, Cosmetic sunscreen composition containing ferulic acid and gamma oryzanol, DE Part 442038 (to Gold-well GMBH, DE), Chem Absr, 123, 143661

Choi JS., Jeon, MH., Moon, WS., Moon, JN., Kim, JW, Jung, SK., Ji, YH., Soon, SW., and Kim, MR, 2014, In vivo hair growth-promoting effect of beras bran extract prepared by supercritical carbon dioxide fluid, Biol Pharm Bull, 37(1), 44-53

Chen, M.H., and Bergman, C.J., 2005, A rapid procedure for analyzing beras bran tocopherol, tocotrienol and γ-oryzanol contents, J. Food Comp. Anal., 18: 319-331.

Chun, O.K., Kim, D.O., and Lee, C.Y., 2003, Superoxide Radical Scavenging Activity of The Major Polyphenol in Fresh Plums, J. Food Chem, 51: 8067-8072

Goufo, P., and Trindade, H., 2014, Beras antioxidants: phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, γ-oryzanol, and phytic acid, Food Sci Nutr. 2(2): 75-104

http://terpconnect.umd.edu/~toh/models/BeersLaw.html (dikutip tanggal 20 Januar1 2018) Massarolo, K., Ribeiro, AC., Furlong, EB., and Soares, LAS., 2017, Effect of particle size of beras bran on gamma-oryzanol content and compounds, Journal of Cereal Science, 75, 54-60

Juliano, C., Cossu, M., alamanni, MC., and Piu, L., 2005, Antioxidant activity of gamma-oryzanol: Mechanism of action and its effect on oxidative stability of pharmaceutical oils, International Journal of Pharmaceutic, 299(1-2), 146-154

Patel, M., and Naik, SN., 2004., Gamma Oryzanol from Beras Bran Oil- A Review. Journal of Scientific and Industrial Research, Vol 63, July 2004 : 569-578

Riedel, JH., and Petsitis, X., 1999, Mascara and Eyebrow pencils containing γ-oryzanol and calcium salt, Eur Pat 945120 (toEISAI Co Ltd, JP), Chem Absr, 131, 248032

Senja, R.Y., Issusilaningtyas, E., Nugroho, A.K., and Setyowati, E.P., 2014, The Comparison of extraction Method and Solvent Variation on Yield and Antioxidant activity of Brassica oleracea L. var. capitata f. rubra extract, Traditional Medicine Journal, 19 (1): 43-48

Sutomo, Wahyuono, S., Riyanto, S., and Setyowati, EP., 2013, Isolation and Identification of Active Compound of n-hexane Fraction from Kasturi (Mangifera casturi Konsterm.) against Antioxidant and Immunomodulatory Activity, Journal of Biological Sciences, 13 (7): 596-604

Xu, Z., Hua, N., and Godber, JS., 2001b, Antioxidant Activity of Tocopherols, Tocotrienols, and γ-Oryzanol Components from Beras Bran against Cholesterol Oxidation Accelerated by 2,2‘-Azobis(2-methylpropionamidine) Dihydrochloride, J. Agric. Food Chem., 2001, 49 (4), pp 2077–2081

Xu, Z, Godber, J.S. and Xu, Z., 2001a, Antioxidant activities of major components of γ–oryzanol from beras bran using linolenic acid model, J. Am. Oil Chem. Soc., 78: 465-469.

Zou, Y., Lu, Y., and Wei,D., 2004, Antioxidant activity of Flavonoid-rich extract of Hypericum perforatum L in vitro, J. Agric. Food Chem, 52, 5032-5039.




DOI: https://doi.org/10.22146/mot.33398

Article Metrics

Abstract views : 2903 | views : 4426

Refbacks

  • There are currently no refbacks.




Copyright (c) 2018 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada