IN VITRO ANTHELMINTIC ACTIVITY OF Veitchia merrillii NUTS AGAINST Ascaridia galli

https://doi.org/10.22146/tradmedj.12818

Abdullah Hamzah(1), Muhammad Hambal(2), Ummu Balqis(3*), Darmawi Darmawi(4), Maryam Maryam(5), Rosmaidar Rosmaidar(6), Farida Athaillah(7), Muttaqien Muttaqien(8), Azhar Azhar(9), Ismail Ismail(10), Rastina Rastina(11), Eliawardani Eliawardani(12)

(1) Faculty of Veterinary Medicine, Syiah Kuala University
(2) Faculty of Veterinary Medicine, Syiah Kuala University
(3) Faculty of Veterinary Medicine, Syiah Kuala University
(4) Faculty of Veterinary Medicine, Syiah Kuala University
(5) Post Graduate Program of Veterinary Public Health, Syiah Kuala University
(6) Faculty of Veterinary Medicine, Syiah Kuala University
(7) Faculty of Veterinary Medicine, Syiah Kuala University
(8) Faculty of Veterinary Medicine, Syiah Kuala University
(9) Faculty of Veterinary Medicine, Syiah Kuala University
(10) Faculty of Veterinary Medicine, Syiah Kuala University
(11) Faculty of Veterinary Medicine, Syiah Kuala University
(12) Faculty of Veterinary Medicine, Syiah Kuala University
(*) Corresponding Author

Abstract


The objective of this research was to evaluate the anthelmintic activity of ethanolic extract of nuts Veitchia merrillii against intestinal nematode, Ascaridia galli. Phytochemical tests were performed for testing different chemicals group present in the extract. The effect of these extract were determined by in vitro based on inhibition of motility and mortality of worms. Amount of four worms were exposed in triplicate to each of phosphate buffered saline, 25 mg/mL, and 75 mg/mL crude ethanolic extract of V. merrillii, and 15 mg/mL albendazole. The motility of worm was observed on 9, 18, 27, and 36 hours interval. The mortality of worms were recognized by their straight flat appearance with no movements at the head and tail regions of the body. We found that the ethanolic extract of V. merrillii contains tannins, alkaloids, flavonoids, triterpenoids, and saponins neither steroid. Based on the in vitro trials conducted using above extract at 25 and 75 mg/mL concentration, the extract of V. merrillii showed anthelmintic effect. The extract of V. merrillii was effective at 75 mg/mL concentration only. The study indicated that it is potential to develop herbal-based anthelmintic to control A. galli in poutry.

Keywords


Veitchia merrillii, Ascaridia galli, motility, mortality, anthelmintics



References

Abdelqader, A., Qaralla, B., Al-Ramamneh, D. and Das, G. 2012. Anthelmintic effects of citrus peels ethanolic extracts against Ascaridia galli. Vet. Parasitol. 188(2): 78-84.

Ademola, I.O. and Eloff, J.N. 2011. Anthelminthic activity of acetone extract and fractions of Vernonia amygdalina against Haemonchus contortus eggs and larvae. Trop. Anim. Health Prod. 43: 521–527.

Ajaiyeoba, E.A., Onocha, P.A. and Olarenwaju, O.T. 2001. In vitro anthelmintic properties of Buchholzia coriaceae and Gynandropsis gynandra extracts. Pharmaceut. Biol. 39(3): 217-220.

Ali, N., Shah, S.W.A., Shah, I., Ahmed, G., Ghias, M. and Khan, I. 2011. Cytotoxic and anthelmintic potential of crude saponins isolated from Achillea Wilhelmsii C. Koch and Teucrium Stocksianum boiss. BMC Compl. Altern. Med. 11(106): 1-7.

Alrubaie, A.L. 2015. Effect of alchoholic extract of Curcuma longa on Ascaridia infestation affecting chickens. Indian J. Experiment. Biol. 53: 452-456.

Atawodi, S.E. and Atawodi J.C. 2009. Azadirachta indica (Neem): a plant of multiple biological and pharmacological activities. Phytochem. Rev. 8: 601–620.

Bachaya, H.A., Iqbal, Z., Khan, M.N., Shindu, Z.D. and Jabbar, A. 2009. Anthelmintic activity of Ziziphus nummularia (bark) and Acacia nilotica (fruit) against Trichostrongylid nematodes of sheep. J. Ethnopharmacol. 123: 325–329.

Balqis, U., Darmawi, Maryam, Muslina, Hamzah, A., Daud, R., Hambal, M., Rinidar, Harris, A., Muttaqien, Azhar, dan Eliawardani. 2016. Motilitas Ascaridia galli dewasa dalam larutan ekstrak etanol biji palem putri (Veitchia merrillii). Agripet, 16(1): 9-15.

Chafton, L.A. 2006. The effect of a condensed tannin containing forage, Sericea lespedeza, on existing and challenge infections of Haemonchus contortus in sheep. Thesis. The Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College.

El-Sherbini, G.T. and Osman, S.M. 2013. Anthelmintic activity of unripe Mangifera indica L. (mango) against Strongyloides stercoralis. Int. J. Curr. Microbiol. App. Sci., 2(5): 401-409.

Hoet, S., Pieters, L., Muccioli, G.G., Habib-Jiwan, J.L., Opperdoes, F.R. and Quetin-Leclercq, J. 2007. Antitrypanosomal activity of triterpenoids and sterols from the leaves of Strychnos spinosa and related compounds. J. Nat. Prod. 70: 1360–1363.

Iqbal, Z., Sarwar, M., Jabbar, A., Ahmed, S., Nisa, M., Sajid, M.S., Khan, M.N., Mufti, K.A. and Yaseen, M. 2007. Direct and indirect anthelmintic effects of condensed tannins in sheep. Vet. Parasitol. 144: 125–131.

Islam, K.R., Farjana, T., Begum, N. and Mondal, M.M.H. 2008. In vitro efficacy of some indigenous plants on the inhibition of development of eggs of Ascaridia galli (Digenia: Nematoda), Bangladesh J. Vet. Med. 6(2): 159–167.

Jeyathilakan, N., Murali, K., Anandaraj, A., Latha, B.R., and Basith, S.A. 2010. Anthelmintic activity of essential oils of Cymbopogan nardus and Azadirachta indica on Fasciola gigantica. Tamilnadu J. Vet. Anim. Sci. 6(5): 204-209.

Jeyathilakan, N., Murali, K., Anandaraj, A., and Basith, S.A. 2011. In vitro evaluation of anthelmintic property of ethno-veterinary plant extracts against the liver fluke Fasciola gigantica. J. Parasitic Dis. 36(1): 26 – 30.

Jiraungkoorskul, W., Sahaphong, S., Tansatit, T., Kanwanrangsan, N. and Pipatshukiat, S. 2005. Eurytrema pancreaticum: the in vitro effect of praziquantel and triclabendazole on the adult fluke. Exp. Parasitol. 111: 172-177.

Khokon, J.U., Sharifuzzaman, Sarker, E.H., Rahman, M.A., Kisku, J.J. and Mustofa, M. 2014. Efficacy of neem leaf extract against ascariasis in indigenous chicken. Int. J. Nat. Soc. Sci. 1: 25-30.

Kundu, S., Roy, S., Lyndem, L.M. 2012. Cassia alata L: potential role as anthelmintic agent against Hymenolepis diminuta. Parasitology Research, 111: 1187–1192.

Kundu, S., and Lyndem L.M. 2013. In vitro screening for cestocidal activity of three species of cassia plants against the tapeworm Raillietina tetragona. J. Helminth. 87: 154–159.

Lakshmi, V., Joseph, S.K., Srivastava, S., Verma, S.K., Sahoo, M.K., Dube, V., Mishra, S.K., and Murthy, P.K. 2010. Antifilarial activity in vitro and in vivo of some flavonoids tested against Brugia malayi, Acta Trop. 116: 127–133.

Lateef, M., Iqbal, Z., Khan, M.N., Akhtar, M.S., and Jabbar, A. 2003. Anthelmintic activity of Adhatoda vesica roots. Int. J. Agri. Biol. 5(1): 86-90.

Lorenzi, H., Souza, H.M., Medeiros-Costa J.T., Cerqueira, L.S.C., and Ferreira, E. 2004. Palmeiras brasileiras e exóticas cultivadas. São Paulo: Nova Odessa. Instituto Plantarum de Estudos da Flora, p. 432.

Mali, R.G., and Mehta, A.A. 2008. A review on anthelmintics plants. Nat. Prod. Radiance, 7(5): 466-475.

Melzig, M.F., Bader, G. and Loose, R. 2001. Investigations of the mechanism of membrane activity of selected triterpenoid saponins. - 67: 43-48.

Monteiro, M.V.B., Bevilaqua, C.M.L., Morais, S.M., Machado, L.K.A., Camurça-Vasconcelos, A.L.F., Campello, C.C., Ribeiro, W.L.C. and Mesquita M. de A. 2011. Anthelmintic activity of Jatropha curcas L. seeds on Haemonchus contortus. Vet. Parasitol. 182: 259–263.

Ndjonka, D., Agyare, C., Lüersen, K., Djafsia, B., Achukwi, D., Nukenine, E.N., Hensel, A. and Liebau, E. 2011. In vitro activity of cameroonian and Ghanaian medicinal plants on parasitic (Onchocerca ochengi) and free-living (Caenorhabditis elegans) nematodes. J. Helminth. 85: 304–312.

Pal, D. and Pandap, K. 2010. Evaluation of anthelmintics activity of aerial part of Cynodon dactylon Pers. Ancient Sci. Life, 30(1): 12-13.

Rodríguez-Leyes, E.A., Vicente-Murillo, R., González-Canavaciolo, V.L., Sierra-Pérez, R.C., Marrero-Delange, D. and Leiva-Sánchez, Á.T. 2013. Contenido de ácidos grasos en la fracción lipídica de frutos de tres Arecaceae cultivadas en Cuba. Revista CENIC Ciencias Quí micas. 44: 23-28.

Saha, B.K, Abdullah-Al-Hasan, Md., Rahman, M.A., Hassan, Md. M. and Begum, N. 2015. Comparative efficacy of neem leaves extract and levamisole against ascariasis in chicken. Int. J. Nat. Soc. Sci. 2: 43-48.

Sarojini, N., Manjari, S.A. and Kanti C.C. 2011. Phytochemical screening and anthelmintcs activity study of Saraca indica leaves extracts. Int. Res. J. Pharm. 2(5): 194-197.

Siamba, D.N., Okitoi, L.O., Watai, M.K., Wachira, A.M., Lukibisi, F.B. and Mukisira, E.A. 2007. Efficacy of Tephrosia vogelli and Vernonia amygdalina as anthelmintics against Ascaridia galli in indigenous chicken. Livestock Res. Rur. Develop. 19(12): 8 pages.

Siddiqui, B.S., Afshan, F., Ghiasuddin, Faizi, S., Naqvi, S.N.H. and Tariq, R.M. 2000. Two insecticidal tetranortriterpenoids from A. indica. Phytochem. 53 (3): 371–376.

Silva, R.B., Silva-Jủnior, E.V., Rodrigues, L.C., Andrade, L.H.C., Silva, S.I.D., Harand, W. and Oliveira, A.F.M. 2015. A comparative study of nutritional composition and potential use of some underutilized tropical fruits of Arecaceae. An. Acad. Bras. Cienc. 1-10.

Vafaei, A. 2013. Antioxidant and cytotoxicity activities of Veitchia merrillii fruits, The 4th World Congres on Biotechnology, 23-25 Raleigh, North Carolina, USA.

Vidyadhar, S., Saidulu, M., Gopal, T.K., Chamundeeswari, D., Umamaheswara rao. and Banji, D. 2010. In vitro anthelmintic activity of the whole plant of Enicostemma littorale by using various extracts. Int. J. Appl. Biol. and Pharmaceut. Tech. 1(3): 1119-1125.

Zahir, A.A., Rahuman, A.A, Kamaraj, C., Bagavan, A., Elango, G., Sangaran, A. and Kumar, B.S. 2009. Laboratory determination of efficacy of indigenous plant extracts for parasites control. Parasitol. Res. 105(2):453-461.



DOI: https://doi.org/10.22146/tradmedj.12818

Article Metrics

Abstract views : 4056 | views : 13987

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2016 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada