DETERMINATION OF ANTIOXIDANT ACTIVITIES OF BUAS-BUAS LEAVES (Premna serratifolia L.) USING DPPH (2,2-diphenyl-1-picrylhydrazyl) METHOD

https://doi.org/10.22146/tradmedj.17292

Isnindar Isnindar(1*), Subagus Wahyuono(2), Sitarina Widyarini(3), Yuswanto Yuswanto(4)

(1) Faculty of Medicine, Study Program of Pharmacy, Unversitas of Tanjung Pura
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Animal Medicine, Universitas Gadjah Mada
(4) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Antioxidant is a substance that in small concentrations can significantly inhibit or prevent oxidation on the substrate. Buas-buas (Premna serratifolia L.) is one of the plants that have antioxidant effect. The antioxidant activities of the wasbenzen and ethanol extracts of buas-buas leaves were determined by DPPH method using spectrophotometer UV-Vis. Data resulted from the analysis of antioxidant activity (IC50) is 532.24 μg/mL of wasbenzen extract, 24.40 μg/mL of ethanol extract, and 1.83 μg/mL of vitamin C.


Keywords


Buas-buas leaves; Premna serratifolia L; wasbenzen extract; ethanol extract; antioxidant activity; DPPH method

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References

Ali, M.S.M. 2008. Analysis of Phenolics and Other Phytochemicals in Selected Malaysian Traditional Vegetables and Their Activities In Vitro. Tesis. University of Glasgow, for the degree of Doctor of Philosophy (PhD) Malaysia. Asharani, I.V., Thirumalai, D., Paridhavi, M., Gowtham, M. 2013. Physiochemical, Pharmacognostical and Phytochemical Evaluation of Premna latifolia. International Journal of Pharmacy and Pharmaceutical Sciences. Vol 5. Issue 4. 2013. India. Bakar, F.A., Mohamed, S., Hamid, A.A., dan Mustafa, R.A. 2010. Total Phenolic Compounds, Flavonoids, And Radical Scavenging Activity Of 21 Selected Tropical Plants. Journal Of Food Science. Malaysia. Bose, L.V., Varghese, G.K., and Habtemariam, S. 2013. Identification of Acteoside as the active antioxidant principle of Premnaserratifolia root wood tissues. Phytopharmacology. 2013. 4(2). 228-236. Gulcin, I., Uguz, M.T., Oktay, M., Beydemir, S. and Kufrevioglu, O.I. 2004. Evaluation of the antioxidant and antimicrobial activities of clary sage (Salvia sclarea L.), Turki. I. Agric. For. 28: 25-33. Halliwel, B. and Gutteridge, J.M.S. 1999. Free radicals in biology and medicine, 3th ed. Pp. 1-231, 353-425.Oxford University Press.Inc. New York. Jang, C.I., Jo, K.E., Bae, S.M., Lee, J.H., Jeon, I.G., Park, E., Yuk, G.H., Ahn, H.G., Lee, C.S. 2010. Antioxidant and antigenotoxic activities of different part of persimmon (Diospyros kaki cv fuyu) fruit. J. Medicinal Plants Res. (4) 2. Pp: 155-160. Kaur, A., Nain, P, Nain, J. 2012, Herbal Plants Used In Treatment of ReumatoidArtritis : A Review. International Journal Of Pharmacy and Pharmaceutical Sciences.Vol.4 Suppl 4. Kwon, Y.S. and Kim, C.M. 2003. Antioksidant constituents from the steam of sorghum bicolor. Arch. Pharm. Res. 26 (7): 535-539. Maslarova, N.V.Y. 2001. Inhibiting oxidation cited in Pokorny J, Yanishlieva N and Gordon M. Antioxidant in food. Practical Applications.42-48. CRC Press. New York. Oke, J.M. dan Hamburger, M.O. 2002. Screening of some nigerian medicinal plants for antioxidant activity using 2,2, diphenylpicryl-hidrazyl radical. AJBR. 5: 77-79. Rajendran, R., Krishnakumar, E. 2010. Antiarthritic activity of Premnaserratifolia Linn., wood against adjuvant induced arthritis. Avicenna Journal of Medical Biotechnology 2, 101–106. Vadivu, R., Suresh, A. J., Girinath, K., Kannan, P. B., Vimala, R., dan Kumar, N. M. S. 2008. Evaluation of Hepatoprotective and In-vitro Cytotoxic Activity of Leaves of Premna serratifolia Linn. Journal of Scientific Research Publications. Department of Pharmacognosy, College of Pharmacy, Madras Medical College, Chennai-03, India. Yadav D, Nusrat M, Suaib L, Pemaiah B, Madan M. Gupta. 2012, Antioxidant furofuranlignans from Premnaintegrifolia, Elsevier B.V. All rights reserved. Yagi, A., Kabash, A., Okamura, N., Haraguchi, H., Moustafa, S.M. and Khalifa, T.I. 2002. Antioxidant, free radical scavenging and anti inflamatory effect of aloesin derivatives in Aloe vera.Planta Med. 68: 957-960.



DOI: https://doi.org/10.22146/tradmedj.17292

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