ACUTE TOXICITY TEST OF ROSELLA (Hibiscus sabdariffa L.) CALYX ETHANOLIC EXTRACT ON Sprague Dawley RATS

https://doi.org/10.22146/tradmedj.10673

Fita Sari(1*), Nurkhasanah Nurkhasanah(2), Moch. Saiful Bachri(3)

(1) Faculty of Pharmacy, Universitas Ahmad Dahlan, Jl. Prof. Dr. Soepomo, Janturan, Yogyakarta
(2) Faculty of Pharmacy, Universitas Ahmad Dahlan, Jl. Prof. Dr. Soepomo, Janturan, Yogyakarta
(3) Faculty of Pharmacy, Universitas Ahmad Dahlan, Jl. Prof. Dr. Soepomo, Janturan, Yogyakarta
(*) Corresponding Author

Abstract


Roselle (Hibiscus sabdariffa L.) was used as a traditional medicine because it has antioxidant activity. Traditional medicine used to treat a disease should consider the safety aspects through the toxicity tests. This study aims to determine the acute toxicity of the ethanolic extract from the calyx of Hibiscus sabdariffa L., activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and hepar histopathology in Sprague Dawley (SD) rats. Acute toxicity tests was done by orall administration of single dose ethanolic extract at dose 40, 200, 1000 and 5000 mg/kg BB. The test was observed during 14 days post treatment. The activity of AST, ALT and ALP were measured by specthrophotometer UV-Vis Diasys® method. The results showed that LD50 of ethanolic extract of calyx roselle was found at 850,90 mg/kg BW, the activity of AST, ALT, ALP did not different significantly in the treatment to be and control group (p <0.05) and histopathological data found structural change and tissue in hepar. Tthe delayed toxic effects until day 14 was also not found. These research found that ethanolic extract of calyx roselle was toxic SDrats.


Keywords


AST, ALT, ALP, calyx, obat herbal, rosella.



References

Adetutu, A., Owoade, A.O., 2013. Hepatoprotective And Antioxidant Effect Of Hibiscus Polyphenol Rich Extract (Hpe) Against Carbon Tetrachloride (Ccl4 ) –Induced Damage In Rats. Br. J. Med. Med. Res. 3(4), 1574–1586.

Adeyemi, D.O., Ukwenya, V.O., Obuotor, E.M., Adewole, S.O., 2014. Anti -Hepatotoxic Activities Of Hibiscus sabdariffa L. In Animal Model Of Streptozotocin Diabetes-Induced Liver Damage. Bmc Complement. Altern. Med. 14, 277. Doi:10.1186/1472-6882-14- 277.

Akuba, J., 2013. Efek Ekstrak Etanol Kelopak Bunga Rosela (Hibiscus sabdariffa L.) Terhadap Aktivitas Fagositosis , Sekresi Nitrit Oksida, Sekresi Reactive Oxigen Intermediate Dan Ekspresi Il-2 Makrofag Tikus Sprague Dawley Yang Diinduksi 7,12- Dimetilbenz Antrasen. Tesis.

Anonim, 2014. Peraturan Kepala Badan Pengawas Obat Dan Makanan Republik Indonesia Nomor 7 Tahun 2014 Tentang Pedoman Uji Toksisitas Nonklinik Secara In Vivo, 07 Ed.

Apsari Dwi, P., Susanti, H., 2011. Perbandingan Kadar Fenolik Total Ekstrak Metanol Kelopak Merah Dan Ungu Bunga Ros ella (Hibiscus sabdariffa, Linn) Secara Spektrofotometri. Pros. Seminar. Nasional. Home Care 4-7, 978–979.

Da-Costa-Rocha, I., Bonnlaender, B., Sievers, H., Pischel, I., Heinrich, M., 2014. Hibiscus sabdariffa L. - A Phytochemical And Pharmacological Review. Food Chem. 165, 424–443. Doi:10.1016/J.Foodchem.2014.05.002.

Hopkins, A.L., Lamm, M.G., Funk, J., Ritenbaugh, C., 2013. Hibiscus sabdariffa L. In The Treatment Of Hypertension And Hyperlipidemia: A Comprehensive Review Of Animal And Human Studies. Fitoterapia 85, 84–94. Doi:10.1016/J.Fitote.2013.01.003.

Isroli, S.S., 2014. Pengaruh Supl ementasi Jintan Hitam (Nigella sativa) Giling Terhadap Aspartate Aminotransferase (Ast), Alanine Aminotransferase (Alt) Dan Berat Organ Hati Broiler. J. Peternakan. Indonesia. 16 (1).

Liu, J.-Y., Chen, C.-C., Wang, W.-H., Hsu, J.-D., Yang, M.-Y., Wang, C.-J., 2006. The Protective Effects Of Hibiscus Sabdariffa Extract On Ccl4-Induced Liver Fibrosis In Rats. Food Chem. Toxicol. 44, 336–343. Doi:10.1016/J.Fct.2005.08.003.

Morales, G., Paredes, A., Olivares, A., Bravo, J., 2014. Acute Oral Toxicity And Anti- Inflamm ato ry Activity Of Hydroalcoholic

Extract From Lampaya medicinalis Phil In Rats. Biol. Res. 47, 6. Doi:10.1186/0717- 628 7-4 7- 6.

Nurkhasanah, Muhammad Ryan Radix Rahardhian, 2015. Hepatoprotective Effect Of Hibiscus sabdariffa L Extract On 7,12- Dimethylbenz(Α)Antracene (Dmba) Induced Rat. International Journal of Biological and Medical Research.

Ojeda, D., Enrique Jiménez-Ferrer, E., Enrique, A., Herrera-Arellano, A., Tortori ello, J., Alvarez, L., 2010. Inhibition Of Angiotensin Convertin Enzyme (Ace) Activity By The Anthocyanins Delphinidin- And Cyanidin-3- O-Sambubiosides From Hibiscus sabdariffa L. Journal of Ethnopharmacology.

Sireeratawong, S., 2013. Toxicity Studies Of The Water Extract From The Calyces Of Hibiscus sabdariffa L. In Rats. Afr J Tradit Complement Altern Med 2013 104122-127 10 (4), 122–127.

Siregar, R.N.I., 2015. The Effect O F Eugenia P Olyantha Extract On Ldl Cholesterol. J Major. 4 Nomor 5, 85–90.



DOI: https://doi.org/10.22146/tradmedj.10673

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