Burhan Ma'arif(1*), Mangestuti Agil(2), Hening Laswati(3)

(1) Department of Pharmacognocy and Phytochemistry, Faculty of Pharmacy, Airlangga University
(2) Department of Pharmacognocy and Phytochemistry, Faculty of Pharmacy, Airlangga University
(3) Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Airlangga University
(*) Corresponding Author


Estrogen deficiency causes various health problems in postmenopausal women, including osteoporosis. Phytoestrogen emerged as a potential alternative of estrogen with minimum side effects. Green clover (Marsilea crenata Presl.) is a typical plant in East Java which suspected contains estrogen-like substances. The aim of this research was to report the phytochemical properties of M. crenata using GC-MS as a preliminary study. M. crenata leaves were dried and extracted with n-hexane, then separated using vacuum column chromatography to get four fractions, after that the n-hexane extract and four fractions were identified with GC-MS. The results of GC-MS analysis showed some compounds contained in M. crenata leaves like monoterpenoid, diterpenoid, fatty acid compounds, and other unknown compounds. The results obtained in this research indicated a promising potential of M. crenata as medicinal plants, especially as antiosteoporotic agent.


Marsilea crenata Presl., phytochemical, antiosteoporosis, GC-MS

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