IMMUNOMODULATOR EFFECT OF COMBINATION OF Andrographis paniculata (Burm. f.) Nees HERB AND GINGER RHIZOME (Curcuma xanthorrhiza Roxb.) ETHANOLIC EXTRACT ON CELL PROLIFERATION OF Balb/c MICE LYMPHOCYTES IN VITRO

https://doi.org/10.22146/tradmedj.17322

Damriati Azimah(1), Yuswanto Yuswanto(2), Wahyono Wahyono(3*)

(1) Department of Biology, Faculty of Pharmacy, Universitas Gadjah Mada
(2) Department of Biology, Faculty of Pharmacy, Universitas Gadjah Mada
(3) Department of Biology, Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Our susceptibility to various infectious diseases can be avoided by increasing the specific immune responses either by the proliferation of lymphocytes. Immunomodulator effect of Andrographis paniculata (Burm.f.) Nees) and Curcuma xanthorrhiza Roxb have been well evaluated. Empirically, the East Borneo’s tribe combined both herbs to treat jaundice disease. However, scientific evidence is still needed in their use as a drug candidate. This study explores further effect of the immunomodulator from 5 ethanol extract groups. Quantitative analysis was also completed using a densitometer to obtain andrographolide and curcumin levels of ethanol extract. Lymphocyte proliferation was tested by using MTT colorimetric method, the results was read by an ELISA reader and statistically analyzed with One-Way ANOVA test with 95 % of confidence level. The maceration yield of sambiloto ethanol extract (EES) and temulawak ethanol extract (EET) were respectively 5.78 % w / w and 3.92 % w / w. The results of quantitative analysis in 28.6 mg EES contained 11.43 % andrographolide and from the 251.8 mg EET contained 28.79 % curcumin. From the MTT test, all treatment groups were significantly different from the control group. Based on the OD values, the best combination to increase lymphocyte cell proliferation ETS was a group contained 56.25 mg of temulawak and 18.75 mg of sambiloto in 1 ml of solvent. Nonetheless, the value was not higher than the ability of ET in increasing cell proliferation.

Keywords


Imunomodulator, Sambiloto, Temulawak, TLC, Lymphocyte Proliferation



References

Abbas, A. K., Litchman, A. H., & Pillai, S. (2012). Cellular and Molecular Immunology, 7th Ed. Philadelphia: Saunders Elsevier.

Badan POM RI. (2011). Formularium Ramuan Obat Tradisional Indonesia Ramuan Etnomedisin vol. I. Jakarta: CV GLOBAL exPRESS media.

Barata, Ketut,. (2009). Mencit Balb/C Dapat Digunakan Sebagai Hewan Model Penelitian Virus Penyakit Jembrana. Bali: Buletin Veteriner Udayana.

Baratawidjaja, K. G. (2000). Imunologi Dasar, Edisi Keempat. Jakarta: Fakultas Kedokteran Universitas Indonesia.

Bermawie, N., Rahardjo, M., Wahyuno, D., & Ma’mun. (2006). Status Teknologi Budidaya dan Pascapanen Tanaman Kunyit dan Temulawak Sebagai Penghasil Kurkumin. Balitro. 2(4). 84-99.

Chao, W. W., & Lin, B. F. (2010). Isolation and Identification of Bioactive Compounds in Andrographis paniculata (Chuanxinlian). Journal Chinese Medicine.

Delves, P. J., and Roitt., I.M. (2000). The Immune System; First of Two Parts, 37, 42. The New England Journal of Medicine , Volume 343 Numb 1.

Mosmann, Tim,. (1983). Rapid Colorimetric Assay for Celluler Growth and Survival : Application to Proliferation and Cytotoxicity Assays. Journal of Immonological Methods 65.

Gritter, R. J., Bobbit, J. M., & Schwarting, A.E., (1985). Pengantar Kromatografi Edisi Kedua. diterjemahkan oleh Padmawinata, K. 1991. Bandung: Penerbit ITB.

Kurniawan, A. (2011). Aktivitas Antioksidan dan Potensi Hayati dari Kombinasi Ekstrak Empat Jenis Tanaman Obat Indonesia. Bogor: Bogor Agricultural University Press.

Kresno, S. B. (2001). Imunologi : Diagnosis dan Prosedur Laboratorium. Edisi IV. Jakarta: Fakultas Kedokeran Umum Universitas Indonesia.

Spelman, K., J. J. Burns, D. Nihols, N. Winters, S. Otterberg, & M. Tenborg. (2006). Modulation of cytokine expression by traditional medicines: a review of herbal immunomodulator. Alternative medicine review. 2006 Juni : II (2). pp. 128-150.

Trubus. (2010). Herbal Indonesia Berkhasiat Bukti ilmiah & Cara Racik Vol. 08. Bogor: Trubus Info kit.

Wagner, H., & Bladt, S. (1984). Plant Drug Analysis; A Thin Layer Chromatography Atlas. Berlin : Springer-Verlag, 1984. hal. 299.

Wang, K., Saito,S., Bisikirska, B., Alvarez,M., Lim, M., Rajbhandari, P.,Shen, Q., Nemenman, Basso,K., Margolin,A., Klein,U., Dalla-Favera, R., & Califano. (2009). Genome-wide identification of post-translational modulators of transcription factor activity in human B cells. Nature Biotech. 27:829.

Widianto, M. B. (1987). Immunomodulator. Bandung: Institut Teknologi Bandung Press.



DOI: https://doi.org/10.22146/tradmedj.17322

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