ANTIHYPERTENSIVE TESTING OF COMBINATION OF Apium graveolans L., Orthosiphon stamineus Benth., AND Morinda citrifoliaL EXTRACT. ON NORMOTENSIVE AND HYPERTENSIVE SPRAGUE DAWLEY RATS

https://doi.org/10.22146/tradmedj.17321

Rumiyati Rumiyati(1*), Arif Rahman Hakim(2), Arini Dwi Winarti(3), Dea Nurma Septia(4)

(1) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
(4) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


Seledri (Apium graviolens L.), kumis kucing (Orthosiphon stamineus Benth.), and mengkudu (Morinda citrifolia L.) have been used to treat hypertension diseases in Indonesia. Previous researches have been done to determine antihypertensive activity of extracts of the sample in single extract. However, there are limited studies on effect of the extracts on a combination of three samples. This research was therefore aimed to study the effect of the combination on lowering blood tension on normotensive and spontaneously hypertensive rats. Non Invasive Blood Pressure technique equipped with Non Invasive Blood Pressure System (CODA®) was used to measure effect of extracts on antihypertensive activity. Sprague Dawley rats were divided into 2 groups (normotensive and spontaneously hypertensive rats using fenilefrin) and treated with combination extracts in doses of 20.25 and 40.5 mg/kg BW of rats. The result showed that the combined extracts can decrease in blood pressure of both two of the groups. Normotensive group of the rats treated at doses of 20,25 and 40,5 mg/kg BW of rats had lower persentase of systolic blood pressure as much as 7,1 ± 1,8 % and 10,2 ± 2,6 %, whilst percentage of diastolic blood pressure had lower percentage as much as 13,8 ± 3,2 % and 12,3 ± 3,1 %, respectively. Furthermore, spontaneously hypertensive rats treated at doses of 20,25 and 40,5 mg/kg BW of rats also showed lower systolic blood pressure as much as 16,10 ± 0,90 % and 15,84 ± 1,55 % and diastolic blood pressure as much as 19,48 ± 1,03 % and 17,77 ± 1,34 %. These results suggest that administration of combination of three extracts exhibited antihypertensive in both of the rat groups.

Keywords


A graviolens L, O stamineus Bent, M citrifolia L. and Non Invasive Blood Pressure



References

Arafat, O.M., Tham, S.Y., Sadikun, A., Zhari, I., Haughton, P.J., and Asmawi, M.Z., 2008.udies on Diuretic and Hypouricemic Effect of Orthisiphon stamineus methanol extracts in Rats, Journal of Ethnopharmacology, 118, 354-364

Aceves-Avila, F.J, Medina, F., & Fraga, A., 2001, Herbal Therapies in Pharmacology: The Persistence of Ancient Medical Practices, Clinical and Experimental Rheumatology, 19, 177-183.

Almatar, M., Ekal, H.& Rahmat, Z., 2014, A Glance Applications of Orthosiphon stamineus and Some of its Oxidative Compounds, International Journal of Pharmaceutical Sciences Review and Research, 24(2), 84.

Anonim, 2013, A Global Brief on Hypertension, 17, WHO Press, Geneva.

Chan, E.C., Pannahpech, P.& Woodman, O.L., Relaxation to Flavones and Flavonols in Rat Isolated Thoracic Aorta: Mechanism of Action and Structure-Activity Relationship, Journal of Cardiovascular Pharmacology, 5, 326-333.

Gharouni, M.& Sarkati A., 2000, Application of Apium graveolens in Treatment of Hypertension, Journal of Tehran University of Medical Sciences, 3, 67–69.

Jorge, V.G., Angel, J.R. L, Adrian, T.S., Francisco, A.C., Anuar, S.G., Angel, S.O. and Emmanuel, H.N., 2013, Vasorelaxant Activity of extracts obtained from Apium graviolens: Possible Sourcr for Vasorelaxant Molecul Isolation with Potential Antihypertensive effect, Asian Pasific Journal of Tropical Biomedicine, 3 (10), 776-779

Kumar, R., Kumar, A., Sharma, R.& Baruwa, A., 2010, Pharmacological Review on Natural ACE Inhibitors, Der Pharmacia Lettre, 2(2), 286.

Navarro, V., Villarreal, M.L., Rojas, G., & Lozoya, X., 1996, Antimicrobial Evaluation of Some Plants Use in Mexicantraditional Medicine for Treatment of Infectious Diseases, Journal of Ethnopharmacology,53, 143-147.

Nayak, S. & Shettigar, R., 2010, Morinda citrifolia: a Review, Journal of Pharmacy Research, vol. 3, no. 8, 1872-1874.

Okeke, M.I., Iroegbu, C.U., Eze, E.N., Okolali A.S., & Esimone, C.O., 2001, Evaluation of Extracts of the Root of Landolphia Owerrience for Antimicrobial Activity, Journal of Ethnopharmacology,78, 119-127.

Rapavi, E., Blazovics, A., &Fehr, J., 2000, Therapeutic Herbs in Ancient Chinese Medicine, Hungarian Medical Journal,141, 2093-2096.

Rawat, P., Singh, P.K. & Kumar, V., 2016, Anti-hypertensive medical plants and their mode of action, Journal of Herbal Medicine, Vol. 6, No. 3, 107-118

Rumiyati, Sudarsono, Susanto, B.O., Mayasari, S.K., Wijaya, R.N, 2016, In vitro Angiotensin Converting Enzyme (ACE) inhibitory assay of extracts containing phenolic compounds of edible plants, International Journal of Pharmaceutical and Clinical Research, 2016; 8(5) Suppl: 414-418

Syarif, A., Estningtyas, A., Setiawati, A., Muchtar, A., Arif, A., Bahry, B., Suyatna, F., D., Dewoto, H., R., Utama, H., Darmansjah, I., Wiria, M, S.,S., Nafrialdi, Wilmana, P. F., Ascobat, P, Setiabudy, R.,Sunaryo, R., Wardhani, S., Suherman, S., K., Ganiswara, V., H., Arozal, W., Mariana, Y., H., Sadikin, Z., D., dan Louisa, M.,2007, Farmakologi dan Terapi, 354-356, Balai Penerbit FKUI, Jakarta

Sherma, J. & Fied, B., 2003, Handbook of Thin-Layer Chromatography, 3rd Edition, Marcel Dekker, New York.

Yamaguchi, S., Ohnishi, J., Sogawa, M., Maru, I., Ohta, Y. & Tsukada, Y., 2002, Inhibition of Angiotensin I Converting Enzyme by Noni (Morinda citrifolia) Juice, Nippon Shokuhin Kagaku Kogaku Kaishi, Vol 49, No 9, 625-626.

Yang, S.C., Chen, T.I., Li, K.Y.& Tsai, T.C., 2007, Change in Phenolic Compound Content, Reductive Capacity and ACE Inhibitory Activity in Noni Juice during Traditional



DOI: https://doi.org/10.22146/tradmedj.17321

Article Metrics

Abstract views : 1361 | views : 17509

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada