Piper betle LEAVES EXTRACT PATCH: EVALUATION OF ANTIBACTERIAL ACTIVITY, RELEASE PROFILE OF EUGENOL, AND LOCAL TOLERANCE

https://doi.org/10.22146/tradmedj.12825

Mufrod Mufrod(1*), Suwaldi Suwaldi(2), Subagus Wahyuono(3)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Piper betle leaf in extract form is more effective than crude drug. Eugenol is a component in the extract that has antibacterial activity but irritate. Patch of piper betle leaf extract was used on the mucosa to make oral cavity hygiene. Antibacterial activity was influenced by the release of eugenol from the patch. Release enhancer substances (RES) such as glycerin, propylen glicol and tween 80 were added in patch formulation to increase the release of active substances. The aim of the research was to investigate the physicochemical properties, eugenol release profiles, and local tolerance test of the patch. Extract of piper betle leaf was made using infundation method. Patch was made according to the variation concentration of extract (1, 2 and 4%) and RES (glycerine, propylen glycol and tween 80) using chitosan as vehicle. Patch produced solvent casting method. Patch obtained was tested for swelling index, folding endurance, surface pH, antibacterial activity, release of eugenol, and local tolerance. Data obtained were analyzed descriptively. The result showed that the addition of RES did not affect the surface pH but increase the water absorption with in inconsistent way except patch with tween 80. The flexibility (folding endurance value) increased, and the highest amount of eugenol released was achieved by patch using propylen glicol. Patch with tween 80 and glycerin for all extract concentration and patch with 1% extract concentration using propylen glycol showed medium sensation (local tolerance), and patch with 2 and 4% extract using propylen glycol showed severe sensation.

Keywords


piper betle extract, patch extract, release enhancer substances, local tolerance



References

Adriaens, E., 2000, De Ontwikkeling van een Alternatieve Mucosale Irritatietest. Thesis, Ghent University, Belgium, 129 cit. Dhondt, M., 2005, Optimization and Validation of An Alternative Mucosal Irritation Test, Thesis, Faculty of Pharmaceutical Sciences, Universiteit Gent

Adriaens, E., 2006, The Slug Mucosal Irritation Assay: An Alternative Assay for Local Tolerance Testing, National Center for the Replacement, Refinement and Reduction of Animals in Research, 8, 1-9

Aulton, M.E., 2007, The Design and Manufacture of Medicine, NY.

Deshmane, S.V., Channawar, M. A., Chandewar, A.V., Joshi, U. M. & Biyani, K.R., 2009, Chitosan Based Sustained Release Mucoadhesive Buccal Patches Containing Verapamil HCl, International Journal of Pharmacy and Pharmaceutical Science, 1 (1), 216-229

Figuer Figueiras, A., Pais, A. A. C. C., & Veiga, F. J. B., 2010, A Comprehensive Development Strategy in Buccal Drug Delivery, AAPS Pharm.Sci.Tech, 2(4), 1703-1712

Hidayaningtyas, P., 2008, Perbandingan Efek Antibakteri Air Seduhan Daun Sirih (Piper betle Linn) terhadap Streptococcus mutans pad Waktu Kontak dan Konsentrasi yang Berbeda, Skripsi, Fakultas Kedokteran Universitas Diponegoro Semarang

Kaur, A., Kaur, G.,2012, Mucoadhesive buccal patches based on interpolymer complexes chitosan-pectin for delivery of carvedilol, Saudi Pharmaceutical Journal, 20,21-27

Kumar, A., Phatarpekar, V., Pathak, N., Padhee K., Garg, M., and Sharma N., 2011, Formulation development and evaluation of carvedilol bioerodable buccal mucoadhesive patches, International Journal of Comprehensive Pharmacy, 3(07), 1-5.

Lenoir, J., Veryser,L., Boonen, J., Brocke, N., Wynendack, E., Adraens, E., Nelis, H.,Wever, B.D., Remon, J.P., and Spiegeleer, B.D., 2015, Evaluation of Local Tolerance of a plant extract by the slug mucosal irritation (SMI) assay, J.tox.ed, 3, 1-12

Mitsui, T.,(ed.),1998, New Cosmetic Science, 132-135, Elsevier,NY.

Nash, R. A. & Wachter, A. H., Pharmaceutical Process Validation, an International Third Edition, 551-556, Marcel Dekker, New York

Niazi, S. K., 2009, Handbook of Pharmaceutical Manufacturing Formulation Liquid Products, 2nd Ed., 52, Informa Healthcare, New York

Patel V.M., Prajapati, B.G., and Patel, M.M., 2009, Design and in vitro characterization of eudragit containing mucoadhesive buccal patches, International Journal of PharmTech Research, 1(3), 783-789

Rowe, R. C., Sheskey P. J., & Quinn M. E., 2009, Handbook of Pharmaceutical Excipient, VI Ed., 5-6, 159-162, 517-522, 549-553, 283-286, Pharmaceutical Press, London

Seedher N, and Agarwal P., 2009, Various solvent systems for solubility enhancement of enrofloxacin, Indian J.Pharm .Sci, 71(1), 82-87.

Towaha, J., 2012, Manfaat Eugenol dalam Berbagai Industri di Indonesia, Perspektif, 11 (2), 79-90



DOI: https://doi.org/10.22146/tradmedj.12825

Article Metrics

Abstract views : 885 | views : 1832

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada