DPPH RADICAL SCAVENGING ACTIVITY OF AQUEOUS FRACTION FROM ETHANOLIC EXTRACT OF TALOK FRUIT (Muntingia calabura L.)

https://doi.org/10.22146/tradmedj.10724

Tatang Irianti(1*), Yosi Bayu Murti(2), Damiana Nitya Kanistri(3), Desi Riza Pratiwi(4), Kuswandi Kuswandi(5), Ratih Anggar Kusumaningtyas(6)

(1) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Sekip Utara 55281
(2) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Sekip Utara 55281
(3) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Sekip Utara 55281
(4) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Sekip Utara 55281
(5) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Sekip Utara 55281
(6) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Sekip Utara 55281
(*) Corresponding Author

Abstract


The investigation of talok fruits (Muntingia calabura L.) was shown the antioxidant activity of aqueous fraction of the ethanolic extract is relatively low. Hydrolysis treatment has increased the antioxidant activity by releasing the flavonoid aglycone from glycoside form. This study aims to determine the effect of acid and alkaline hydrolysis, and hydrolysis time on the antioxidant activity of aqueous fraction of calabura fruits ethanolic extract. The antioxidant activity of acid hydrolyzed aqueous fractions in 1 and 3h hydrolysis, respectively 9.5 and 1.5 times more potent than the aqueous fraction, while the alkaline in 1 and 3h hydro-lysis were 2.5 and 6.5 times. Flavonoid aglycone liberated on acid hydrolysis and alkaline had different anti-oxidant activity. The value of IC50 by acid hydrolyzed aqueous fraction in 1h and 3h hydrolysis of 20.55 and 97.88μg/mL, while the alkaline in 1h and 3h hydrolysis of 66.64 and 25.53μg/mL. One hour acid hydrolysis had antioxidant activity greater than 3h whereas in alkaline the greatest antioxidant activity is shown in 3h.

Keywords


talok fruits (Muntingia calabura L.), antioxidant, DPPH, acid hydrolysis, alkaline hydrolysis



References

Alaniya, M.D., 1977, Kinetics of The Alkaline Hydrolysis of Flavonoid Glycosides, Khim. Prorodn. Soedin., 5, 646-649.

Amarowicz, R., Naczk, M., Shahidi, F., 2000, Antioxidant Activity of Crude Tannins of Canola and Rapeseed Hulls, JAOCS, 77, 957-961.

Annegowda, H. V., Nee, C. W., Mordi, M. N., Ramanathan, S., Mansor, S. M., 2010, Evaluation of Phenolic Content and Antioxidant Property of Hydrolysed Extracts of Terminalia catappa L. Leaf, Asian J. Plant Sci., 9 (8), 479-485.

Balsano, C., Alisi, A., 2009, Antioxidant Effect of Natural Bioactive Compounds, Curr. Pharm. Design, 15, 3063-3073.

Bambang, R., Sutrisno, 1986, Pereaksi KLT, Edisi I, Jakarta, Fakultas Farmasi Universitas Pancasila.

Bors, W., Heller, W., Michel, C., Saran, M., 1990, Flanonoid as Antioxidants: Determination of Radical Scavenging Efficiencies, Methd. Enzym., 186, 343-355.

Cao, G., Sofic, E., Prior, R.L., 1997, Antioxidant and Prooxidant Behavior of Flavonoids: Structure-Activity Relationships, Free Rad. Biol. Med., 22, 749-760.

Dai J., Mumper, R.J., 2010, Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties., Mol., 15, 7313-7352.

Dugas Jr., A.J., Castaneda-Acosta, J., Bonin, G.C., Price, K.L., Fischer, N.H., Winston, G.W., 2000, Evaluation of The Total Peroxyl Radical-Scavenging Capacity of Flavonoids: Structure-Activity Relationship, J. Nat. Products., 63, 327-331.

Geckil, H., Ates, B., Durmaz, G., Erdogan, S., Yilmaz, I., 2005, Antioxidant, Free Radical Scavenging and Metal Chelathing Characteristics of Propolis, American J. Biochem. Biotech., 1(1), 27-31.

Ghiselli, A., Nardini, M., Baldi, A., Scaccini, C., 1998, Antioxidant Activity of Different Phenolics Fractions Separated from an Italian Red Wine, J. Agric. Food Chem., 46, 361-367.

Halliwel, B., 2001, Free Radicals and Other Reactive Species in Disease, In: Nature Encylopedia of Life Sciences, 1-7, Nature Publishing Group, London.

Halliwel, B., Gutteridge, J., 1999, Free Radical in Biology and Medicine, 23-38, Oxford University Press, New York.

Harborne, J.B., 1965, Plant Polyphenols: Characterisation of Flavonoid Glycosides by Acidic and Enzymic Hydrolysis, Phytochem., 4, 107-120.

Harborne, J.B., Grayer, R.J., 1994, Flavonoids and Insects, In Flavonoids Advances in Research Since 1986, 589-618, Chapman & Hall, London. Hopia, A.,

Heinonen, M., 1999, Antioxidant Activity of Flavonol Aglycones and Their Glycosides in Methyl Linoleate, JAOCS, 76, 139-144.

Irianti T., Puspasari A., dan Choironi NA., 2013, Aktivitas Penangkapan Radikal 2,2-Difenil-pikrilhidrazil (DPPH) Oleh Ekstrak etanolik daun Mengkudu (Morinda citrifolia L.), Fraksi air dan fraksi air terhidrolisis, Jurnal Bahan Alam Indonesia, Vol. 8 (5): 302-309

Jacob, R. A., Burri, B. J., 1996, Oxidative Damage and Defense, Am. J. Clin. Nutr., 63, 985S-990S.

Jork, H., Funk, W., Fischer, W., Wimmer, H.,1990, Thin-Layer Chromatography: Reagents and Detection Methods, Volume 1a, VCH Publishing, Germany.

Kim JK., and Jang JH., 2010, the first total synthesis of 2,3,6-tribromo-4 ,5-dihydroxybenzyl methyl ether (TDB) and its antioxidant activity, Bull. Korean Chem Soc., 23 (5), 661-662

Kolar, F. R., Kamble, V. S., Dixit, G. B., 2011, Phytochemical Constituents and Antioxidant Potential of Some Underused Fruits, African J. Pharm. Pharmacol., 5(18), 2067-2072. Litvinenko, V.L., Makarov, V.A., 1969, The Alkaline Hydrolysis of Flavonoid Glycosides, Khim. Prorodn. Soedin., 5(5), 366-369.

Markham, K.R., 1988, Cara Mengidentifikasi Flavonoid, diterjemahkan oleh Kosasih Padmawinata, Penerbit ITB, Bandung. Molyneux, P., 2004, The Use of A Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity, Songklanarin J. Sci. Technol., 26(2), 211-219.

Nollet, L.M.L., Toldra, F., 2012, Handbook of Analysis of Active Compounds in Functional Foods, CRC Press Taylor & Francis Group, FL.

Osawa, T., Namiki, M., 1981, A Novel Type Antioxidant Isolated from Leaf Wax of Eucalyptus Leaves, J. Agric. Biol. Chem., 45, 735-739.

Pervical, M., 1998. Antioxidants, Biochem. J., 252, 649-653. Pietta, P.G., 2000, Flavonoids as Antioxidants, J. Nat. Prod., 63, 1035-1042.

Plazonic, A., Bucar, F., Males, Z., Mornar, A., Nigovic, B., & Kujundzic, N. 2009, Identification and Quantification of Flavonoids and Phenolic Acids in Burr Parsley (Caucalis platycarpos L.), Using High-Performance Liquid Chromatography with Diode Array Detection and Electrospray Ionization Mass Spectrometry, Mol., 14(7), 2466–2490.

Preethi, K., Premasudha, P., Keerthana, K., 2012, Anti-Inflammatory Activity of Muntingia calabura Fruits, Phcog. J., 4(30), 51-56.

Preethi, K., Vijayalakshmi, N., Shamna, R., Sasikumar, J.M., 2010, In Vitro Antioxidant Activity of Extracts from Fruits of Muntingia calabura Linn. From India, Phcog. J., 2 (14), 11-18.

Rice Evans CA., Miller NJ., and Paganga G., 1996, Structure-antioxidant activity relationships of flavonoids and phenolic acids, Free Radic. Biol. Med., 21 (3): 417-421

Sani, I. M., Iqbal, S., Chan, K. W., Ismail, M., 2012, Effect of Acid and Base Catalyzed Hydrolysis on the Yield of Phenolics and Antioxidant Activity of Extracts from Germinated Brown Rice (GBR), Mol., 17, 7584-7594.

Sishelli, 1998, Antioxidants in Disease, Mechanisms, and Therapy, Academic Press, New York.

Sies, H., Stahl, W., 1995, Vitamins E and C, Beta Carotene and Caretenoids as Antioxidants, Amer. J. Clin., 62, 1315s-1321s.

Su, B.N., Park, E.J., Vigo, J.S., Graham, J.G., Cabieses, F., Fong, H.H.S., dkk., 2003, Activity-Guided Isolation of the Chemical Constituents of Muntingia calabura Using A Quinine Reductase Induction Assay, Phyto. Chem., 63, 335-341.

Tubesha, Z., Iqbal, S., Ismail, M., 2011, Effects of Hydrolysis Condition on Recovery of Antioxidants from Methanolic Extracts of Nigella sativa Seeds, J. Med. Plants Res., 5(22), 5292-5299.

Vinson, J. A., Proch, J., Zubik, L., 1999, Phenol Antioxidant Quantity and Quality in Foods: Cocoa Dark Chocolate and Milk Chocolate, J. Agric. Food Chem., 47(12), 4821-4824.

Wang, S.Y., Kuo, Y.H., Chang, H.N., Kang, P.L., Tsay, H.S., Lin, K.F., Yang, N.S., Shyur, N.F., 2002, Profiling and Characterization Antioxidant Activities in Anoectochilus formosanus Hayata, J. Agric. Food. Chem., 50, 1859-1865

Wagner, H., Bladt, S., Zgamski, E.M., 1984, Plant Drug Analysis A Thin Layer Chromatography Atlas, Springer Verlag, Berlin.

Zengin, G., Cakmak, Y.S., Guler, G.O., Aktumsek, A., 2010, In Vitro Antioxidant Capacities and Fatty Acid Compositions of Three Centaurea species Collected from Central Anatolia region of Turkey, Food Chem. Toxicol., 48, 2638-2641



DOI: https://doi.org/10.22146/tradmedj.10724

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