AN IN VITRO ANTIOSTEOPOROTIC ACTIVITY OF 96% ETHANOL EXTRACT OF Abelmoschus manihot L. MEDIK LEAVES USING MC3T3-E1 PREOSTEOBLAST CELLS

https://doi.org/10.22146/tradmedj.17297

Agnis Pondinekaria Aditama(1*), Mangestuti Agil(2), Hening Laswati(3)

(1) Faculty of Pharmacy, Universitas Airlangga
(2) Faculty of Pharmacy, Universitas Airlangga
(3) Faculty of Pharmacy
(*) Corresponding Author

Abstract


Osteoporosis is a disease characterized by low bone mass and structural deterioration of bone tissue. Estrogen deficiency causes loss of bone mineral density which causes osteoporosis. Phytoestrogen is a potential alternative of estrogen that can be used as Hormone Replacement Therapy on osteoporosis with minimum side effects. Many edible plants contain phytoestrogens that are believed to promote bone health. Abelmoschus manihot (L.) Medik has been known as a plant that is empirically used in the traditional medicine and has potency to prevent osteoporosis. The aim of this research is to determine whether the Abelmoschus manihot (L.) Medik leaves have the potential to increase bone formation in an in vitro assays using preosteoblast cell line MC3T3-E1. The results showed an increasing activity of Alkaline phosphatase using confocal laser scanning microscopy technique.

Keywords


Abelmoschus manihot L. Medik, preosteoblast MC3T3-E1 cell, alkaline phosphatase, in vitro.



References

Badole S and Kotwal S. 2014. Equisetum arvense: Ethanopharmacological and Phytochemical review with reference to osteoporosis. RTM Nagpur University. Issue 4, Vol 1.

Branca F. 2003. Dietary Phyto Oestrogens and Bone Health. Proceeding of the Nutrition Society, 877-887.

Brotosudirdjo M. 2000. Telaah fitokimia daun Gedi merah (Abelmoschus manihot L. Medik), Malvaceae. Dalam : Penelitian

Tanaman Obat Di Beberapa Perguruan Tinggi di Indonesia. Buku X. Jakarta: Depkes RI. Balitbang Kesehatan Puslitbang Farmasi.

Chen, L., Lei, L., Ding, P., Tang, Q., Wu, Y. 2011. Osteogenic effect of Drynariae rhizoma extracts and Naringin on MC3T3-E1 cells and an induced rat alveolar bone resorption model. Elsevier : Archieves of oral biology. Vol 56. Page 1655-1662.

Choi, E. M. 2012. Liquiritigenin isolated from Glycyrrhiza uralensis stimulates osteoblast function in osteoblastic MC3T3-E1 cells. Elsevier : International Immunopharmacology. Vol 12. Page 139-143.

Fujiwara, K., Shin, M., Hougaard, D. M., Saita, T. Distribution study of peplomycin in rat kidney revealed by immunocytochemistry using monoclonal antibodies. Histochem Cell Biology. Vol 135. page 93-101.

Glover A and Assinder S. 2006. Acute exposure of adult male rats to dietary phytoestrogen reduces fecundity and alters epididymal steroid hormon receptor expression. Jour. Endoc. 189: 565-573.

Grippo A, Capps K, Rougeau B and Gurley BJ. 2007. Analysis of flavonoid phytoestrogens in botanical and ephedra-containing dietary

supplements. Ann Pharmacother. 41:1375–82.

Jain P and Bari S. 2009. Isolation of Stigmaterol and γ-sitosterol from petroleum ether exstract of woody stem of Abelmoschus manihot. Asian Journal of Biological Sciences.

Jefferson W, Padilla-Banks E, Clark G and Newbold R. 2002. Assessing estrogenic activity of phytochemicals using transcriptional activation and immature mouse uterotrophic responses. Journal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences 777(1-2):179-189.

Jia L and Guo Li M. 2011. Chemical constituents from petroleum ether portion of Abelmoschus esculentus II. China journal of chinese Materia Medica.

Jia L and Guo Li M. 2011. Chemical constituents from petroleum ether portion of Abelmoschus esculentus II. China journal of chinese Materia Medica.

Kovalchuk S, Kozhemyako, V, Atopkina S, Silchenko A, Avilov S, Kalinin V, Rasskazov V and Aminin D. 2006. Estrogenik activity of triterpene glycosides in yeast two-hybrid assay. The Journal of Steroid Biochemistry and Molecular Biology. Volume 101. Issue s

– 5. Pages 226 – 231.

Lai X, Liang H, Zhao Y and Wang B. 2009. Simultaneous determination of seven active flavonols in the flowers of Abelmoschus manihot by HPLC. J. Chromatogr. Sci. 3, 206–210.

Liu Y, Xianyin L, Xiaomei, yuying Z and Jingrong C. 2006. Interactions Between Thrombin with Flavonoids from Abelmoschus manihot Medicus by CZE. Chromatographia.

Mamahit L. 2009. Satu Senyawa Steroid Dari Daun Gedi (Abelmochus manihot L. Medik) Asal Sulawesi Utara. Chem. Prog. Vol. 2, No 1.

Puel C, Mathey J, Davicco M, Labecque P, Chanteranne B, Horcajada M and Coxam V. 2005. Preventive effect of Abelmoschus

manihot L. Medik on bone loss in the ovariectomised rats. J Ethnopharmacology. 99:655-660.

Taylor, C. R., and Rudbeck, L. 2013. Immunohistochemical Staining Methods. Dako Denmark : IHC Handbook sixth edition.

Urasopon N, Hamada Y, Cherdshewasart W, Malaivijitnond S. 2008. “Preventive effects of Pueraria mirifica on bone loss in

ovariectomized rats,” Maturitas, vol. 59, no. 2, pp. 137–148.

Vandenberg, L. N., Colborn, T., Hayes, T. B., Heindel, J. J., Jacobs, D. R., Lee, D-H., Shioda, T., Soto, A. M., Saal, F. S., Welshons, W. V., Zoeller, R. T., Myers, J. P. 2012. Review : Hormones and Endocrine-Disrupting Chemicals: Low-Dose Effects and Nonmonotonic Dose Responses. Endocrine Journal. Vol 33. No 3.

Widyowati, R. 2011. Alkaline Phosphatase Activity of Graptophylum pictum and Sphilanthes acmella Fractions Against MC3T3-E1 Cells

as Marker of Osteoblast Differentation Cells. International Journal of Pharmacy and Pharmaceutical Sciences. Vol 3. Suppl 1.

Yamaguchi M, Lai Y 2006. Oral administration of phytocomponent p-hydroxycinnamic acid has anabolic effects on bone calcification in

femoral tissues of rats in vivo. J Health Sci 52; 308 – 312.

Yang Y, Zhang Z, Li S, Ye X, Li X, He K. 2014. Synergy effects of herb extracts: Pharmacokinetics and pharmacodynamic basis. Fitoterapia. Vol 92, P 133–147



DOI: https://doi.org/10.22146/tradmedj.17297

Article Metrics

Abstract views : 479 | views : 1218

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada