Radical Scavenging Activity Combination of Sambiloto (Andrographis paniculata Nees.) and Patikan Kebo (Euphorbia hirta L.) Ethanolic Extracts on 2,2-Diphenyl-1-Picrylhidrazyl (DPPH)

https://doi.org/10.22146/mot.31600

Andayana Puspitasari Gani(1*), Suwidjiyo Pramono(2), Sudibyo Martono(3), Sitarina Widyarini(4)

(1) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(2) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(3) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(4) Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract


Research of radical scavenging activity of sambiloto (Andrographis paniculata Nees) and patikan kebo (Euphorbia hirta L) ethanolic extracts and their combination toward 2,2-Diphenyl-1-Picrylhidrazyl (DPPH) has conducted. Subjects of this experiment comprise sambiloto ethanolic extract, patikan kebo ethanolic extract, dechlorophyllated extract of sambiloto and combinations among those three. Dechlorophyllation by electrocoagulation method is done to reduce chlorophyll content thereby increasing the level of active compound in the sambiloto extract. The results of dechlorophyllation showed that only total flavonoid content in the extract of sambiloto increased, meanwhile the radical scavenging activity results showed that patikan kebo ethanolic extract has the lowest IC50 value (22,36 µg/mL) compared to sambiloto ethanolic extract (IC50 value 499,03 µg/mL). Extract combination may increase DPPH radical scavenging activity of the sambiloto extract (IC50 value 76,06 µg/mL) but not higher than that of patikan kebo single extract.

Keywords


extracts combination; radical scavenging; patikan kebo; sambiloto

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References

Agati, G., Azzarello, E., Pollastri, S., Tattini, M., 2012. Flavonoids as antioxidants in plants: Location and functional significance. Plant Sci. 196, 67–76. https://doi.org/10.1016/j.plantsci.2012.07.014

Jarukamjorn, K., Nemoto, N., 2008. Pharmacological Aspects of Andrographis paniculata on Health and Its Major Diterpenoid Constituent Andrographolide. J. Health Sci. 54, 370–381. https://doi.org/10.1248/jhs.54.370

Jumpatong, K., Phutdhawong, W., Buddhasukh, D., 2006. Dechlorophyllation by Electrocoagulation. Molecules 11, 156–162. https://doi.org/10.3390/11020156

Okhuarobo, A., Ehizogie Falodun, J., Erharuyi, O., Imieje, V., Falodun, A., Langer, P., 2014. Harnessing the medicinal properties of Andrographis paniculata for diseases and beyond: a review of its phytochemistry and pharmacology. Asian Pac. J. Trop. Dis. 4, 213–222. https://doi.org/10.1016/S2222-1808(14)60509-0

Patil, S.B., Nilakwade, N.S., Magdum, C.., 2009. Review on phytochemistry and pharmacological aspects of Euphorbia hirta

linn. Asian J. Pharm. Res. Health Care 1.

Puspitasari, A., 2016. Optimasi Ekstraksi dan Penelusuran Fraksi Aktif Herba Sambiloto (Andrographis paniculata Nees.) dan Patikan Kebo (Euphorbia hirta L.): Studi In Vitro Efek Fagositosis Makrofag. (Disertation). Universitas Gadjah Mada, Yogyakarta.

Puspitasari, A., 2013. Penentuan Kondisi Ekstraksi dan Deklorofilisasi Ekstrak Etanolik Herba Patikan Kebo (Euphorbia Hirta L) Dengan Parameter Kadar Fenolik Total dan Aktivitas Penangkapan Radikal 2-2’ Difenil-1-Pikril Hidrazil (DPPH). Universitas Gadjah Mada, Yogyakarta.

Akowuah, G.A., Zhari, I., Norhayati, I., and Mariam, A., 2006. HPLC and HPTLC densitometric determination of andrographolides and antioxidant potential of Andrographis paniculata. Journal of Food Composition and Analysis, 19: 118–126.

Basma, A.A., Zakaria, Z., Latha, L.Y., and Sasidharan, S., 2011. Antioxidant activity and phytochemical screening of the methanol extracts of Euphorbia hirta L. Asian Pacific Journal of Tropical Medicine, 4: 386–390.

Chairungsi, N., Jumpatong, K., Phutdhawong, W., and Buddhasukh, D., 2006. Solvent effects in electrocoagulation of selected plant pigments and tannin. Molecules, 11: 309–317.

Chang, C., Yang, M.-H., Wen, H.-M., and Chrern, J.-C., 2002. Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. Journal of Food and Drug Analysis, 10: 178–183.

Hossain, M.S., Urbi, Z., Sule, A., and Rahman, H., K.., 2014. Andrographis paniculata (Burm. f.) Wall. ex Nees: A Review of Ethnobotany, Phytochemistry, and Pharmacology. The Scientific World Journal, 2014:1-28

Jumpatong, K., Phutdhawong, W., and Buddhasukh, D., 2006. Dechlorophyllation by electrocoagulation. Molecules, 11: 156–162.

Kumar, S., Malhotra, R., and Kumar, D., 2010. Antidiabetic and free radicals scavenging potential of Euphorbia hirta flower extract. Indian journal of pharmaceutical sciences, 72: 533.

Kurzawa, M., Filipiak-Szok, A., Klodzinska, E., and Szlyk, E., 2015. Determination of phytochemicals, antioxidant activity and total phenolic content in Andrographis paniculata using chromatographic method. Journal of Chromatography B, 995-996: 101–106.

List, P.. dan Schmidt, P., 1989. Phytopharmaceutical Technology. CRC Press, Boston.

Nurfitri and Puspitasari, 2011. 'Deklorofilisasi Ekstrak Etanolik Daun Mangga (Mangifera indica,L) Dengan Metode Elektrokoagulasi', . Presented on Annual IAI National Congress and Science Summit 2011.

Okhuarobo, A., Falodun, J.E., Erharuyi, O., Imieje, V., Falodun, A., dan Langer, P., 2014. Harnessing the medicinal properties of Andrographis paniculata for diseases and beyond: a review of its phytochemistry and pharmacology. Asian Pacific Journal of Tropical Disease, 4: 213–222.

Puspitasari, A., 2013. Penentuan Kondisi Ekstraksi dan Deklorofilisasi Ekstrak Etanolik Herba Patikan Kebo (Euphorbia Hirta L) Dengan Parameter Kadar Fenolik Total dan Aktivitas Penangkapan Radikal 2-2’ Difenil-1-Pikril Hidrazil (DPPH). Research Report, Universitas Gadjah Mada, Yogyakarta.

Ramesh, K. and Padmavathi, K., 2010. Assessment of Immunomodulatory Activity of Euphorbia hirta L. Indian Journal of Pharmaceutical sciences, 72: 621–625.

Rao, K.Y., Vimalamma, G., Venkata Rao, C., and Tzeng, Y.-M., 2004. Flavonoids and andrographolides from Andrographis paniculata. Phytochemistry, 65: 2317–2321.

Saxena, S., Jain, D.C., Gupta, M.M., Bhakuni, R.S., Mishra, H.O., and Sharma, R.P., 2000. High-Performance Thin-Layer Chromatographic analysis of hepatoprotective diterpenoids from Andrographis paniculata. Phytochemical Analysis, 11: 34–36.

Shih, M.-F., Cheng, Y.-D., Shen, C.-R., and Cherng, J.-Y., 2010, A molecular pharmacology study into the anti-inflammatory actions of Euphorbia hirta L. on the LPS-induced RAW 264.7 cells through selective iNOS protein inhibition. Journal of natural medicines, 64: 330–335.



DOI: https://doi.org/10.22146/mot.31600

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