Syamsu Nur(1), Rumiyati Rumiyati(2), Endang Lukitaningsih(3*)

(1) Departemen Kimia Farmasi, Fakultas Farmasi Universitas Gadjah Mada Akademi Farmasi Kebangsaan Makassar, Daya, Makassar
(2) Departemen Kimia Farmasi, Fakultas Farmasi Universitas Gadjah Mada
(3) Departemen Kimia Farmasi, Fakultas Farmasi Universitas Gadjah Mada
(*) Corresponding Author


This study aims to determine the bioactivity of L.domesticum as cosmetic ingredient. The extraction was prepared by maceration with ethanol 96 % and ethyl acetate to obtain ethanolic extract of fruit flesh (BLE), ethanolic extract of fruit peel (KLE), ethyl acetate extract of fruit flesh (KLE) and fruit peel (KLEA). Then, determination of compounds was conducted by thin layer chromatography (TLC) and analysed of total phenolic and flavonoid content with colorimetric method. The antioxidant capacity was measured using radical scavenging assay of 1,2-diphenyl-2-picrylhydrazyl (DPPH), β-carotene bleaching assay (BCB) and ferric reducing antioxidant power assay (FRAP). The inhibitory effect of skin degradation enzymes (anti-aging) was carried out using elastase and collagenase assay. The tyrosinase inhibitory effect was determined using mushroom tyrosinase. Based on TLC result, extract of L.domesticum containing phenolic, flavonoids, terpenoids and steroids compounds. The largest of total flavonoid content was obtained in KLE (4,76±0,26 %b/b EQ) and phenolic total content in BLEA (6,4±0,15 %b/b EG). Sequence of antioxidant activity measured using DPPH method was BLEA> KLE> BLE> KLEA; while using BCB method and FRAP method were respectively KLEA>BLEA> KLE> BLE; and BLEA> KLE> BLE> KLEA. KLE and KLEA extracts showed the most potent to remaining activity of the enzyme elastase and collagenase. In addition, BLE extract has the higest tyrosinase inhibition than others. Based on these results it can be concluded that the extracts of the fruit flesh and fruit peel of L. domesticum can be used as a cosmetic active ingredient for anti-aging and anti-tyrosinase.


L.domesticum extract; antioxidants; antiaging; antityrosinase


Chen, Q.-X., Kubo, I., 2002. Kinetics of Mushroom Tyrosinase Inhibition by Quercetin. J. Agric. Food Chem. 50, 4108–4112.

Enzo Life S: Matrix Metalloproteinase-1 (MMP-1) colorimetric drug discovery kit. http / / colorimetric -drug-discovery-kit (accessed on 16 Juni 2016).

Enzo Life Sciences: Neutrophil elastase colorimetric drug discovery kit. (accessed on 16 Juni 2016).

Fremont, lucie, Belguendouz, L., Delpal, S., 1999. Antioxidant activity of resveratrol and alcohol free wine polyphenols related to LDL oxidation and polyunsaturated fatty acids. Life Sci. Elsevier 64, 2511–2521.

Graßmann, J., 2005. Terpenoids as Plant Antioxidants, in: Vitamins & Hormones. Elsevier, pp. 505–535. Hossain, M.A., Shah, M.D., 2011. A study on the total phenols content and antioxidant activity of essential oil and different solvent extracts of endemic plant Merremia borneensis. Arab. J. Chem. Prod. Hosting Elsevier BV 8, 66–71.

Jia, N., Li, T., Diao, X., Kong, B., 2014. Protective effects of black currant (Ribes nigrum L.) extract on hydrogen peroxide-induced damage in lung fibroblast MRC-5 cells in relation to the antioxidant activity. J. Funct. Foods 11, 142–151.

Karim, A.A., Azlan, A., Ismail, A., Hashim, P., Gani, S. salwa abd, Zainudin, B.H., Abdullah, N.A., 2014. Phenolic composition, antioxidant, anti-wrinkles and tyrosinase inhibitory activities of cocoa pod extract. BMC Complement. Altern. Med. 14, 381.

Kim, D.-B., Shin, G.-H., Kim, J.-M., Kim, Y.-H., Lee, J.-H., Lee, J.S., Song, H.-J., Choe, S.Y., Park, I.-J., Cho, J.-H., Lee, O.-H., 2016. Antioxidant and anti-ageing activities of citrus-based juice mixture. Food Chem. 194, 920–927.

Kim, Y.H., Chung, C.B., Kim, J.G., Ko, K.I., Park, S.H., Kim, J.-H., Eom, S.Y., Kim, Y.S., Hwang, Y.-I., Kim, K.H., 2008. Anti-wrinkle activity of ziyuglycoside I isolated from a Sanguisorba officinalis root extract and its application as a cosmeceutical ingredient. Biosci. Biotechnol. Biochem. 72, 303–311.

Klungsupya, P., Suthepakul, N., Muangman, T., Rerk-Am, U., Thongdon-A, J., 2015. Determination of Free Radical Scavenging, Antioxidative DNA Damage Activities and Phytochemical Components of Active Fractions from Lansium domesticum Corr. Fruit. Nutrients 7, 6852–6873.

Kulisic, T., Radonic, A., Katalinic, V., Milos, M., 2004. Use of different methods for testing antioxidative activity of oregano essential oil. Food Chem. 85, 633–640.

Lin, J.-W., Chiang, H.-M., Lin, Y.-C., Wen, K., 2008. Natural products with skin-whitening effects. J. Food Drug Anal. 16. Lukitaningsih, E. and Holzgrabe, U., 2014. Bioactive compounds in bengkoang (Pachyrhizus erosus) as antioxidant and tyrosinase inhibition agents. Indonesia J. Pharm. 25(2), 68-75

Nishizawa, M., Emura, M., Yamada, H., Shiro, M., Chairul, Hayashi, Y., Tokuda, H., 1989. Isolation of a new cycloartanoid triterpene from leaves of Lansium domesticum novel skin-tumor promotion inhibitors. Tetrahedron Lett. Elsevier Ltd 30, pp 5615–5618.

Nishizawa, M., Nishide, H., Hayashi, Y., 1982. The Structure of Lansioside A: A Novel Triterpene Glycoside with Amino-Sugar from Lansium Domesticum. Tetrahedron Lett. Elsevier Ltd 23, 1349-1350.

Pokorny, J. (Ed.), 2003. Chapter 1. Antioxidants in food: practical applications, Reprint. ed, Woodhead publishing in food science and technology. Woodhead, Cambridge. pp 42-50

Pouillot, A., Polla, L.L., Tacchini, P., Neequaye, A., Polla, A., Polla, B., 2011. Natural antioxidants and their effects on the skin. Formul. Packag. Mark. Nat. Cosmet. Prod. Ed. Dayan N Kromidas L.

Sahasrabudhe, A., Deodhar, M., 2010. Anti-hyaluronidase, anti-elastase activity of Garcinia indica. Int. J. Bot., 3 6, 299–303. Singleton, V.L., Orthofer, R., Raventos, L., 1999. Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. Methods Enzymol. 299, 119.

Tanaka, T., Ishibashi, M., Fujimoto, H., Okuyama, E., Koyano, T., Kowithayakorn, T., Hayashi, M., Komiyama, K., 2002. New Onoceranoid Triterpene Constituents from Lansium d omesticum. J. Nat. Prod. 65, 1709–1711.

Thring, T.S., Hili, P., Naughton, D.P., 2009. Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants. BMC Complement. Altern. Med. 9.

Tilaar, M., Wih, W.L., Ranti, A.S., Wasitaatmadja, S.M., Junardy, F.D., others, 2008. Review of Lansium domesticum Corrêa and its use in cosmetics. Bol Latinoam Caribe Plant Med Aromat. 7, 183–189.

Vaya, J., Aviram, M., 2001. Nutritional antioxidants mechanisms of action, analyses of activities and medical applications. Curr. Med. Chem.-Immunol. Endocr. Metab. Agents 1, 99–117.

Wiedow, O., Schröder, J.M., Gregory, H., Young, J.A., Christophers, E., 1990. Elafin: an elastase-specific inhibitor of human skin. Purification, characterization, and complete amino acid sequence. J. Biol. Chem. 265, 14791–14795.

Wong, S.K., Lim, Y.Y., Chan, E.W.C., 2010. Evaluation of antioxidant, anti-tyrosinase and antibacterial activities of selected Hibiscus species. Ethnobot. Leafl. 2010, 9.


Article Metrics

Abstract views : 8905 | views : 43567


  • There are currently no refbacks.

Copyright (c) 2017 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada