Penares sp sponge from Menjangan Island-water West Bali National Park: Isolation of Cytotoxic Compounds

https://doi.org/10.22146/mot.31547

Erna Prawita Setyowati(1*), Sudarsono Sudarsono(2), Retno Murwanti(3)

(1) Deprtment of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(2) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(3) Department of Pharmacology and Toxicology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract


Sponges are multicellular animals which have cytotoxic compounds. In this study, isolation and cytotoxic activity of Penares sp sponge from Menjangan island-water West Bali National Park have been carried out. The results showed that chloroform extract and GF V Penares sp fraction were toxic according to Brine Shrimp Lethality Test method (each 85% and 63.3% mortality at 25μg/mL concentration). The isolation result of GF V fraction was obtained a compound (Spot 4) that is active as cytotoxic agent against T47D cells (IC50 of 12.7 ug/mL). Spot 4 is a terpenoid compound which has conjugated double bonds.

Keywords


Penares sp; spons; senyawa sitotoksik; sel T47D; sponge; cytotoxic agents; T47D cells;

Full Text:

PDF


References

Carballo, J.L., Inda, Z.L.H., Pérez, P. and Grávalos, M.D.G, 2002, A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products, BMC Biotechnol, 2: 17.

Cragg, GM and Newman, DJ., 2013, Natural Products: A Continuing Source of Novel Drugs Leads, Biochim Biophs Acta, 183(6): 3670-3695

Crews, P., Manes., L.V., and Bohler,M., 1986, Jasplakinolide, a cyclodepsipeptide from the marine sponge Jaspis sp, Tetrahedron Lett. 27(25), 2797-2800

Dias, DA., Urban, S and Roessner, U., 2012, A Historical Overview of Natural Products in Drug Discovery, Metabolites, 2(2), 303-336

Doyle, A., and Griffiths, JB, 1998, Cell and Tissue Culture: Laboratory Procedure in Biotechnology, 1st.Ed.JohnWileyandSonsInc, Chichester, UK.

García, M.M., Valdés, M.D.,Esplá., A.R., Salvador, N., Lopez, P., Larriba, E and Antón, J., 2007, Cytotoxicity of the Ascidian Cystodytes dellechiajei Against Tumor Cells and Study of the Involvement of Associated Microbiota in the Production of Cytotoxic Compounds, Marine Drugs, 5, 52-70

Ghisalberti, E.L., 2008, Detection and Isolation of Bioactive Natural Products, in Colegate, S.M. and Molyneux, R.J. (eds), Bioactive natural Product: Detection, Isolation, and Structural Determination, 2nd ed, CRC Press, New York.

Hooper, J.N.A and Soest, R.W.M. van, 2002, Systema Porifera: A Guide to The Classification of Sponges, Volume I, Kluwer Academic/Plenum Publisher, New York.

Mehbub, M.F, Lei, J., Franco, C and Zhang, W, 2014, Marine Sponge Derived Natural Products between 2001 and 2010, Trends and Opportunities for Discovery of Bioactives, Mar. Drugs., 12, 4539-4577

Meyer, B.N., Ferrigni, N.R., Putman, J.E., Jacobsen, D.E., Nichols, D.E., McLaughlin, J.L., 1982, Brine Shrimp L A Convenient General Bioassay for Active Plant Constituent, Planta Medica, 45, 31-34.

Nakao, Y., Maki, T., Matsunaga, S., van Soest, R.W.M., and Fusetani, N., 2000, Penarolide sulfates A(1) and A(2) new alpha-glucosidase inhibitors from a marine sponge Penares sp. Tetrahedron 56:8977–8987

Samodra, D., 2016, Kanker payudara tertinggi di Indonesia, http://www.antaranew.com/berita/583060/kanker-payudara-tertinggi-di indonesia, tanggal akses 29 Maret 2017

Sawadogo, W.R, Boly, R., Cerella, C., Teiten, M.H., Dicato, M and Diederich, M., A 2015, Survey of Marine Natural Compounds and Their Derivatives with Anti-Cancer Activity Reported in 2012, Molecules 20, 7097-7142

Setyowati, E.P., Jenie, U.A., Sudarsono, Kardono., B., and Rahmat, R, 2008, Identification of cytotoxic constituent of Indonesian sponge Kaliapsis sp. (Bowerbank), J. Pakistan of Biological Sciences 11(22), 2560-2566

Setyowati, E.P., Jenie, U.A., Sudarsono, Kardono., B., Rahmat, R, dan Edy Meiyanto, 2007, Isolation of cytotoxic substance from Kaliapsis sponge, Indonesian Journal of Pharmacy, 18 (4), 183-189

Setyowati, E.P., Sudarsono dan Wahyuono, 2005, Jaspamide: Structure Identification of Cytotoxicity and Fungicide Compound from Stylissa flabelliformis Sponges, Indonesian Journal of Pharmacy, 6 (1), 12-19

Setyowati, E.P., Sudarsono dan Wahyuono, S., 2004, Cytotoxicity and antimicrobial test of the bioactive compound isolated from Stylissa flabelliformis sponge, Indonesian Journal of Pharmacy, 15 (2), 50-56

Wright, 2005, Isolation of Marine Natural Product, In: Natural Product Isolation, Second Edition, edited by Satyajit D. Sarker, Zahid Latif, and Alexander I. Gray, Humana Press, New Jersey, 365-377

Zabrieskie, T.M., Klocke, J.A., Ireland, C.M., Marcus, A.H., Molinski, T.F., Faulkner, J.D., Xu, C., and Clardy, J.C, 1986, Jaspamide, a modified peptide from a Jaspis sponge with insecticidal and antifungal activity, J. Am. Chem. Soc. 108, 3123-3124



DOI: https://doi.org/10.22146/mot.31547

Article Metrics

Abstract views : 279 | views : 352

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 Majalah Obat Tradisional (Traditional Medicine Journal)

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada